Construct: ORF TRCN0000491630
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012233.1_s317c1
- Derived from:
- ccsbBroadEn_02621
- DNA Barcode:
- GCTATCAAATGTTTGTGAAGCGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRDM7 (11105)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491630
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11105 | PRDM7 | PR/SET domain 7 | XM_017022884.1 | 54.6% | 38.8% | 1_81del;411_693del;878_939del |
| 2 | human | 11105 | PRDM7 | PR/SET domain 7 | XM_011522829.1 | 45.3% | 45.3% | 1_618del |
| 3 | human | 11105 | PRDM7 | PR/SET domain 7 | XM_017022882.1 | 40.8% | 29.2% | 1_399del;729_1011del;1196_1257del |
| 4 | human | 11105 | PRDM7 | PR/SET domain 7 | XM_017022883.1 | 40.8% | 29.2% | 1_399del;729_1011del;1196_1257del |
| 5 | human | 11105 | PRDM7 | PR/SET domain 7 | NM_001098173.1 | 34.7% | 24.9% | 1_618del;948_1230del;1415_1476del |
| 6 | human | 11105 | PRDM7 | PR/SET domain 7 | XM_005256274.3 | 34.7% | 24.9% | 1_618del;948_1230del;1415_1476del |
| 7 | human | 56979 | PRDM9 | PR/SET domain 9 | NM_001310214.1 | 18.5% | 13.8% | (many diffs) |
| 8 | human | 56979 | PRDM9 | PR/SET domain 9 | NM_020227.3 | 18.5% | 13.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 582
- ORF length:
- 513
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgtcagaac ttcttcattg acagctgtgc tgctcatggg ccccctacat 121 ttgtaaagga cagtgcagtg gacaaggggc atcccaaccg ttcagccctc agtctgcccc 181 cggggctgag aattgggcca tcaggcatcc ctcaggctgg gcttggagta tggaacgagg 241 catcTGATCT GCCACTGGGT CTGCACTTTG GCCCCTATGA GGGCCGAATT ACAGAAGACG 301 AAGAGGCAGC CAACAGTGGA TATTCCTGGC TAATCACCAA GGGGAGAAAC TGCTATGAGT 361 ATGTGGATGG AAAAGATAAA TCCTCGGCCA ACTGGATGAG AACCAAAGCC AGAGATCCAT 421 CCATGTCCCT CATGCTGTCT GGCCTTTTCA AGTCAAAAAT TTCTCAGTCA ACATGTGGAA 481 CGCAATCACT CCTCTCAGAA CTTCCCAGGA CCATCTGCAA GAAAACTTCT CCAACCAGAG 541 AATCCCTGCC CAGGGGATCA GAATCAGGAG CGGCAATATT CTTGCCAACT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGAGCT ATCAAATGTT TGTGAAGCGC CACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t