Construct: ORF TRCN0000491633
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020262.2_s317c1
- DNA Barcode:
- CGTCGTACCCTGACTGTAAATATA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STK24 (8428)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491633
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8428 | STK24 | serine/threonine kinase 24 | NM_001032296.3 | 99.9% | 99.7% | 766A>G |
| 2 | human | 8428 | STK24 | serine/threonine kinase 24 | NM_003576.4 | 95.9% | 94.8% | (many diffs) |
| 3 | human | 8428 | STK24 | serine/threonine kinase 24 | NM_001286649.1 | 95.5% | 95.3% | 273_274ins57;709A>G |
| 4 | human | 8428 | STK24 | serine/threonine kinase 24 | XM_017020794.2 | 82.6% | 81.4% | (many diffs) |
| 5 | human | 8428 | STK24 | serine/threonine kinase 24 | XM_005254079.5 | 77.1% | 77% | 0_1ins294;472A>G;774G>A |
| 6 | human | 8428 | STK24 | serine/threonine kinase 24 | XM_024449426.1 | 77.1% | 77% | 0_1ins294;472A>G;774G>A |
| 7 | mouse | 223255 | Stk24 | serine/threonine kinase 24 | NM_145465.2 | 88% | 97.4% | (many diffs) |
| 8 | mouse | 223255 | Stk24 | serine/threonine kinase 24 | XM_011245043.2 | 86.1% | 94.6% | (many diffs) |
| 9 | mouse | 223255 | Stk24 | serine/threonine kinase 24 | XM_017315988.1 | 84.9% | 91.9% | (many diffs) |
| 10 | mouse | 223255 | Stk24 | serine/threonine kinase 24 | XM_006518918.1 | 67.5% | 74.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1365
- ORF length:
- 1293
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctcac tccccggtgc agtcgggcct gcccggcatg cagaacctaa 121 aggcagaccc agaagagctt tttacaaaac tagagaaaat tgggaagggc tcctttggag 181 aggtgttcaa aggcattgac aatcggactc agaaagtggt tgccataaag atcattgatc 241 tggaagaagc tgaagatgag atagaggaca ttcaacaaga aatcacagtg ctgagtcagt 301 gtgacagtcc atatgtaacc aaatattatg gatcctatct gaaggataca aaattatgga 361 taataatgga atatcttggt ggaggctccg cactagatct attagaacct ggcccattag 421 atgaaaccca gatcgctact atattaagag aaatactgaa aggactcgat tatctccatt 481 cggagaagaa aatccacaga gacattaaag cggccaacgt cctgctgtct gagcatggcg 541 aggtgaagct ggcggacttt ggcgtggctg gccagctgac agacacccag atcaaaagga 601 acaccttcgt gggcacccca ttctggatgg cacccgaggt catcaaacag tcggcctatg 661 actcgaaggc agacatctgg tccctgggca taacagctat tgaacttgca agaggggaac 721 cacctcattc cgagctgcac cccatgaaag ttttattcct cattccaaag aacaacccac 781 cgacgttgga aggaaactac agtaaacccc tcaaggagtt tgtggaggcc tgtttggata 841 aggagccgag ctttagaccc actgctaagg agttattgaa gcacaagttt atactacgca 901 atgcaaagaa aacttcctac ttgaccgagc tcatcgacag gtacaagaga tggaaggccg 961 agcagagcca tgacgactcg agctccgagg attccgacgc ggaaacagat ggccaagcct 1021 cggggggcag tgattcTGGG GACTGGATCT TCACAATCCG AGAAAAAGAT CCCAAGAATC 1081 TCGAGAATGG AGCTCTTCAG CCATCGGACT TGGACAGAAA TAAGATGAAA GACATCCCAA 1141 AGAGGCCTTT CTCTCAGTGT TTATCTACAA TTATTTCTCC TCTGTTTGCA GAGTTGAAGG 1201 AGAAGAGCCA GGCGTGCGGA GGGAACTTGG GGTCCATTGA AGAGCTGCGA GGGGCCATCT 1261 ACCTAGCGGA GGAGGCGTGC CCTGGCATCT CCGACACCAT GGTGGCCCAG CTCGTGCAGC 1321 GGCTCCAGAG ATACTCTCTA AGTGGTGGAG GAACTTCATC CCACTGAGAC CCAGCTTTCT 1381 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1441 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1501 GTGGAAAGGA CGACGTCGTA CCCTGACTGT AAATATAACG CGTTAAGTCg acaatcaacc 1561 tctggattac aaaatttgtg aaagatt