Transcript: Human NM_003576.4

Homo sapiens serine/threonine kinase 24 (STK24), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-05
Taxon:
Homo sapiens (human)
Gene:
STK24 (8428)
Length:
4579
CDS:
274..1605

Additional Resources:

NCBI RefSeq record:
NM_003576.4
NBCI Gene record:
STK24 (8428)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001144978 TGACAATCGGACTCAGAAAG pXPR_003 TGG 178 13% 2 0.4142 STK24 STK24 77056
2 BRDN0001146213 AATACTTACTAGATCTAGTG pXPR_003 CGG 358 27% 3 0.4078 STK24 STK24 77057
3 BRDN0001147108 CTGTAGTTTCCTTCCAACGT pXPR_003 CGG 750 56% 6 -0.0404 STK24 STK24 77055
4 BRDN0001144931 ACAGCTATTGAACTTGCAAG pXPR_003 AGG 674 51% 6 -0.3024 STK24 STK24 77058
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_003576.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195637 CCCACTGCTAAGGAGTTATTG pLKO.1 1096 CDS 100% 13.200 18.480 N STK24 n/a
2 TRCN0000342500 CCCACTGCTAAGGAGTTATTG pLKO_005 1096 CDS 100% 13.200 18.480 N STK24 n/a
3 TRCN0000000641 GCAGGGTTTGTCATTAATAAT pLKO.1 2234 3UTR 100% 15.000 10.500 N STK24 n/a
4 TRCN0000194713 CAATTATTTCTCCTCTGTTTG pLKO.1 1406 CDS 100% 10.800 7.560 N STK24 n/a
5 TRCN0000199367 CCCTGGGCATAACAGCTATTG pLKO.1 920 CDS 100% 10.800 7.560 N STK24 n/a
6 TRCN0000342448 CCCTGGGCATAACAGCTATTG pLKO_005 920 CDS 100% 10.800 7.560 N STK24 n/a
7 TRCN0000194746 CCAAATATTATGGATCCTATC pLKO.1 557 CDS 100% 6.000 4.200 N STK24 n/a
8 TRCN0000000643 GACAGGTACAAGAGATGGAAG pLKO.1 1174 CDS 100% 4.050 2.835 N STK24 n/a
9 TRCN0000342450 GACAGGTACAAGAGATGGAAG pLKO_005 1174 CDS 100% 4.050 2.835 N STK24 n/a
10 TRCN0000000642 ACAGAGACATTAAAGCGGCCA pLKO.1 734 CDS 100% 0.540 0.378 N STK24 n/a
11 TRCN0000000644 TGGACAGAAATAAGATGAAAG pLKO.1 1349 CDS 100% 10.800 6.480 N STK24 n/a
12 TRCN0000000645 TGCAGAGTTGAAGGAGAAGAG pLKO.1 1425 CDS 100% 4.050 2.430 N STK24 n/a
13 TRCN0000342451 TGCAGAGTTGAAGGAGAAGAG pLKO_005 1425 CDS 100% 4.050 2.430 N STK24 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_003576.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14893 pDONR223 0% 96% 95% None (many diffs) n/a
2 ccsbBroad304_14893 pLX_304 0% 96% 95% V5 (many diffs) n/a
3 TRCN0000469460 CTTGGGCACCAGGCGCTTACTTTC pLX_317 25.5% 96% 95% V5 (many diffs) n/a
4 ccsbBroadEn_07238 pDONR223 100% 95.9% 94.8% None (many diffs) n/a
5 ccsbBroad304_07238 pLX_304 0% 95.9% 94.8% V5 (many diffs) n/a
6 TRCN0000480096 CACCCCGGTGCCGAGCGGTCCGCT pLX_317 29.1% 95.9% 94.8% V5 (many diffs) n/a
7 TRCN0000491633 CGTCGTACCCTGACTGTAAATATA pLX_317 25.8% 95.9% 94.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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