Transcript: Mouse NM_145465.2

Mus musculus serine/threonine kinase 24 (Stk24), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Stk24 (223255)
Length:
2575
CDS:
292..1587

Additional Resources:

NCBI RefSeq record:
NM_145465.2
NBCI Gene record:
Stk24 (223255)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001144978 TGACAATCGGACTCAGAAAG pXPR_003 TGG 142 11% 2 0.2882 Stk24 STK24 77056
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_145465.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000276662 TCCCTTGGCATCACCGCAATA pLKO_005 901 CDS 100% 10.800 15.120 N Stk24 n/a
2 TRCN0000025240 CGAGCTTATCGACAGGTACAA pLKO.1 1146 CDS 100% 4.950 6.930 N Stk24 n/a
3 TRCN0000025243 CCAAGCTAGAGAAGATTGGAA pLKO.1 365 CDS 100% 3.000 4.200 N Stk24 n/a
4 TRCN0000025239 GCAACCATATTACGAGAAATT pLKO.1 655 CDS 100% 13.200 10.560 N Stk24 n/a
5 TRCN0000025241 GCCTGGCCCTTTAGATGAAAT pLKO.1 627 CDS 100% 13.200 10.560 N Stk24 n/a
6 TRCN0000276725 GCCTGGCCCTTTAGATGAAAT pLKO_005 627 CDS 100% 13.200 10.560 N Stk24 n/a
7 TRCN0000025242 CTCTCAGTGTTTATCCACAAT pLKO.1 1371 CDS 100% 4.950 3.960 N Stk24 n/a
8 TRCN0000276721 CTCTCAGTGTTTATCCACAAT pLKO_005 1371 CDS 100% 4.950 3.960 N Stk24 n/a
9 TRCN0000276660 CCTACGTCACCAAGTACTATG pLKO_005 530 CDS 100% 10.800 7.560 N Stk24 n/a
10 TRCN0000276723 TCCTATCTCAAGGATACTAAG pLKO_005 553 CDS 100% 10.800 7.560 N Stk24 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_145465.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14893 pDONR223 0% 88.1% 97.6% None (many diffs) n/a
2 ccsbBroad304_14893 pLX_304 0% 88.1% 97.6% V5 (many diffs) n/a
3 TRCN0000469460 CTTGGGCACCAGGCGCTTACTTTC pLX_317 25.5% 88.1% 97.6% V5 (many diffs) n/a
4 ccsbBroadEn_07238 pDONR223 100% 88% 97.4% None (many diffs) n/a
5 ccsbBroad304_07238 pLX_304 0% 88% 97.4% V5 (many diffs) n/a
6 TRCN0000480096 CACCCCGGTGCCGAGCGGTCCGCT pLX_317 29.1% 88% 97.4% V5 (many diffs) n/a
7 TRCN0000491633 CGTCGTACCCTGACTGTAAATATA pLX_317 25.8% 88% 97.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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