Construct: ORF TRCN0000491637

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021819.2_s317c1
DNA Barcode:
CTCGAAGCCATCATTCTCCAGACG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PPIA (5478)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491637
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5478 PPIA peptidylprolyl isomerase A NM_021130.5 100% 100%
2 human 729739 PPIAP46 peptidylprolyl isomerase A ... NR_045207.1 93.5% (many diffs)
3 human 653598 PPIAL4C peptidylprolyl isomerase A ... NM_001135789.3 91.9% 86% (many diffs)
4 human 653505 PPIAL4A peptidylprolyl isomerase A ... NM_001143883.3 91.5% 85.4% (many diffs)
5 human 644591 PPIAL4G peptidylprolyl isomerase A ... NM_001123068.1 91.3% 84.2% (many diffs)
6 human 105371242 PPIAL4H peptidylprolyl isomerase A ... NM_001368128.1 91.1% 84.2% (many diffs)
7 human 730262 PPIAL4E peptidylprolyl isomerase A ... NM_001144032.2 90.9% 84.2% (many diffs)
8 human 728945 PPIAL4F peptidylprolyl isomerase A ... NM_001164262.2 90.9% 84.2% (many diffs)
9 human 645142 PPIAL4D peptidylprolyl isomerase A ... NM_001164261.1 90.7% 83.6% (many diffs)
10 human 5478 PPIA peptidylprolyl isomerase A XM_024446808.1 89% 81.7% (many diffs)
11 human 100192204 PPIAP30 peptidylprolyl isomerase A ... NR_036506.1 71.2% (many diffs)
12 human 5478 PPIA peptidylprolyl isomerase A NM_001300981.2 63.6% 63.6% 0_1ins180
13 human 5478 PPIA peptidylprolyl isomerase A XM_024446809.1 63.6% 63.6% 0_1ins180
14 human 5478 PPIA peptidylprolyl isomerase A XR_002956460.1 29% 1_765del;952_1172del;1482_1704del
15 human 284996 RNF149 ring finger protein 149 XR_001738713.2 16.5% (many diffs)
16 mouse 268373 Ppia peptidylprolyl isomerase A NM_008907.1 91.9% 95.7% (many diffs)
17 mouse 668548 Gm9234 predicted pseudogene 9234 XR_869917.3 60.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
567
ORF length:
495
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggtcaac cccaccgtgt tcttcgacat tgccgtcgac ggcgagccct
121tgggccgcgt ctcctttgag ctgtttgcag acaaggtccc aaagacagca gaaaattttc
181gtgctctgag cactggagag aaaggatttg gttataaggg ttcctgcttt cacagaatta
241ttccagggtt tatgtgtcag ggtggtgact tcacacgcca taatggcact ggtggcaagt
301ccatctatgg ggagaaattt gaagatgaga acttcatcct aaagcatacg ggtcctggca
361tcttgtccat ggcaaatgct ggacccaaca caaatggttc ccagtttttc atcTGCACTG
421CCAAGACTGA GTGGTTGGAT GGCAAGCATG TGGTGTTTGG CAAAGTGAAA GAAGGCATGA
481ATATTGTGGA GGCCATGGAG CGCTTTGGGT CCAGGAATGG CAAGACCAGC AAGAAGATCA
541CCATTGCTGA CTGTGGACAA CTCGAATAGA ACCCAGCTTT CTTGTACAAA GTGGTTGATA
601TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
661CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTCGA
721AGCCATCATT CTCCAGACGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
781tgaaagatt

Download FASTA (ORF) (Full)