Construct: ORF TRCN0000491649
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019109.2_s317c1
- DNA Barcode:
- GGCGACGAAAAACCGGATATGAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CDK18 (5129)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491649
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5129 | CDK18 | cyclin dependent kinase 18 | NM_212503.3 | 68.4% | 83.8% | 276A>G;1310_1311ins695;1512_1513insG |
| 2 | human | 5129 | CDK18 | cyclin dependent kinase 18 | NM_002596.4 | 64.4% | 78.1% | 273_274ins90;1220_1221ins695;1422_1423insG |
| 3 | human | 5129 | CDK18 | cyclin dependent kinase 18 | NM_212502.3 | 64.4% | 78.1% | 273_274ins90;1220_1221ins695;1422_1423insG |
| 4 | human | 5129 | CDK18 | cyclin dependent kinase 18 | XM_017001423.2 | 62.6% | 74.2% | (many diffs) |
| 5 | human | 5129 | CDK18 | cyclin dependent kinase 18 | XR_002956776.1 | 60.1% | 1_40del;316A>G;2248_3665delinsG | |
| 6 | human | 5129 | CDK18 | cyclin dependent kinase 18 | XR_426781.4 | 59.5% | 1_81del;357A>G;2289_3706delinsG | |
| 7 | human | 5129 | CDK18 | cyclin dependent kinase 18 | XM_011509602.3 | 58.9% | 69.2% | (many diffs) |
| 8 | human | 5129 | CDK18 | cyclin dependent kinase 18 | XR_426780.4 | 57.3% | 1_223del;499A>G;2431_3848delinsG | |
| 9 | human | 5129 | CDK18 | cyclin dependent kinase 18 | XR_426779.3 | 56.1% | 1_307del;583A>G;2515_3932delinsG | |
| 10 | human | 5129 | CDK18 | cyclin dependent kinase 18 | XR_426784.3 | 55.4% | 1_195del;468_469ins90;2313_3730delinsG | |
| 11 | human | 5129 | CDK18 | cyclin dependent kinase 18 | XR_426782.4 | 55% | 1_223del;496_497ins90;2341_3758delinsG | |
| 12 | human | 5129 | CDK18 | cyclin dependent kinase 18 | XR_001737212.2 | 54.6% | 1_415del;691A>G;2623_4040delinsG | |
| 13 | human | 5129 | CDK18 | cyclin dependent kinase 18 | XR_001737213.1 | 53.8% | 1_307del;580_581ins90;2425_3842delinsG | |
| 14 | human | 5129 | CDK18 | cyclin dependent kinase 18 | XR_921822.3 | 52.4% | 1_411del;684_685ins90;2529_3946delinsG | |
| 15 | mouse | 18557 | Cdk18 | cyclin-dependent kinase 18 | NM_008795.2 | 54.7% | 70.2% | (many diffs) |
| 16 | mouse | 18557 | Cdk18 | cyclin-dependent kinase 18 | XM_011247940.2 | 45.9% | 58.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1590
- ORF length:
- 1518
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgatcatg aacaagatga agaactttaa gcgccgtttc tccctgtcag 121 tgccccgcac tgagaccatt gaagaatcct tggctgaatt cacggagcaa ttcaaccagc 181 tccacaaccg gcggaatgag aacttgcagc tcggtcctct tggcagagac cccccgcagg 241 agtgcagcac cttctcccca acagacagcg gggaggagcc ggggcagctc tcccctggcg 301 tgcagttcca gcggcggcag aaccagcgcc gcttctccat ggaggtgagg gcctctggag 361 ctctgccccg gcaggtggca ggatgcacgc acaagggtgt gcacaggagg gcagctgcct 421 tacagccaga ctttgacgtc agcaagaggc tctctctgcc catggatatc cgcctgcccc 481 aggaattcct acagaagcta cagatggaga gcccagatct gcccaagccg ctcagccgca 541 tgtcccgccg ggcctccctg tcagacattg gctttgggaa actggaaaca tacgtgaaac 601 tggacaaact gggagagggc acctatgcca cagtcttcaa agggcgcagc aaactgacgg 661 agaaccttgt ggccctgaaa gagatccggc tggagcacga ggagggagcg ccctgcactg 721 ccatccgaga ggtgtctctg ctgaagaacc tgaagcacgc caatattgtg accctgcatg 781 acctcatcca cacagatcgg tccctcaccc tggtgtttga gtacctggac agtgacctga 841 agcagtatct ggaccactgt gggaacctca tgagcatgca caacgtcaag attttcatgt 901 tccagctgct ccggggcctc gcctactgtc accaccgcaa gatcctgcac cgggacctga 961 agccccagaa cctgctcatc aacgagaggg gggagctgaa gctggccgac tttggactgg 1021 ccagggccaa gtcagtgccc acaaagactt actccaatga ggtggtgacc ctgtggtaca 1081 ggccccccga tgtgctgctg ggatccacag agtactccac ccccattgat atgtggggcg 1141 tgggctgcat ccactacgag atggccacag ggaggcccct cttcccgggc tccacagtca 1201 aggaggagct gcacctcatc tttcgcctcc tcgggacccc cacagaagag acgtggcccg 1261 gcgtgaccgc cttctctgag ttccgcacct acagcttccc ctgctacctc ccgcagccgc 1321 tcatcaacca cgcgcccagg ttggatacgg atggcatcca cctcctgagc agcctgctcc 1381 tggtgagtgt cctcccggcg gggcccaggg agaggcagcc aaagagccag gtccctgtgc 1441 cccatgccgg gcctacgccc caacttctgt ggctcgtgtg cttacacgtc tttgaaacat 1501 ctcccctgta ctctccaccc ccaaggccaa ggggtggcct ccatacacat cctctggagt 1561 ctgtgcactg cgagcccaaa cccgttactt agctgtgtag ctctgggcaa gttacagagc 1621 tcctccatgc ctcagtttcc tcagctgcaa agtgggatta atacctattt tataagatgg 1681 ccgtgaggat acattgagat gacgggacac acttagtgtg gtgcctgtct tgtaaggcac 1741 acgatgaagg gttgttatcg gcactacgct tcctgttacg gatgttctcc atctgatcat 1801 ggttggtttg catctgtatt tctatcccaa gagaaggtca ctctcctgaa gtttcatttc 1861 tggtttttcc tggtggctgg agaccgagga gccttcccct gagtcttgag atggcctgag 1921 gttgggtttg tggtaggggc tggaggctga cctggggctt cTCCTGTTAG CCTCAAGCCT 1981 CACCCACCTT GGTGCTCTAA CCAGCACTGG AGTCCTGCTG CCCAGAGGGT TTGCAGCCCC 2041 CCAGCCGGGC CCAATAGCCC CACCCTGTGC CTTTCAGTAT GAATCCAAGA GTCGCATGTC 2101 AGCAGAGGCT GCCCTGAGTC ACTCCTACTT CCGGTCTCTG GGAGAGCGTG TGCACCAGCT 2161 TGAAGACACT GCCTCCATCT TCTCCCTGAA GGAGATCCAG CTCCAGAAGG ACCCAGGCTA 2221 CCGAGGCTTG GCCTTCCAGC AGCCAGGACG AGGGAAGAAC AGGCGGCAGA GCATCTTCGA 2281 CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG 2341 TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT 2401 TTATATATCT TGTGGAAAGG ACGAGGCGAC GAAAAACCGG ATATGAGTAC GCGTTAAGTC 2461 gacaatcaac ctctggatta caaaatttgt gaaagatt