Transcript: Human XM_017001423.2

PREDICTED: Homo sapiens cyclin dependent kinase 18 (CDK18), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CDK18 (5129)
Length:
3331
CDS:
205..1923

Additional Resources:

NCBI RefSeq record:
XM_017001423.2
NBCI Gene record:
CDK18 (5129)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148613 AAAGACTTACTCCAATGAGG pXPR_003 TGG 988 65% 10 0.8659 CDK18 CDK18 77881
2 BRDN0001144740 CATGAGCATGCACAACGTCA pXPR_003 AGG 814 54% 8 0.4521 CDK18 CDK18 77880
3 BRDN0001147494 AGCCTCTTGCTGACGTCCTG pXPR_003 TGG 363 24% 4 -0.4034 CDK18 CDK18 77879
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017001423.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000006334 GCCCACAAAGACTTACTCCAA pLKO.1 1374 CDS 100% 2.640 3.696 N CDK18 n/a
2 TRCN0000006333 GCCTTAAATTGGCAGGTGGTA pLKO.1 2704 3UTR 100% 2.640 3.696 N CDK18 n/a
3 TRCN0000315410 AGATCACATGGAGCACAAATT pLKO_005 1957 3UTR 100% 13.200 9.240 N CDK18 n/a
4 TRCN0000350588 GAACCTGAAGCACGCCAATAT pLKO_005 1083 CDS 100% 13.200 9.240 N CDK18 n/a
5 TRCN0000315411 GCATGCACAACGTCAAGATTT pLKO_005 1211 CDS 100% 13.200 9.240 N CDK18 n/a
6 TRCN0000381363 ACAGTGACCTGAAGCAGTATC pLKO_005 1166 CDS 100% 10.800 7.560 N CDK18 n/a
7 TRCN0000379497 ATGCACACGGATGACAGAATC pLKO_005 2239 3UTR 100% 10.800 7.560 N CDK18 n/a
8 TRCN0000350531 CAGCCTGCTCCTGTATGAATC pLKO_005 1707 CDS 100% 10.800 7.560 N CDK18 n/a
9 TRCN0000195355 CATACGTGAAACTGGACAAAC pLKO.1 926 CDS 100% 10.800 7.560 N CDK18 n/a
10 TRCN0000381142 GCTCTCTCTGCCCATGGATAT pLKO_005 786 CDS 100% 10.800 7.560 N CDK18 n/a
11 TRCN0000380996 TGGAGCCCACACACGTTTCAT pLKO_005 2358 3UTR 100% 5.625 3.938 N CDK18 n/a
12 TRCN0000199410 CCACGGCTGTTTCTTCTTTGT pLKO.1 2066 3UTR 100% 4.950 3.465 N CDK18 n/a
13 TRCN0000006336 CGCACTGAGACCATTGAAGAA pLKO.1 463 CDS 100% 4.950 3.465 N CDK18 n/a
14 TRCN0000006335 GAAACTGGAAACATACGTGAA pLKO.1 915 CDS 100% 4.050 2.835 N CDK18 n/a
15 TRCN0000315409 GAAACTGGAAACATACGTGAA pLKO_005 915 CDS 100% 4.050 2.835 N CDK18 n/a
16 TRCN0000199950 GAACCTCATGAGCATGCACAA pLKO.1 1200 CDS 100% 4.050 2.835 N CDK18 n/a
17 TRCN0000006337 GCGCAGCAAACTGACGGAGAA pLKO.1 981 CDS 100% 1.350 0.945 N CDK18 n/a
18 TRCN0000199711 GCACCTCATCTTTCGCCTCCT pLKO.1 1548 CDS 100% 0.720 0.504 N CDK18 n/a
19 TRCN0000199592 GCGCCGTTTCTCCCTGTCAGT pLKO.1 438 CDS 100% 0.000 0.000 N CDK18 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017001423.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14732 pDONR223 0% 82.4% 82.3% None 1_210del;478_567del;791C>T n/a
2 ccsbBroad304_14732 pLX_304 0% 82.4% 82.3% V5 1_210del;478_567del;791C>T n/a
3 TRCN0000466439 TTTGATTTCATTTCTTTCGAACAA pLX_317 22.4% 82.4% 82.3% V5 1_210del;478_567del;791C>T n/a
4 TRCN0000488438 TGGGCAGAAAGGATGCTTCTAAAC pLX_317 22.5% 82.4% 82.3% V5 (not translated due to prior stop codon) 1_210del;478_567del;791C>T n/a
5 ccsbBroadEn_10440 pDONR223 100% 82.3% 81.9% None (many diffs) n/a
6 ccsbBroad304_10440 pLX_304 0% 82.3% 81.9% V5 (many diffs) n/a
7 TRCN0000467401 ATTCAGTCAAGCCTAAACTATCTA pLX_317 26.3% 82.3% 81.9% V5 (many diffs) n/a
8 TRCN0000491649 GGCGACGAAAAACCGGATATGAGT pLX_317 14.6% 62.6% 74.2% V5 (not translated due to prior stop codon) (many diffs) n/a
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