Transcript: Human XR_001737213.1

PREDICTED: Homo sapiens cyclin dependent kinase 18 (CDK18), transcript variant X10, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CDK18 (5129)
Length:
3842
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001737213.1
NBCI Gene record:
CDK18 (5129)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001737213.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000006334 GCCCACAAAGACTTACTCCAA pLKO.1 1183 3UTR 100% 2.640 3.696 N CDK18 n/a
2 TRCN0000006333 GCCTTAAATTGGCAGGTGGTA pLKO.1 3208 3UTR 100% 2.640 3.696 N CDK18 n/a
3 TRCN0000315410 AGATCACATGGAGCACAAATT pLKO_005 2461 3UTR 100% 13.200 9.240 N CDK18 n/a
4 TRCN0000350588 GAACCTGAAGCACGCCAATAT pLKO_005 892 3UTR 100% 13.200 9.240 N CDK18 n/a
5 TRCN0000315411 GCATGCACAACGTCAAGATTT pLKO_005 1020 3UTR 100% 13.200 9.240 N CDK18 n/a
6 TRCN0000381363 ACAGTGACCTGAAGCAGTATC pLKO_005 975 3UTR 100% 10.800 7.560 N CDK18 n/a
7 TRCN0000379497 ATGCACACGGATGACAGAATC pLKO_005 2743 3UTR 100% 10.800 7.560 N CDK18 n/a
8 TRCN0000195355 CATACGTGAAACTGGACAAAC pLKO.1 735 3UTR 100% 10.800 7.560 N CDK18 n/a
9 TRCN0000381142 GCTCTCTCTGCCCATGGATAT pLKO_005 595 3UTR 100% 10.800 7.560 N CDK18 n/a
10 TRCN0000380996 TGGAGCCCACACACGTTTCAT pLKO_005 2862 3UTR 100% 5.625 3.938 N CDK18 n/a
11 TRCN0000199410 CCACGGCTGTTTCTTCTTTGT pLKO.1 2570 3UTR 100% 4.950 3.465 N CDK18 n/a
12 TRCN0000006336 CGCACTGAGACCATTGAAGAA pLKO.1 362 3UTR 100% 4.950 3.465 N CDK18 n/a
13 TRCN0000006335 GAAACTGGAAACATACGTGAA pLKO.1 724 3UTR 100% 4.050 2.835 N CDK18 n/a
14 TRCN0000315409 GAAACTGGAAACATACGTGAA pLKO_005 724 3UTR 100% 4.050 2.835 N CDK18 n/a
15 TRCN0000199950 GAACCTCATGAGCATGCACAA pLKO.1 1009 3UTR 100% 4.050 2.835 N CDK18 n/a
16 TRCN0000006337 GCGCAGCAAACTGACGGAGAA pLKO.1 790 3UTR 100% 1.350 0.945 N CDK18 n/a
17 TRCN0000199711 GCACCTCATCTTTCGCCTCCT pLKO.1 1357 3UTR 100% 0.720 0.504 N CDK18 n/a
18 TRCN0000199592 GCGCCGTTTCTCCCTGTCAGT pLKO.1 337 3UTR 100% 0.000 0.000 N CDK18 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001737213.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491649 GGCGACGAAAAACCGGATATGAGT pLX_317 14.6% 53.8% V5 (not translated due to prior stop codon) 1_307del;580_581ins90;2425_3842delinsG n/a
2 ccsbBroadEn_14732 pDONR223 0% 36.8% None (many diffs) n/a
3 ccsbBroad304_14732 pLX_304 0% 36.8% V5 (many diffs) n/a
4 TRCN0000466439 TTTGATTTCATTTCTTTCGAACAA pLX_317 22.4% 36.8% V5 (many diffs) n/a
5 TRCN0000488438 TGGGCAGAAAGGATGCTTCTAAAC pLX_317 22.5% 36.8% V5 (not translated due to prior stop codon) (many diffs) n/a
6 ccsbBroadEn_10440 pDONR223 100% 36.7% None (many diffs) n/a
7 ccsbBroad304_10440 pLX_304 0% 36.7% V5 (many diffs) n/a
8 TRCN0000467401 ATTCAGTCAAGCCTAAACTATCTA pLX_317 26.3% 36.7% V5 (many diffs) n/a
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