Construct: ORF TRCN0000491671
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013388.1_s317c1
- Derived from:
- ccsbBroadEn_06086
- DNA Barcode:
- CGGCAGCGCTTACGCACGCAAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCTN1 (1639)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491671
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1639 | DCTN1 | dynactin subunit 1 | NM_001135041.2 | 99.9% | 99.7% | 1174C>A;1451A>G;3404G>T |
| 2 | human | 1639 | DCTN1 | dynactin subunit 1 | NM_023019.3 | 99.4% | 99.3% | (many diffs) |
| 3 | human | 1639 | DCTN1 | dynactin subunit 1 | NM_001190836.1 | 91.7% | 90.7% | (many diffs) |
| 4 | human | 1639 | DCTN1 | dynactin subunit 1 | NM_001135040.2 | 90.5% | 89.4% | (many diffs) |
| 5 | human | 1639 | DCTN1 | dynactin subunit 1 | NM_001190837.1 | 89.4% | 89% | (many diffs) |
| 6 | human | 1639 | DCTN1 | dynactin subunit 1 | NM_004082.4 | 88.9% | 88.5% | (many diffs) |
| 7 | human | 1639 | DCTN1 | dynactin subunit 1 | NR_033935.1 | 76.1% | (many diffs) | |
| 8 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505495.4 | 89.5% | 96.9% | (many diffs) |
| 9 | mouse | 13191 | Dctn1 | dynactin 1 | NM_001347310.1 | 89.2% | 96.5% | (many diffs) |
| 10 | mouse | 13191 | Dctn1 | dynactin 1 | XM_030255136.1 | 81.8% | 88.2% | (many diffs) |
| 11 | mouse | 13191 | Dctn1 | dynactin 1 | NM_001198867.1 | 81.8% | 88% | (many diffs) |
| 12 | mouse | 13191 | Dctn1 | dynactin 1 | XM_030255137.1 | 81.8% | 88% | (many diffs) |
| 13 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505492.2 | 81.6% | 87.9% | (many diffs) |
| 14 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505493.2 | 81.5% | 87.7% | (many diffs) |
| 15 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505494.1 | 81.5% | 87.7% | (many diffs) |
| 16 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505490.2 | 81.2% | 87.4% | (many diffs) |
| 17 | mouse | 13191 | Dctn1 | dynactin 1 | NM_001198866.1 | 80.8% | 87.2% | (many diffs) |
| 18 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505488.3 | 80.8% | 87.2% | (many diffs) |
| 19 | mouse | 13191 | Dctn1 | dynactin 1 | XM_030255135.1 | 80.8% | 87.2% | (many diffs) |
| 20 | mouse | 13191 | Dctn1 | dynactin 1 | XM_017321379.2 | 80.7% | 87% | (many diffs) |
| 21 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505491.4 | 80.7% | 86.8% | (many diffs) |
| 22 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505487.3 | 80.5% | 86.7% | (many diffs) |
| 23 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505489.3 | 80.4% | 86.5% | (many diffs) |
| 24 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505486.4 | 80.1% | 86.2% | (many diffs) |
| 25 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505485.2 | 80% | 86.4% | (many diffs) |
| 26 | mouse | 13191 | Dctn1 | dynactin 1 | NM_007835.2 | 79.8% | 86.1% | (many diffs) |
| 27 | mouse | 13191 | Dctn1 | dynactin 1 | XM_006505496.4 | 66.9% | 71.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3483
- ORF length:
- 3417
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gagacaggca ccgacagccc gaaagaccac aactcggcga cccaagccca 121 cgcgcccagc cagtactggg gtggctgggg ccagtagctc cctgggcccc tctggctcag 181 cgtcagcagg tgagctgagc agcagtgagc ccagcacccc ggctcagact ccgctggcag 241 cacccatcat ccccacgccg gtcctcacct ctcctggagc agtccccccg cttccttccc 301 catccaagga ggaggaggga ctaagggctc aggtgcggga cctggaggag aaactagaga 361 ccctgagact gaaacgggca gaagacaaag caaagctaaa agagctggag aaacacaaaa 421 tccagctgga gcaggtgcag gaatggaaga gcaaaatgca ggagcagcag gccgacctgc 481 agcggcgcct caaggaggcg agaaaggaag ccaaggaggc gctggaggca aaggaacgct 541 atatggagga gatggctgat actgctgatg ccattgagat ggccactttg gacaaggaga 601 tggctgaaga gcgggctgag tccctgcagc aggaggtgga ggcactgaag gagcgggtgg 661 acgagctcac tactgactta gagatcctca aggctgagat tgaagagaag ggctcagatg 721 gcgctgcatc cagttatcag ctcaagcagc ttgaggagca gaatgcccgc ctgaaggatg 781 ccctggtgag gatgcgggat ctttcttcct cagagaagca ggagcatgtg aagctccaga 841 agctcatgga aaagaagaac caagagctgg aagttgtgag gcaacagcgg gagcgtctgc 901 aggaggagct aagccaggca gagagcacca ttgatgagct caaggagcag gtggatgctg 961 ctctgggtgc tgaggagatg gtggagatgc tgacagatcg gaacctgaat ctggaagaga 1021 aagtgcgcga gttgagggag actgtgggag acttggaagc gatgaatgag atgaacgatg 1081 agctgcagga gaatgcacgt gagacagaac tggagctgcg ggagcagctg gacatggcag 1141 gcgcgcgggt tcgtgaggcc cagaagcgtg tggaggcagc ccaggagacg gttgcagact 1201 accagcagac catcaagaag taccgccagc tgaccgccaa tctacaggat gtgaatcggg 1261 aactgacaaa ccagcaggaa gcatctgtgg agaggcaaca gcagccacct ccagagacct 1321 ttgacttcaa aatcaagttt gctgagacta aggcccatgc caaggcaatt gagatggaat 1381 tgaggcagat ggaggtggcc caggccaatc gacacatgtc cctgctgaca gccttcatgc 1441 ctgacagctt ccttcggcca ggtggggacc atgactgcgt tctggtgctg ttgctcatgc 1501 ctcgtctcat ttgcagggca gagctgatcc ggaagcaggc ccaggagaag tttgaactaa 1561 gtgagaactg ttcagagcgg cctgggctgc gaggagctgc tggggagcaa ctcagctttg 1621 ctgctggact ggtgtactcg ctgagcctgc tgcaggccac gctacaccgc tatgagcatg 1681 ccctctctca gtgcagtgtg gatgtgtata agaaagtggg cagcctgtac cctgagatga 1741 gtgcccatga gcgctccttg gatttcctca ttgaactgct gcacaaggat cagctggatg 1801 agactgtcaa tgtggagcct ctcaccaagg ccatcaagta ctatcagcat ctgtacagca 1861 tccaccttgc cgaacagcct gaggactgta ctatgcagct ggctgaccac attaagttca 1921 cgcagagtgc tctggactgc atgagtgtgg aggtaggacg gctgcgtgcc ttcttgcagg 1981 gtgggcagga ggctacagat attgccctcc tgctccggga tctggaaact tcatgcagtg 2041 acatccgcca gttctgcaag aagatccgaa ggcgaatgcc agggacagat gctcctggga 2101 tcccagctgc actggccttt ggaccacagg tatctgacac gctcctagac tgcaggaaac 2161 acttgacgtg ggtcgtggct gtgctgcagg aggtggcagc tgctgctgcc cagctcattg 2221 ccccactggc agagaatgag gggctacttg tggctgctct ggaggaactg gctttcaaag 2281 caagcgagca gatctatggg accccctcca gcagccccta tgagtgtctg cgccagtcat 2341 gcaacatcct catcagtacc atgaacaagc tggccacagc catgcaggag ggggagtatg 2401 atgcagagcg gccccccagc aagcctccac cggttgaact gcgggctgct gcccttcgtg 2461 cagagatcac agatgctgaa ggcctgggtt tgaagctcga agatcgagag acagttatta 2521 aggagttgaa gaagtcactc aagattaagg gagaggagct aagtgaggcc aatgtgcggc 2581 tgagcctcct ggagaagaag ttggacagtg ctgccaagga tgcagatgag cgcatcgaga 2641 aagtccagac tcggctggag gagacccagg cactgctgcg aaagaaggag aaagagtttg 2701 aggagacaat ggatgcactc caggctgaca tcgaccagct ggaggcagag aaggcagaac 2761 taaagcagcg tctgaacagc cagtccaaac gcacgattga gggactccgg ggccctcctc 2821 cttcaggcat tgctactctg gtctctggca ttgctggtgg agccatccct gggcaggctc 2881 cagggtctgt gccaggccca gggctggtga aggactcacc actgctgctt cagcagatct 2941 ctgccatgag gctgcacatc tcccagctcc agcatgagaa cagcatcctc aagggagccc 3001 agatgaaggc atccttggca tccctgcccc ctctgcatgt tgcaaagcta tcccatgagg 3061 gccctggcag tgagttacca gctggagcgc tgtatcgtaa gaccagccag ctgctggaga 3121 cattgaatca attgagcaca cacacgcacg tagtagacat cactcgcacc agccctgctg 3181 ccaagagccc gtcggcccaa cttatggagc aagtggcTCA GCTTAAGTCC CTGAGTGACA 3241 CCGTCGAGAA GCTCAAGGAT GAGGTCCTCA AGGAGACAGT ATCTCAGCGC CCTGGAGCCA 3301 CAGTACCCAC TGACTTTGCC ACCTTCCCTT CATCAGCCTT CCTCAGGGCC AAGGAGGAGC 3361 AGCAGGATGA CACAGTCTAC ATGGGCAAAG TGACCTTCTC ATGTGCGGCT GGTTTTGGAC 3421 AGCGACACCG GCTGGTGCTG ACCCAGGAGC AGCTGCACCA GCTTCACATT CGCCTCATCT 3481 CCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 3541 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 3601 GGCTTTATAT ATCTTGTGGA AAGGACGACG GCAGCGCTTA CGCACGCAAG AAACGCGTTA 3661 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt