Construct: ORF TRCN0000491679
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000028.1_s317c1
- Derived from:
- ccsbBroadEn_05268
- DNA Barcode:
- ACAGCACTAGCAGGAACCTGGACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMED4 (222068)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491679
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222068 | TMED4 | transmembrane p24 trafficki... | NM_182547.4 | 99.8% | 100% | 390G>T |
| 2 | human | 222068 | TMED4 | transmembrane p24 trafficki... | NM_001303058.1 | 80% | 79.2% | (many diffs) |
| 3 | human | 222068 | TMED4 | transmembrane p24 trafficki... | NM_001303059.2 | 78.4% | 78.4% | 386_387ins147 |
| 4 | human | 222068 | TMED4 | transmembrane p24 trafficki... | NM_001303061.2 | 73.8% | 74% | 0_1ins177;213G>T |
| 5 | human | 222068 | TMED4 | transmembrane p24 trafficki... | NM_001303062.2 | 73.8% | 74% | 0_1ins177;213G>T |
| 6 | human | 222068 | TMED4 | transmembrane p24 trafficki... | NM_001303060.1 | 54.2% | 53.3% | (many diffs) |
| 7 | mouse | 103694 | Tmed4 | transmembrane p24 trafficki... | NM_134020.1 | 87.1% | 94.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 747
- ORF length:
- 681
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aggtgtcggg gctgggcctc tgcgggcgat ggggcggcag gccctgctgc 121 ttctcgcgct gtgcgccaca ggcgcccagg ggctctactt ccacatcggc gagaccgaga 181 agcgctgttt catcgaggaa atccccgacg agaccatggt catcggcaac tatcgtaccc 241 agatgtggga taagcagaag gaggtcttcc tgccctcgac ccctggcctg ggcatgcacg 301 tggaagtgaa ggaccccgac ggcaaggtgg tgctgtcccg gcagtacggc tcggagggcc 361 gcttcacgtt cacctcccac acgcccggtg accatcaaat ctgtctgcac tccaattcta 421 ccaggatggc tctcTTCGCT GGTGGCAAAC TGCGTGTGCA TCTCGACATC CAGGTTGGGG 481 AGCATGCCAA CAACTACCCT GAGATTGCTG CAAAAGATAA GCTGACGGAG CTACAGCTCC 541 GCGCCCGCCA GTTGCTTGAT CAGGTGGAAC AGATTCAGAA GGAGCAGGAT TACCAAAGGT 601 ATCGTGAAGA GCGCTTCCGA CTGACGAGCG AGAGCACCAA CCAGAGGGTC CTATGGTGGT 661 CCATTGCTCA GACTGTCATC CTCATCCTCA CTGGCATCTG GCAGATGCGT CACCTCAAGA 721 GCTTCTTTGA GGCCAAGAAG CTGGTGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 781 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 841 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACAGCACT 901 AGCAGGAACC TGGACTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 961 aagatt