Construct: ORF TRCN0000491686
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015976.1_s317c1
- Derived from:
- ccsbBroadEn_15760
- DNA Barcode:
- CACATAGGCCCCTATATTCGGAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ITGB3BP (23421)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491686
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23421 | ITGB3BP | integrin subunit beta 3 bin... | NM_001347148.1 | 99.7% | 97% | 395delT |
| 2 | human | 23421 | ITGB3BP | integrin subunit beta 3 bin... | XM_017000854.2 | 87.8% | 85.6% | (many diffs) |
| 3 | human | 23421 | ITGB3BP | integrin subunit beta 3 bin... | XM_024446042.1 | 87.8% | 85.6% | (many diffs) |
| 4 | human | 23421 | ITGB3BP | integrin subunit beta 3 bin... | NM_014288.4 | 77.2% | 75.1% | 1_120del;515delT |
| 5 | human | 23421 | ITGB3BP | integrin subunit beta 3 bin... | NM_001347145.1 | 71.6% | 66.1% | (many diffs) |
| 6 | human | 23421 | ITGB3BP | integrin subunit beta 3 bin... | NM_001347147.1 | 71.4% | 70.2% | (many diffs) |
| 7 | human | 23421 | ITGB3BP | integrin subunit beta 3 bin... | XM_017000853.2 | 69.2% | 67.2% | (many diffs) |
| 8 | human | 23421 | ITGB3BP | integrin subunit beta 3 bin... | XM_017000855.2 | 64.7% | 63% | 1_222del;617delT |
| 9 | human | 23421 | ITGB3BP | integrin subunit beta 3 bin... | NM_001206739.1 | 63.2% | 61.5% | 1_237del;632delT |
| 10 | human | 23421 | ITGB3BP | integrin subunit beta 3 bin... | XM_024446041.1 | 58.6% | 56.8% | (many diffs) |
| 11 | human | 23421 | ITGB3BP | integrin subunit beta 3 bin... | NR_045147.1 | 38.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tctatttgct tctcccacaa gttctgaaga gcaaaagcac agaaatggac 121 tatcaaatga aaagagaaaa aaattgaatc acccCAGTTT AACTGAAAGC AAAGAATCTA 181 CAACAAAAGA CAATGATGAA TTCATGATGT TGCTATCAAA AGTTGAGAAA TTGTCAGAAG 241 AAATCATGGA GATAATGCAA AATTTAAGTA GTATACAGGC TTTGGAGGGC AGTAGAGAGC 301 TTGAAAATCT CATTGGAATC TCCTGTGCAT CACATTTCTT AAAAAGAGAA ATGCAGAAAA 361 CCAAAGAACT AATGACAAAA GTGAATAAAC AAAAACTGTT TGAAAAGAGT ACAGGACTTC 421 CTCACAAAGC ATCACGTCAT CTTGACAGCT ATGAATTCCT AAAGCCATTT TAAACTGCCC 481 AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT 541 CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA 601 TATATCTTGT GGAAAGGACG ACACATAGGC CCCTATATTC GGAATACGCG TTAAGTCgac 661 aatcaacctc tggattacaa aatttgtgaa agatt