Transcript: Human XM_017000855.2

PREDICTED: Homo sapiens integrin subunit beta 3 binding protein (ITGB3BP), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ITGB3BP (23421)
Length:
1008
CDS:
38..673

Additional Resources:

NCBI RefSeq record:
XM_017000855.2
NBCI Gene record:
ITGB3BP (23421)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017000855.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000057733 CCCAGTAAATACCAGCTCGTA pLKO.1 767 3UTR 100% 2.640 3.696 N ITGB3BP n/a
2 TRCN0000289038 CCCAGTAAATACCAGCTCGTA pLKO_005 767 3UTR 100% 2.640 3.696 N ITGB3BP n/a
3 TRCN0000296131 GTACAGGACTTCCTCACAAAG pLKO_005 603 CDS 100% 10.800 8.640 N ITGB3BP n/a
4 TRCN0000296109 ACTTGTCAAATGAGTCTATTT pLKO_005 251 CDS 100% 13.200 9.240 N ITGB3BP n/a
5 TRCN0000057735 CGTCATCTTGACAGCTATGAA pLKO.1 629 CDS 100% 5.625 3.938 N ITGB3BP n/a
6 TRCN0000289036 CGTCATCTTGACAGCTATGAA pLKO_005 629 CDS 100% 5.625 3.938 N ITGB3BP n/a
7 TRCN0000057737 GCACAGAAATGGACTATCAAA pLKO.1 301 CDS 100% 5.625 3.938 N ITGB3BP n/a
8 TRCN0000289037 GCACAGAAATGGACTATCAAA pLKO_005 301 CDS 100% 5.625 3.938 N ITGB3BP n/a
9 TRCN0000057734 CCCAGTTTAACTGAAAGCAAA pLKO.1 347 CDS 100% 4.950 3.465 N ITGB3BP n/a
10 TRCN0000057736 CCCACAAGTTCTGAAGAGCAA pLKO.1 278 CDS 100% 2.640 1.848 N ITGB3BP n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017000855.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02763 pDONR223 100% 80.1% 73.6% None (many diffs) n/a
2 ccsbBroad304_02763 pLX_304 0% 80.1% 73.6% V5 (many diffs) n/a
3 ccsbBroadEn_15760 pDONR223 0% 64.9% 64.9% None 1_222del n/a
4 ccsbBroad304_15760 pLX_304 0% 64.9% 64.9% V5 1_222del n/a
5 TRCN0000491686 CACATAGGCCCCTATATTCGGAAT pLX_317 77.2% 64.7% 63% V5 (not translated due to prior stop codon) 1_222del;617delT n/a
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