Construct: ORF TRCN0000491697
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009517.2_s317c1
- Derived from:
- ccsbBroadEn_05603
- DNA Barcode:
- GATACGTCATTCGAAATCCTGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIN28B (389421)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491697
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389421 | LIN28B | lin-28 homolog B | NM_001004317.4 | 100% | 100% | |
| 2 | human | 389421 | LIN28B | lin-28 homolog B | XM_011535818.3 | 96.5% | 96.1% | 1_23del;26_27delCAinsG;31_32delCCinsGA |
| 3 | human | 389421 | LIN28B | lin-28 homolog B | XM_006715477.2 | 92.5% | 92.1% | 1_56del;59_60delCAinsG;64_65delCCinsGA |
| 4 | mouse | 380669 | Lin28b | lin-28 homolog B (C. elegans) | NM_001031772.2 | 77.7% | 78.1% | (many diffs) |
| 5 | mouse | 380669 | Lin28b | lin-28 homolog B (C. elegans) | XM_011243188.1 | 77.7% | 78.1% | (many diffs) |
| 6 | mouse | 380669 | Lin28b | lin-28 homolog B (C. elegans) | XM_011243187.2 | 74.9% | 74.9% | (many diffs) |
| 7 | mouse | 380669 | Lin28b | lin-28 homolog B (C. elegans) | XM_011243183.2 | 74.3% | 74.2% | (many diffs) |
| 8 | mouse | 380669 | Lin28b | lin-28 homolog B (C. elegans) | XM_011243185.2 | 74.3% | 74.2% | (many diffs) |
| 9 | mouse | 380669 | Lin28b | lin-28 homolog B (C. elegans) | XM_011243186.2 | 74.3% | 74.2% | (many diffs) |
| 10 | mouse | 380669 | Lin28b | lin-28 homolog B (C. elegans) | XM_011243182.2 | 70.9% | 70.9% | (many diffs) |
| 11 | mouse | 380669 | Lin28b | lin-28 homolog B (C. elegans) | XM_011243180.2 | 69.3% | 69.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 819
- ORF length:
- 750
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgaaggc ggggctagca aaggtggtgg agaagagccc gggaagctgc 121 cggagccggc agaggaggaa tcccaggttt tgcgcggaac tggccactgt aagtggttca 181 atgtgcgcat gggatttgga ttcatctcca tgataaaccg agagggaagc cccttggata 241 ttccagtcga tgtatttgta caccaaagca aactattcat ggaaggattt agaagcctaa 301 aagaaggaga accagtggaa ttcacattta aaaaatcttc caaaggcctt gagtcaatac 361 gggtaacagg acctggtggg agcccctgtt taggaagtga aagaagaccc aaagggaaga 421 cactacagaa aagaaaacca aagggagata gatgctacaa ctgtggtggc cttgatcatc 481 atgctaagga atgtagtcta cctccTCAGC CAAAGAAGTG CCATTACTGT CAGAGCATCA 541 TGCACATGGT GGCAAACTGC CCACATAAAA ATGTTGCACA GCCACCCGCG AGTTCTCAGG 601 GAAGACAGGA AGCAGAATCC CAGCCATGCA CTTCAACTCT CCCTCGAGAA GTGGGAGGCG 661 GGCATGGCTG TACATCACCA CCGTTTCCTC AGGAGGCTAG GGCAGAGATC TCAGAACGGT 721 CAGGCAGGTC ACCTCAAGAA GCTTCCTCCA CGAAGTCATC TATAGCACCA GAAGAGCAAA 781 GCAAAAAGGG GCCTTCAGTT CAAAAAAGGA AAAAGACATT GCCAACTTTC TTGTACAAAG 841 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 901 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 961 ACGAGATACG TCATTCGAAA TCCTGCAGAC GCGTTAAGTC gacaatcaac ctctggatta 1021 caaaatttgt gaaagatt