Construct: ORF ccsbBroadEn_05603

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009517.1_s300c1, BRDN0000455613
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LIN28B (389421)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 389421 LIN28B lin-28 homolog B NM_001004317.4 100% 100%
2 human 389421 LIN28B lin-28 homolog B XM_011535818.3 96.5% 96.1% 1_23del;26_27delCAinsG;31_32delCCinsGA
3 human 389421 LIN28B lin-28 homolog B XM_006715477.2 92.5% 92.1% 1_56del;59_60delCAinsG;64_65delCCinsGA
4 mouse 380669 Lin28b lin-28 homolog B (C. elegans) NM_001031772.2 77.7% 78.1% (many diffs)
5 mouse 380669 Lin28b lin-28 homolog B (C. elegans) XM_011243188.1 77.7% 78.1% (many diffs)
6 mouse 380669 Lin28b lin-28 homolog B (C. elegans) XM_011243187.2 74.9% 74.9% (many diffs)
7 mouse 380669 Lin28b lin-28 homolog B (C. elegans) XM_011243183.2 74.3% 74.2% (many diffs)
8 mouse 380669 Lin28b lin-28 homolog B (C. elegans) XM_011243185.2 74.3% 74.2% (many diffs)
9 mouse 380669 Lin28b lin-28 homolog B (C. elegans) XM_011243186.2 74.3% 74.2% (many diffs)
10 mouse 380669 Lin28b lin-28 homolog B (C. elegans) XM_011243182.2 70.9% 70.9% (many diffs)
11 mouse 380669 Lin28b lin-28 homolog B (C. elegans) XM_011243180.2 69.3% 69.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
819
ORF length:
750
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGCCGAAGGC GGGGCTAGCA AAGGTGGTGG AGAAGAGCCC GGGAAGCTGC
121CGGAGCCGGC AGAGGAGGAA TCCCAGGTTT TGCGCGGAAC TGGCCACTGT AAGTGGTTCA
181ATGTGCGCAT GGGATTTGGA TTCATCTCCA TGATAAACCG AGAGGGAAGC CCCTTGGATA
241TTCCAGTCGA TGTATTTGTA CACCAAAGCA AACTATTCAT GGAAGGATTT AGAAGCCTAA
301AAGAAGGAGA ACCAGTGGAA TTCACATTTA AAAAATCTTC CAAAGGCCTT GAGTCAATAC
361GGGTAACAGG ACCTGGTGGG AGCCCCTGTT TAGGAAGTGA AAGAAGACCC AAAGGGAAGA
421CACTACAGAA AAGAAAACCA AAGGGAGATA GATGCTACAA CTGTGGTGGC CTTGATCATC
481ATGCTAAGGA ATGTAGTCTA CCTCCTCAGC CAAAGAAGTG CCATTACTGT CAGAGCATCA
541TGCACATGGT GGCAAACTGC CCACATAAAA ATGTTGCACA GCCACCCGCG AGTTCTCAGG
601GAAGACAGGA AGCAGAATCC CAGCCATGCA CTTCAACTCT CCCTCGAGAA GTGGGAGGCG
661GGCATGGCTG TACATCACCA CCGTTTCCTC AGGAGGCTAG GGCAGAGATC TCAGAACGGT
721CAGGCAGGTC ACCTCAAGAA GCTTCCTCCA CGAAGTCATC TATAGCACCA GAAGAGCAAA
781GCAAAAAGGG GCCTTCAGTT CAAAAAAGGA AAAAGACATT GCCAACTTTC TTGTACAAAG
841TTGGcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

Download FASTA (ORF) (Full)