Transcript: Mouse NM_001031772.2

Mus musculus lin-28 homolog B (C. elegans) (Lin28b), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Lin28b (380669)
Length:
5420
CDS:
142..957

Additional Resources:

NCBI RefSeq record:
NM_001031772.2
NBCI Gene record:
Lin28b (380669)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001031772.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000124704 CCTCCCAAATATAGGATGTTT pLKO.1 4349 3UTR 100% 5.625 7.875 N Lin28b n/a
2 TRCN0000124705 GCCAGTGGAATTTACATTTAA pLKO.1 378 CDS 100% 15.000 10.500 N Lin28b n/a
3 TRCN0000219859 ACTATTCATGGAAGGATTTAG pLKO.1 339 CDS 100% 13.200 9.240 N LIN28B n/a
4 TRCN0000124706 CCTTTGATTCAGAAACGGAAA pLKO.1 928 CDS 100% 4.050 2.835 N Lin28b n/a
5 TRCN0000122599 GCCTTGAGTCAATACGGGTAA pLKO.1 413 CDS 100% 4.050 2.835 N LIN28B n/a
6 TRCN0000124707 CGGCAGGATTTACTGATGGAT pLKO.1 526 CDS 100% 3.000 2.100 N Lin28b n/a
7 TRCN0000124708 CCCTTGGATATTCCAGTGGAT pLKO.1 298 CDS 100% 2.640 1.848 N Lin28b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001031772.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05603 pDONR223 100% 77.7% 78.1% None (many diffs) n/a
2 ccsbBroad304_05603 pLX_304 0% 77.7% 78.1% V5 (many diffs) n/a
3 TRCN0000491697 GATACGTCATTCGAAATCCTGCAG pLX_317 41.9% 77.7% 78.1% V5 (many diffs) n/a
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