Construct: ORF TRCN0000491698
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009631.1_s317c1
- Derived from:
- ccsbBroadEn_11756
- DNA Barcode:
- TGACGGACTGTTAATTGCACCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLA2G15 (23659)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491698
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23659 | PLA2G15 | phospholipase A2 group XV | XM_011522980.3 | 75.8% | 78.1% | 1_95del;177_178ins125 |
| 2 | human | 23659 | PLA2G15 | phospholipase A2 group XV | NM_012320.4 | 65.9% | 66% | 1_420del;573C>T |
| 3 | human | 23659 | PLA2G15 | phospholipase A2 group XV | XM_011522979.2 | 61.1% | 61.2% | 1_516del;669C>T |
| 4 | human | 23659 | PLA2G15 | phospholipase A2 group XV | NM_001363551.2 | 23.3% | 7.7% | 1_420del;502_503ins125;708_709ins403 |
| 5 | mouse | 192654 | Pla2g15 | phospholipase A2, group XV | NM_133792.2 | 57% | 60.6% | (many diffs) |
| 6 | mouse | 192654 | Pla2g15 | phospholipase A2, group XV | XM_006530772.1 | 20.6% | 10.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 882
- ORF length:
- 816
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggagagcctt gtgggctggg gctacacacg gggtgaggat gtccgagggg 121 ctccctatga ctggcgccga gccccaaatg aaaacgggcc ctacttcctg gccctccgcg 181 agatgatcga ggagatgtac cagctgtatg ggggccctgt ggtgctggtt gcccacagta 241 tgggcaacat gtacacgctc tactttctgc agcggcagcc gcaggcctgg aaggacaagt 301 atatccgggc cttcgtgtca ctgggtgcgc cctggggggg cgtggccaag accctgcgcg 361 tcctggcttc aggagacaac aaccggatcc cagtcatcgg gcccctgaag atccgggagc 421 agcagcggtc agctgtctcc accagctggc tgctgcccta caactacaca tggtcacctg 481 agaaggtgtt cgtgcagaca cccacaatca actacacact gcgggactac cgcaagttcT 541 TCCAGGACAT CGGCTTTGAA GATGGCTGGC TCATGCGGCA GGACACAGAA GGGCTGGTGG 601 AAGCCACGAT GCCACCTGGC GTGCAGCTGC ACTGCCTCTA TGGTACTGGC GTCCCCACAC 661 CAGACTCCTT CTACTATGAG AGCTTCCCTG ACCGTGACCC TAAAATCTGC TTTGGTGACG 721 GCGATGGTAC TGTGAACTTG AAGAGTGCCC TGCAGTGCCA GGCCTGGCAG AGCCGCCAGG 781 AGCACCAAGT GTTGCTGCAG GAGCTGCCAG GCAGCGAGCA CATCGAGATG CTGGCCAACG 841 CCACCACCCT GGCCTATCTG AAACGTGTGC TCCTTGGGCC CTGCCCAACT TTCTTGTACA 901 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 961 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1021 AGGACGATGA CGGACTGTTA ATTGCACCTG CACGCGTTAA GTCgacaatc aacctctgga 1081 ttacaaaatt tgtgaaagat t