Construct: ORF TRCN0000491711
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002820.1_s317c1
- Derived from:
- ccsbBroadEn_08021
- DNA Barcode:
- ACGCAACTAACCGCTGTTCTTTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHIC2 (26511)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491711
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26511 | CHIC2 | cysteine rich hydrophobic d... | NM_012110.4 | 100% | 100% | |
| 2 | human | 26511 | CHIC2 | cysteine rich hydrophobic d... | XM_006714037.4 | 83% | 83% | 246_247ins84 |
| 3 | human | 26511 | CHIC2 | cysteine rich hydrophobic d... | XM_011534382.2 | 71.7% | 67.8% | (many diffs) |
| 4 | mouse | 74277 | Chic2 | cysteine-rich hydrophobic d... | NM_028850.5 | 95.9% | 99.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 561
- ORF length:
- 495
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggatttcgac gaaatctatg aggaagagga ggacgaggag cgggccctgg 121 aggagcagct gctcaagtac tcgccggacc cggtggtcgt ccgcggctcc ggtcacgtca 181 ccgtatttgg actgagcaac aaatttgaat ctgaattccc TTCTTCATTA ACTGGAAAAG 241 TAGCTCCTGA AGAATTTAAA GCCAGCATCA ACAGAGTTAA CAGTTGTCTT AAGAAGAACC 301 TTCCTGTTAA TGTACGTTGG CTACTTTGTG GCTGCCTTTG TTGCTGCTGC ACATTAGGTT 361 GCAGTATGTG GCCAGTTATT TGCCTCAGTA AAAGAACACG AAGATCGATT GAGAAGTTAT 421 TAGAATGGGA AAACAATAGG TTATACCACA AGCTGTGCTT GCATTGGAGA CTGAGCAAAA 481 GGAAATGTGA AACGAATAAC ATGATGGAAT ATGTCATCCT CATAGAATTT TTACCAAAGA 541 CACCGATTTT TCGACCAGAT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 601 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 661 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACGC AACTAACCGC 721 TGTTCTTTCG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt