Construct: ORF TRCN0000491725
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021571.2_s317c1
- DNA Barcode:
- TCATACATTCGTGTCTCGCGCAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PTPN18 (26469)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491725
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26469 | PTPN18 | protein tyrosine phosphatas... | NM_001142370.2 | 99.4% | 99.4% | 792_797delGACGGG |
| 2 | human | 26469 | PTPN18 | protein tyrosine phosphatas... | XM_006712416.4 | 98.3% | 98.3% | 792_797delGACGGG;1060_1071del |
| 3 | human | 26469 | PTPN18 | protein tyrosine phosphatas... | XM_017003798.1 | 80.3% | 80.3% | 0_1ins192;600_605delGACGGG;868_879del |
| 4 | human | 26469 | PTPN18 | protein tyrosine phosphatas... | NM_014369.4 | 76.3% | 76.3% | 94_414del;1113_1118delGACGGG |
| 5 | human | 26469 | PTPN18 | protein tyrosine phosphatas... | XM_006712417.1 | 75.6% | 75.6% | 94_414del;1113_1118delGACGGG;1381_1392del |
| 6 | human | 26469 | PTPN18 | protein tyrosine phosphatas... | XM_024452789.1 | 70.2% | 70.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1125
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagccgc agcctggact cggcgcggag cttcctggag cggctggaag 121 cgcggggcgg ccgggagggg gcagtcctcg ccggcgagtt cagcaaaagg tgtgagcggt 181 actgggccca ggagcaggag ccactgcaga ctgggctttt ctgcatcact ctgataaagg 241 agaagtggct gaatgaggac atcatgctca ggaccctcaa ggtcacattc cagaaggagt 301 cccgttctgt gtaccagcta cagtatatgt cctggccaga ccgtggggtc cccagcagtc 361 ctgaccacat gctcgccatg gtggaggaag cccgtcgcct ccagggatct ggccctgaac 421 ccctctgtgt ccactgcagt gcgggttgtg ggcgaacagg cgtcctgtgc accgtggatt 481 atgtgaggca gctgctcctg acccagatga tcccacctga cttcagtctc tttgatgtgg 541 tccttaagat gaggaagcag cggcctgcgg ccgtgcagac agaggagcag tacaggttcc 601 tgtaccacac ggtggctcag atgttctgct ccacactcca gaatgccagc ccccactacc 661 agaacatcaa agagaattgt gccccactct acgacgatgc cctcttcctc cggactcccc 721 aggcacttct cgccataccc cgcccaccag gaggggtcct caggagcatc tctgtgcccg 781 ggtccccggg ccacgccatg gctgacaccT ACGCGGTGGT GCAGAAGCGC GGGGCTCCAG 841 CGGGCGCCGG GAGTGGGACG CAGACGGGGA CGGGGACGGG GGCGCGCAGC GCGGAGGAGG 901 CGCCGCTCTA CAGCAAGGTG ACGCCGCGCG CCCAGCGACC CGGGGCGCAC GCGGAGGACG 961 CGAGGGGGAC GCTGCCTGGC CGCGTTCCTG CTGACCAAAG TCCTGCCGGA TCTGGCGCCT 1021 ACGAGGACGT GGCGGGTGGA GCTCAGACCG GTGGGCTAGG TTTCAACCTG CGCATTGGGA 1081 GGCCGAAGGG TCCCCGGGAC CCGCCTGCTG AGTGGACCCG GGTGTAGAAC CCAGCTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGATCATACA TTCGTGTCTC GCGCAGCACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt