Construct: ORF TRCN0000491751
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001698.1_s317c1
- Derived from:
- ccsbBroadEn_09760
- DNA Barcode:
- GCACTAATGCGGACGTACTGATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAW1 (164781)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491751
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 164781 | DAW1 | dynein assembly factor with... | NM_178821.3 | 99.9% | 99.7% | 1236G>C |
| 2 | human | 164781 | DAW1 | dynein assembly factor with... | NM_001330004.1 | 96.3% | 96.1% | 0_1ins45;1191G>C |
| 3 | human | 164781 | DAW1 | dynein assembly factor with... | NR_138459.1 | 57.3% | (many diffs) | |
| 4 | human | 164781 | DAW1 | dynein assembly factor with... | XM_017003490.1 | 52.6% | 52.5% | 0_1ins588;648G>C |
| 5 | mouse | 71227 | Daw1 | dynein assembly factor with... | XM_006496548.2 | 84.9% | 89.6% | (many diffs) |
| 6 | mouse | 71227 | Daw1 | dynein assembly factor with... | XM_006496547.2 | 82.4% | 86.9% | (many diffs) |
| 7 | mouse | 71227 | Daw1 | dynein assembly factor with... | XM_011238711.2 | 81.7% | 86% | (many diffs) |
| 8 | mouse | 71227 | Daw1 | dynein assembly factor with... | XM_011238710.2 | 79.3% | 83.4% | (many diffs) |
| 9 | mouse | 71227 | Daw1 | dynein assembly factor with... | NM_027725.3 | 63.9% | 66% | (many diffs) |
| 10 | mouse | 71227 | Daw1 | dynein assembly factor with... | XM_006496549.2 | 62% | 64% | (many diffs) |
| 11 | mouse | 71227 | Daw1 | dynein assembly factor with... | NR_027636.1 | 61.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1311
- ORF length:
- 1245
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gctcaagagc ctcctgctcc ggtattaccc gccaggaatt atgttggaat 121 atgaaaaaca tggagaatta aagactaagt ccatagattt gcttgatctt ggtcccagca 181 ctgatgtcag tgcgttagta gaagaaatcc agaaggcaga acctctactc acagcttcac 241 gaacagagca agtcaaactt ttgatacaga ggttgcaaga gaaactcggc cagaacagca 301 atcacacgtt ctatcttttt aaggttctca aagcacatat attgccactg actaatgttg 361 cacttaacaa atcgggctca tgctttatca caggaagcta tgatcggacg tgcaagctct 421 gggacactgc gtctggagag gagctgaaca cgctggaggg ccacaggaat gtggtttatg 481 ccatagcatt caacaatcct tacggtgaca aaatcgccac tgggtccttt gataaaactt 541 gtaaactctg gagtgtggaa acaggaaaat gttaccatac cttcaggggt catacagcag 601 aaatagtgtg tttatcattt aaccctcaaa gcacattggt ggcgactgga agtatggaca 661 caacagccaa attgtgggac attcagaatg gcgaggaagt ttacacctta agaggacatt 721 ctgccgaaat catctccttg tcatttaaca cctcaggaga cagaatcatc acggggtctt 781 ttgatcatac cgttgtagtg tgggacgctg atactggaag gaaggtaaat atcttaattg 841 gtcattgtgc tgagattagc agtgcctcat tcaattggga ttGCTCTCTA ATATTAACTG 901 GCTCTATGGA CAAAACCTGC AAGCTGTGGG ATGCTACAAA TGGAAAATGT GTGGCAACCT 961 TAACAGGCCA TGATGATGAA ATACTAGACA GCTGCTTTGA TTACACTGGA AAGCTTATTG 1021 CAACTGCTTC AGCTGATGGA ACAGCAAGAA TTTTCAGTGC TGCCACAAGA AAATGCATTG 1081 CCAAACTGGA AGGTCATGAA GGTGAAATTT CAAAGATTTC TTTCAACCCT CAAGGGAACC 1141 ATCTTCTAAC TGGCAGCTCT GACAAAACGG CTAGAATCTG GGATGCTCAG ACTGGCCAGT 1201 GCCTCCAGGT TCTTGAGGGG CACACTGATG AAATCTTTTC ATGTGCTTTC AACTATAAAG 1261 GCAACATAGT CATTACAGGC AGCAAGGATA ATACCTGTAG CATATGGCGT TGCCCAACTT 1321 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1381 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC ATTTCTTGGC TTTATATATC 1441 TTGTGGAAAG GACGAGCACT AATGCGGACG TACTGATTCA CGCGTTAAGT Cgacaatcaa 1501 cctctggatt acaaaatttg tgaaagatt