Transcript: Human NR_138459.1

Homo sapiens dynein assembly factor with WD repeats 1 (DAW1), transcript variant 3, non-coding RNA.

Source:
NCBI, updated 2018-06-03
Taxon:
Homo sapiens (human)
Gene:
DAW1 (164781)
Length:
2169
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_138459.1
NBCI Gene record:
DAW1 (164781)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_138459.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000077979 CCAGGAATTATGTTGGAATAT pLKO.1 351 3UTR 100% 13.200 18.480 N DAW1 n/a
2 TRCN0000435074 GCGAGGAAGTTTACACCTTAA pLKO_005 940 3UTR 100% 10.800 15.120 N DAW1 n/a
3 TRCN0000077981 CGACTGGAAGTATGGACACAA pLKO.1 892 3UTR 100% 4.950 6.930 N DAW1 n/a
4 TRCN0000421372 AGTTATGCCACTCCAATATTA pLKO_005 1945 3UTR 100% 15.000 12.000 N DAW1 n/a
5 TRCN0000430777 TTCACAGATATGACCATTAAA pLKO_005 1916 3UTR 100% 15.000 12.000 N DAW1 n/a
6 TRCN0000077978 GCAAGTCAACTATTTCTACAA pLKO.1 1884 3UTR 100% 4.950 3.465 N DAW1 n/a
7 TRCN0000077982 GCAGCAAGGATAATACCTGTA pLKO.1 1744 3UTR 100% 4.050 2.835 N DAW1 n/a
8 TRCN0000077980 GCTGCTTTGATTACACTGGAA pLKO.1 1240 3UTR 100% 2.640 1.848 N DAW1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_138459.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09760 pDONR223 100% 57.3% None (many diffs) n/a
2 ccsbBroad304_09760 pLX_304 0% 57.3% V5 (many diffs) n/a
3 TRCN0000491751 GCACTAATGCGGACGTACTGATTC pLX_317 34.7% 57.3% V5 (many diffs) n/a
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