Construct: ORF TRCN0000491774
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000987.2_s317c1
- Derived from:
- ccsbBroadEn_10807
- DNA Barcode:
- TGGTTTATTTCGGACTCGCAGGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ESR2 (2100)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491774
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2100 | ESR2 | estrogen receptor 2 | NM_001271877.1 | 72.8% | 72.6% | (many diffs) |
| 2 | human | 2100 | ESR2 | estrogen receptor 2 | NM_001271876.1 | 67.7% | 67% | (many diffs) |
| 3 | human | 2100 | ESR2 | estrogen receptor 2 | NM_001214902.1 | 66.8% | 66.1% | (many diffs) |
| 4 | human | 2100 | ESR2 | estrogen receptor 2 | NM_001040275.1 | 64.9% | 64.2% | (many diffs) |
| 5 | human | 2100 | ESR2 | estrogen receptor 2 | NM_001291712.2 | 64.9% | 64.2% | (many diffs) |
| 6 | human | 2100 | ESR2 | estrogen receptor 2 | NM_001291723.1 | 64.9% | 64.2% | (many diffs) |
| 7 | human | 2100 | ESR2 | estrogen receptor 2 | XM_017021084.1 | 64.9% | 64.2% | (many diffs) |
| 8 | human | 2100 | ESR2 | estrogen receptor 2 | NM_001437.2 | 60.6% | 60% | (many diffs) |
| 9 | human | 2100 | ESR2 | estrogen receptor 2 | XM_017021079.1 | 60.6% | 60% | (many diffs) |
| 10 | human | 2100 | ESR2 | estrogen receptor 2 | XM_017021080.1 | 60.6% | 60% | (many diffs) |
| 11 | human | 2100 | ESR2 | estrogen receptor 2 | XM_017021081.1 | 60.6% | 60% | (many diffs) |
| 12 | human | 2100 | ESR2 | estrogen receptor 2 | XM_017021082.1 | 60.6% | 60% | (many diffs) |
| 13 | human | 2100 | ESR2 | estrogen receptor 2 | XM_017021083.1 | 60.6% | 60% | (many diffs) |
| 14 | human | 2100 | ESR2 | estrogen receptor 2 | NR_073497.1 | 42.9% | (many diffs) | |
| 15 | human | 2100 | ESR2 | estrogen receptor 2 | XR_001750187.1 | 40% | 1_575del;1545_2417del | |
| 16 | human | 2100 | ESR2 | estrogen receptor 2 | NR_073496.2 | 32.5% | 1_806del;1776_2981del | |
| 17 | mouse | 13983 | Esr2 | estrogen receptor 2 (beta) | NM_010157.3 | 48.7% | 50.4% | (many diffs) |
| 18 | mouse | 13983 | Esr2 | estrogen receptor 2 (beta) | NM_207707.1 | 47.2% | 48.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1035
- ORF length:
- 969
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tataaaaaac tcaccatcta gccttaattc tccttcctcc tacaactgca 121 gtcaatccat cttacccctg gagcacggct ccatatacat accttcctcc tatgtagaca 181 gccaccatga atatccagcc atgacattct atagccctgc tgtgatgaat tacagcattc 241 ccagcaatgt cactaacttg gaaggtgggc ctggtcggca gaccacaagc ccaaatgtgt 301 tgtggccaac acctgggcac ctttctcctt tagtggtcca tcgccagtta tcacatctgt 361 atgcggaacc tcaaaagagt ccctggtgtg aagcaagatc gctagaacac accttacctg 421 taaacagaga gacactgaaa aggaaggtta gtgggaaccg ttgcgccagc cctgttactg 481 gtccaggttc aaagagggat gctcacttct gcgctgtctg cagcgattac gcatcgggat 541 atcactatgg agtctggtcg tgtgaaggat gtaaGGCCTT TTTTAAAAGA AGCATTCAAG 601 GACATAATGA TTATATTTGT CCAGCTACAA ATCAGTGTAC AATCGATAAA AACCGGCGCA 661 AGAGCTGCCA GGCCTGCCGA CTTCGGAAGT GTTACGAAGT GGGAATGGTG AAGTGTGGCT 721 CCCGGAGAGA GAGATGTGGG TACCGCCTTG TGCGGAGACA GAGAAGTGCC GACGAGCAGC 781 TGCACTGTGC CGGCAAGGCC AAGAGAAGTG GCGGCCACGC GCCCCGAGTG CGGGAGCTGC 841 TGCTGGACGC CCTGAGCCCC GAGCAGCTAG TGCTCACCCT CCTGGAGGCT GAGCCGCCCC 901 ATGTGCTGAT CAGCCGCCCC AGTGCGCCCT TCACCGAGGC CTCCATGATG ATGTCCCTGA 961 CCAAGTTGGC CGACAAGGAG TTGGTACACA TGATCAGCTG GGCCAAGAAG ATTCCCGGGA 1021 TGAGGGGAAA TGCGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGGTTTATTT CGGACTCGCA 1201 GGAGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt