Construct: ORF TRCN0000491796

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019964.2_s317c1
DNA Barcode:
TTGAAGTTAAAGATCCTGTCCCTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HNF4A (3172)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491796
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3172 HNF4A hepatocyte nuclear factor 4... NM_000457.4 99.9% 99.7% 1422_1423insG
2 human 3172 HNF4A hepatocyte nuclear factor 4... NM_178849.3 97.8% 97.4% 1252_1253ins30;1392_1393insG
3 human 3172 HNF4A hepatocyte nuclear factor 4... NM_175914.4 93.4% 92.6% (many diffs)
4 human 3172 HNF4A hepatocyte nuclear factor 4... NM_001287183.1 93.3% 92.2% (many diffs)
5 human 3172 HNF4A hepatocyte nuclear factor 4... NM_001258355.2 93.1% 90.7% (many diffs)
6 human 3172 HNF4A hepatocyte nuclear factor 4... XM_005260407.4 92.3% 92.2% 115_231del;1539_1540insG
7 human 3172 HNF4A hepatocyte nuclear factor 4... NM_001030003.3 91.3% 90.3% (many diffs)
8 human 3172 HNF4A hepatocyte nuclear factor 4... NM_001287182.2 91.2% 89.9% (many diffs)
9 human 3172 HNF4A hepatocyte nuclear factor 4... NM_178850.3 84.7% 79.3% (many diffs)
10 human 3172 HNF4A hepatocyte nuclear factor 4... NM_001030004.3 78.3% 72.4% (many diffs)
11 human 3172 HNF4A hepatocyte nuclear factor 4... NM_001287184.2 78.2% 72.1% (many diffs)
12 mouse 15378 Hnf4a hepatic nuclear factor 4, a... NM_008261.3 89.6% 95.5% (many diffs)
13 mouse 15378 Hnf4a hepatic nuclear factor 4, a... XM_006498787.1 87.6% 93.2% (many diffs)
14 mouse 15378 Hnf4a hepatic nuclear factor 4, a... XM_006498788.1 84.5% 88.6% (many diffs)
15 mouse 15378 Hnf4a hepatic nuclear factor 4, a... NM_001312906.1 83.8% 88.2% (many diffs)
16 mouse 15378 Hnf4a hepatic nuclear factor 4, a... NM_001312907.1 81.8% 85.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1497
ORF length:
1425
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgcgactc tccaaaaccc tcgtcgacat ggacatggcc gactacagtg
121ctgcactgga cccagcctac accaccctgg aatttgagaa tgtgcaggtg ttgacgatgg
181gcaatgacac gtccccatca gaaggcacca acctcaacgc gcccaacagc ctgggtgtca
241gcgccctgtg tgccatctgc ggggaccggg ccacgggcaa acactacggt gcctcgagct
301gtgacggctg caagggcttc ttccggagga gcgtgcggaa gaaccacatg tactcctgca
361gatttagccg gcagtgcgtg gtggacaaag acaagaggaa ccagtgccgc tactgcaggc
421tcaagaaatg cttccgggct ggcatgaaga aggaagccgt ccagaatgag cgggaccgga
481tcagcactcg aaggtcaagc tatgaggaca gcagcctgcc ctccatcaat gcgctcctgc
541aggcggaggt cctgtcccga cagatcacct cccccgtctc cgggatcaac ggcgacattc
601gggcgaagaa gattgccagc atcgcagatg tgtgtgagtc catgaaggag cagctgctgg
661ttctcgttga gtgggccaag tacatcccag ctttctgcga gctccccctg gacgaccagg
721tggccctgct cagagcccat gctggcgagc acctgctgct cggagccacc aagagatcca
781tggtgttcaa ggacgtgctg ctcctaggca atgactacat tgtccctcgg cactgcccgg
841agctggcgga gatgagccgg gtgtccatac gcatccttga cgagctggtg ctgcccttcc
901aggagctgca gatcgatgac aatgagtatg cctacctcaa agccatcatc ttctttgacc
961cagatgccaa ggggctgagc gatccaggga agatcaagcg gctgcgttcc caggtgcagg
1021tgagcttgga ggactacatc aacgaccgcc agtatgactc gcgtggccgc tttggagagc
1081tgctgctgct gctgcccacc ttgcagagca tcacctggca gatgatcgag cagatccagt
1141tcatcaagct cTTCGGCATG GCCAAGATTG ACAACCTGTT GCAGGAGATG CTGCTGGGAG
1201GGTCCCCCAG CGATGCACCC CATGCCCACC ACCCCCTGCA CCCTCACCTG ATGCAGGAAC
1261ATATGGGAAC CAACGTCATC GTTGCCAACA CAATGCCCAC TCACCTCAGC AACGGACAGA
1321TGTGTGAGTG GCCCCGACCC AGGGGACAGG CAGCCACCCC TGAGACCCCA CAGCCCTCAC
1381CGCCAGGTGG CTCAGGGTCT GAGCCCTATA AGCTCCTGCC GGGAGCCGTC GCCACAATCG
1441TCAAGCCCCT CTCTGCCATC CCCCAGCCGA CCATCACCAA GCAGGAAGTT ATCGACCCAG
1501CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1561ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1621TATCTTGTGG AAAGGACGAT TGAAGTTAAA GATCCTGTCC CTCACGCGTT AAGTCgacaa
1681tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)