Construct: ORF TRCN0000491798
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021669.2_s317c1
- DNA Barcode:
- CTTCTGGTCGTGTGGGCTGGGTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LCK (3932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491798
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | NM_001042771.2 | 94.4% | 94.4% | 963_964ins90 |
| 2 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | NM_005356.5 | 94.4% | 94.4% | 963_964ins90 |
| 3 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | XM_024447046.1 | 85.2% | 85.2% | 186_359del;1137_1138ins90 |
| 4 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | XM_024447047.1 | 85.2% | 85.2% | 186_359del;1137_1138ins90 |
| 5 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | NM_001330468.2 | 84.9% | 84.7% | 631_632ins153;810_811ins90 |
| 6 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | XM_011541453.3 | 76.7% | 76.5% | 186_359del;805_806ins153;984_985ins90 |
| 7 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | NM_001162433.1 | 83.7% | 91.4% | (many diffs) |
| 8 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | NM_010693.3 | 83.7% | 91.4% | (many diffs) |
| 9 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | XM_006502818.3 | 83.7% | 91.4% | (many diffs) |
| 10 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | XM_030253275.1 | 83.7% | 91.4% | (many diffs) |
| 11 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | XM_030253276.1 | 83.7% | 91.4% | (many diffs) |
| 12 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | NM_001162432.1 | 82% | 89.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1686
- ORF length:
- 1617
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gggctgtggc tgcagctcac acccggaaga tgactggatg gaaaacatcg 121 atgtgtgtga gaactgccat tatcccatag tcccactgga tggcaagggc acgctgctca 181 tccgaaatgg ctctgaggtg cgggacccac tggttaccta cgaaggctcc aatccgccgg 241 cttccccact gcaagacaac ctggttatcg ctctgcacag ctatgagccc tctcacgacg 301 gagatctggg ctttgagaag ggggaacagc tccgcatcct ggagcagagc ggcgagtggt 361 ggaaggcgca gtccctgacc acgggccagg aaggcttcat ccccttcaat tttgtggcca 421 aagcgaacag cctggagccc gaaccctggt tcttcaagaa cctgagccgc aaggacgcgg 481 agcggcagct cctggcgccc gggaacactc acggctcctt cctcatccgg gagagcgaga 541 gcaccgcggg atcgttttca ctgtcggtcc gggacttcga ccagaaccag ggagaggtgg 601 tgaaacatta caagatccgt aatctggaca acggtggctt ctacatctcc cctcgaatca 661 cttttcccgg cctgcatgaa ctggtccgcc attacaccaa tgcttcagat gggctgtgca 721 cacggttgag ccgcccctgc cagacccaga agccccagaa gccgtggtgg gaggacgagt 781 gggaggttcc cagggagacg ctgaagctgg tggagcggct gggggctgga cagttcgggg 841 aggtgtggat ggggtactac aacgggcaca cgaaggtggc ggtgaagagc ctgaagcagg 901 gcagcatgtc cccggacgcc ttcctggccg aggccaacct catgaagcag ctgcaacacc 961 agcggctggt tcggctctac gctgtggtca cccaggagcc catctacatc atcactgaat 1021 acatggagaa tgacacactt ctagactccc agttggagga gaaaggtctg ggggcctccc 1081 cctggggcaa cttgggccag caactcttgc ttctgcccac agggagtcta gtggattttc 1141 tcaagacccc ttcaggcatc aagttgacca tcaacaaact cctggacatg gcagcccaaa 1201 ttgcagaagg catggcattc attgaagagc ggaattatat tcatcgtgac cttcgggctg 1261 ccaacattct ggtgtctgac accctgagct gcaagattgc agactttggc ctagcacgcc 1321 tcattgagga caacgagtac acagccaggg agggggccaa gtttcccatt aagtggacag 1381 cgccagaagc cattaacTAC GGGACATTCA CCATCAAGTC AGATGTGTGG TCTTTTGGGA 1441 TCCTGCTGAC GGAAATTGTC ACCCACGGCC GCATCCCTTA CCCAGGGATG ACCAACCCGG 1501 AGGTGATTCA GAACCTGGAG CGAGGCTACC GCATGGTGCG CCCTGACAAC TGTCCAGAGG 1561 AGCTGTACCA ACTCATGAGG CTGTGCTGGA AGGAGCGCCC AGAGGACCGG CCCACCTTTG 1621 ACTACCTGCG CAGTGTGCTG GAGGACTTCT TCACGGCCAC AGAGGGCCAG TACCAGCCTC 1681 AGCCTTGAGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1741 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1801 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTTCTG GTCGTGTGGG CTGGGTCCAC 1861 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt