Construct: ORF TRCN0000491832
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014527.3_s317c1
- Derived from:
- ccsbBroadEn_14677
- DNA Barcode:
- AAATGGCACGCTTGATCTCGCTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITPKB (3707)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491832
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3707 | ITPKB | inositol-trisphosphate 3-ki... | XM_005273120.3 | 99.8% | 99.5% | 518G>A;1222T>G;1655C>A |
| 2 | human | 3707 | ITPKB | inositol-trisphosphate 3-ki... | XM_017001211.2 | 88% | 87.8% | (many diffs) |
| 3 | human | 3707 | ITPKB | inositol-trisphosphate 3-ki... | NM_002221.3 | 67.9% | 67.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1998
- ORF length:
- 1932
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgtgtactgc tatgcgctca atagcctggt gatcatgaat agcgccaacg 121 agatgaagag cggcggcggc ccggggccca gtggcagcga gacgcccccg cccccgagga 181 gggcagtgct gagccccggc agcgttttca gccccgggag aggcgcctct ttcctcttcc 241 ccccagccga gtcgctgtcc cccgaggagc cccggagccc cgggggctgg cggagcggcc 301 ggcgcaggct gaatagtagc agcggcagtg gcagcggcag cagcggcagt agcgtgagca 361 gcccaagttg ggctggtcgc ctgcgagggg accggcagca ggtggtggca gccggtaccc 421 tctccccgcc agggccggag gaggccaaga ggaagctgcg gatcttgcag cgcgagttgc 481 agaacgtgca ggtgaaccag aaagtgggca tgtttgaggc gcacatccag gcacagagct 541 ccgccattca agcgccccgc agcccgcgtt tgggcagggc tcactcgccc tccccgtgcc 601 ccttccgcag cagcagtcag ccccctggaa gggtcctggt tcagggcgcc cggagcgagg 661 aacggaggac aaagtcctgg ggggagcaat gtccagagac ttcaggaacc gactccggga 721 ggaaaggagg gcccagccta tgctcctcgc aggtgaagaa aggaatgcca cctcttcccg 781 gccgggctgc ccctacagga tcagaggctc agggtccatc cgcttttgta aggatggaga 841 agggtatccc tgccagtccc cgctgtggct cacccacagc tatggaaatt gacaaaaggg 901 gctctcctac cccgggaact cggagctgcc tagctccctc attggggctg ttcggagcta 961 gcttaacgat ggccacggaa gtggcagcga gagttacatc cactgggcca caccgtccac 1021 aggatcttgc cctcactgag ccgtctggga gagcccgtga gcttgaggac ctgcagcccc 1081 cagaggccct ggtggagagg caggggcagt ttctgggcag tgagacaagc ccagccccag 1141 aaaggggcgg gccccgcgat ggagaacccc ctgggaagat ggggaaagga tatctgccct 1201 gtggcatgcc gggctctggg gagcctgaag tgggcaaaag gccagaggag acgactgtga 1261 gcgtgcaaag cgcagagtcc tctgatgccc tgagctggtc caggctgccc agggccctgg 1321 cctccgtagg ccctgaggag gcccgaagtg gggcccccgt gggcgggggg cgttggcagc 1381 tctccgacag agtggaggga gggtccccaa cgctgggctt gcttgggggc agcccctcag 1441 cacagccggg gaccgggaat gtggaggcgg gaattccttc tggcagaatg ctggagcctt 1501 tgccctgttg ggacgctgcg aaagatctga aagaacctca gtgccctcct ggggacaggg 1561 tgggtgtgca gcctgggaac tccagggttt GGCAGGGCAC CATGGAGAAA GCCGGTTTGG 1621 CTTGGACGCG TGGCACAGGG GTGCAATCAG AGGGGACTTG GGAAAGCCAG CGGCAGGACA 1681 GTGATGCCCT CCCAAGTCCG GAGCTGCTAC CCCAAGATCA GGACAAGCCT TTCCTGAGGA 1741 AGGCCTGCAG CCCCAGCAAC ATACCTGCTG TCATCATTAC AGACATGGGC ACCCAGGAGG 1801 ATGGGGCCTT GGAGGAGACG CAGGGAAGCC CTCGGGGCAA CCTGCCCCTG AGGAAACTGT 1861 CCTCTTCCTC GGCCTCCTCC ACGGGCTTCT CCTCATCCTA CGAAGACTCA GAGGAGGACA 1921 TCTCCAGTGA CCCTGAGCGC ACCCTGGACC CCAACTCAGC CTTCCTGCAT ACCCTGGACC 1981 AGCAGAAACC TAGAGTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2041 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2101 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAAATGGC ACGCTTGATC 2161 TCGCTGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt