Construct: ORF TRCN0000491854

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019096.2_s317c1
DNA Barcode:
GAACAGTAACCGCTTATCCGTTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PFKFB3 (5209)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491854
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NM_004566.4 99.9% 99.8% 1560_1561insG
2 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NM_001314063.2 98.2% 97.1% (many diffs)
3 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... XM_017016329.2 96.2% 96.1% 440_441ins57;1503_1504insG
4 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NM_001145443.3 95.8% 94.6% (many diffs)
5 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NM_001282630.2 95.4% 92.5% (many diffs)
6 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NM_001323016.2 94.1% 91.9% (many diffs)
7 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NM_001363545.2 90.9% 89.2% (many diffs)
8 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... XM_005252464.2 88.7% 88.6% 1341_1342ins174;1386_1387insG
9 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... XM_017016326.2 87.5% 85.9% (many diffs)
10 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... XM_017016327.2 87.1% 84.5% (many diffs)
11 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... XM_017016328.2 84.7% 83% (many diffs)
12 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... XM_011519493.2 82% 81.9% 977_978ins105;1236_1237ins174;1281_1282insG
13 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NM_001323017.2 64.7% 64.6% 0_1ins549;1011_1012insG
14 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... XM_024448037.1 58.6% 57% (many diffs)
15 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NR_136554.2 28.9% (many diffs)
16 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_133232.3 88.4% 97.5% (many diffs)
17 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_001177754.1 87.3% 95.3% (many diffs)
18 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_001177757.1 85.9% 95% (many diffs)
19 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_001177758.1 84.7% 92.3% (many diffs)
20 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... XM_017315954.1 83.9% 92.8% (many diffs)
21 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_001177755.1 83.7% 92.3% (many diffs)
22 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_001177756.1 82.7% 90.3% (many diffs)
23 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_001177753.1 81.4% 90% (many diffs)
24 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... XM_006497367.1 78% 85.2% (many diffs)
25 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_001177752.1 77.9% 86.5% (many diffs)
26 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... XM_006497368.2 75.2% 83% (many diffs)
27 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... XM_006497369.2 75.2% 83% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1635
ORF length:
1563
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgccgttg gaactgacgc agagccgagt gcagaagatc tgggtgcccg
121tggaccacag gccctcgttg cccagatcct gtgggccaaa gctgaccaac tcccccaccg
181tcatcgtcat ggtgggcctc cccgcccggg gcaagaccta catctccaag aagctgactc
241gctacctcaa ctggattggc gtccccacaa aagtgttcaa cgtcggggag tatcgccggg
301aggctgtgaa gcagtacagc tcctacaact tcttccgccc cgacaatgag gaagccatga
361aagtccggaa gcaatgtgcc ttagctgcct tgagagatgt caaaagctac ctggcgaaag
421aagggggaca aattgcggtt ttcgatgcca ccaatactac tagagagagg agacacatga
481tccttcattt tgccaaagaa aatgacttta aggcgttttt catcgagtcg gtgtgcgacg
541accctacagt tgtggcctcc aatatcatgg aagttaaaat ctccagcccg gattacaaag
601actgcaactc ggcagaagcc atggacgact tcatgaagag gatcagttgc tatgaagcca
661gctaccagcc cctcgacccc gacaaatgcg acagggactt gtcgctgatc aaggtgattg
721acgtgggccg gaggttcctg gtgaaccggg tgcaggacca catccagagc cgcatcgtgt
781actacctgat gaacatccac gtgcagccgc gtaccatcta cctgtgccgg cacggcgaga
841acgagcacaa cctccagggc cgcatcgggg gcgactcagg cctgtccagc cggggcaaga
901agtttgccag tgctctgagc aagttcgtgg aggagcagaa cctgaaggac ctgcgcgtgt
961ggaccagcca gctgaagagc accatccaga cggccgaggc gctgcggctg ccctacgagc
1021agtggaaggc gctcaatgag atcgacgcgg gcgtctgtga ggagctgacc tacgaggaga
1081tcagggacac ctaccctgag gagtatgcgc tgcgggagca ggacaagtac tattaccgct
1141accccaccgg ggagtcctac caggacctgg tccagcgctt ggagccagtg atcatggagc
1201tggagcggca ggagaatgtg ctggtcatct gccaccaggc cgtccTGCGC TGCCTGCTTG
1261CCTACTTCCT GGATAAGAGT GCAGAGGAGA TGCCCTACCT GAAATGCCCT CTTCACACCG
1321TCCTGAAACT GACGCCTGTC GCTTATGGCT GCCGTGTGGA ATCCATCTAC CTGAACGTGG
1381AGTCCGTCTG CACACACCGG GAGAGGTCAG AGGATGCAAA GAAGGGACCT AACCCGCTCA
1441TGAGACGCAA TAGTGTCACC CCGCTAGCCA GCCCCGAACC CACCAAAAAG CCTCGCATCA
1501ACAGCTTTGA GGAGCATGTG GCCTCCACCT CGGCCGCCCT GCCCAGCTGC CTGCCCCCGG
1561AGGTGCCCAC GCAGCTGCCT GGACAAAACA TGAAAGGCTC CCGGAGCAGC GCTGACTCCT
1621CCAGGAAACA CGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1681ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1741CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAA CAGTAACCGC TTATCCGTTT
1801TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

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