Construct: ORF TRCN0000491875
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020118.2_s317c1
- DNA Barcode:
- AGCGCGGCTCCTGCCAGGGAAGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE1A (5136)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491875
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5136 | PDE1A | phosphodiesterase 1A | NM_001258314.1 | 76.4% | 76% | (many diffs) |
| 2 | human | 5136 | PDE1A | phosphodiesterase 1A | NM_001258313.1 | 73.8% | 73.4% | (many diffs) |
| 3 | human | 5136 | PDE1A | phosphodiesterase 1A | NM_001363871.2 | 72.7% | 72.7% | 1_372del;1528_1587delinsG |
| 4 | human | 5136 | PDE1A | phosphodiesterase 1A | NM_001003683.2 | 71.6% | 71.2% | (many diffs) |
| 5 | human | 5136 | PDE1A | phosphodiesterase 1A | XM_017004299.2 | 71.6% | 71.2% | (many diffs) |
| 6 | human | 5136 | PDE1A | phosphodiesterase 1A | XM_017004300.1 | 71.6% | 71.2% | (many diffs) |
| 7 | human | 5136 | PDE1A | phosphodiesterase 1A | XM_017004301.1 | 71.6% | 71.2% | (many diffs) |
| 8 | human | 5136 | PDE1A | phosphodiesterase 1A | XM_024452959.1 | 71.6% | 71.2% | (many diffs) |
| 9 | human | 5136 | PDE1A | phosphodiesterase 1A | NM_005019.5 | 70.6% | 70.6% | 1_420del;1576_1635delinsG |
| 10 | human | 5136 | PDE1A | phosphodiesterase 1A | XM_017004295.2 | 70.6% | 70.6% | 1_420del;1576_1635delinsG |
| 11 | human | 5136 | PDE1A | phosphodiesterase 1A | XM_017004296.2 | 70.6% | 70.6% | 1_420del;1576_1635delinsG |
| 12 | human | 5136 | PDE1A | phosphodiesterase 1A | XM_017004297.1 | 70.6% | 70.6% | 1_420del;1576_1635delinsG |
| 13 | human | 5136 | PDE1A | phosphodiesterase 1A | XM_017004298.1 | 70.6% | 70.6% | 1_420del;1576_1635delinsG |
| 14 | human | 5136 | PDE1A | phosphodiesterase 1A | NM_001258312.1 | 70.1% | 70.1% | 1_432del;1588_1647delinsG |
| 15 | human | 5136 | PDE1A | phosphodiesterase 1A | XM_011511326.2 | 69.6% | 69.3% | (many diffs) |
| 16 | human | 5136 | PDE1A | phosphodiesterase 1A | XM_011511324.3 | 69.3% | 69.2% | (many diffs) |
| 17 | human | 5136 | PDE1A | phosphodiesterase 1A | XM_011511323.2 | 67.7% | 67.5% | (many diffs) |
| 18 | human | 5136 | PDE1A | phosphodiesterase 1A | XM_011511325.3 | 67.4% | 67.1% | (many diffs) |
| 19 | human | 5136 | PDE1A | phosphodiesterase 1A | XM_017004294.2 | 65.5% | 65.3% | (many diffs) |
| 20 | human | 5136 | PDE1A | phosphodiesterase 1A | XR_002959304.1 | 61.5% | (many diffs) | |
| 21 | human | 5136 | PDE1A | phosphodiesterase 1A | XR_001738769.2 | 22.9% | 1_622del;1374_1519del;1924_5032delinsG | |
| 22 | mouse | 18573 | Pde1a | phosphodiesterase 1A, calmo... | NM_001009978.1 | 74.5% | 78.3% | (many diffs) |
| 23 | mouse | 18573 | Pde1a | phosphodiesterase 1A, calmo... | NM_001009979.1 | 73.3% | 76.9% | (many diffs) |
| 24 | mouse | 18573 | Pde1a | phosphodiesterase 1A, calmo... | XM_011239356.2 | 64.9% | 68.2% | (many diffs) |
| 25 | mouse | 18573 | Pde1a | phosphodiesterase 1A, calmo... | XM_006498923.3 | 64.7% | 68.2% | (many diffs) |
| 26 | mouse | 18573 | Pde1a | phosphodiesterase 1A, calmo... | NM_001159582.1 | 62.8% | 66.2% | (many diffs) |
| 27 | mouse | 18573 | Pde1a | phosphodiesterase 1A, calmo... | NM_016744.4 | 62.8% | 66.2% | (many diffs) |
| 28 | mouse | 18573 | Pde1a | phosphodiesterase 1A, calmo... | XM_006498919.3 | 62.8% | 66.2% | (many diffs) |
| 29 | mouse | 18573 | Pde1a | phosphodiesterase 1A, calmo... | XM_006498920.3 | 62.8% | 66.2% | (many diffs) |
| 30 | mouse | 18573 | Pde1a | phosphodiesterase 1A, calmo... | XM_006498921.3 | 62.8% | 66.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1227
- ORF length:
- 1158
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggttggtttg gcatatccag cagctgtcat cgtaacatta aaggatgttg 121 ataaatggtc tttcgatgta tttgccctaa atgaagcaag tggagagcat agtctgaagt 181 ttatgattta tgaactgttt accagatatg atcttatcaa ccgtttcaag attcctgttt 241 cttgcctaat cacctttgca gaagctttag aagttggtta cagcaagtac aaaaatccat 301 atcacaattt gattcatgca gctgatgtca ctcaaactgt gcattacata atgcttcata 361 caggtatcat gcactggctc actgaactgg aaattttagc aatggtcttt gctgctgcca 421 ttcatgatta tgagcataca gggacaacaa acaactttca cattcagaca aggtcagatg 481 ttgccatttt gtataatgat cgctctgtcc ttgagaatca ccacgtgagt gcagcttatc 541 gacttatgca agaagaagaa atgaatatct tgataaattt atccaaagat gactggaggg 601 atcttcggaa cctagtgatt gaaatggttt tatctacaga catgtcaggt cacttccagc 661 aaattaaaaa tataagaaac agtttgcagc agcctgaagg gattgacaga gccaaaacca 721 tgtccctgat tctccacgca gcagacatca gccacccagc caaatcctgg aagctgcatt 781 atcggtggac catggcccTA ATGGAGGAGT TTTTCCTGCA GGGAGATAAA GAAGCTGAAT 841 TAGGGCTTCC ATTTTCCCCA CTTTGTGATC GGAAGTCAAC CATGGTGGCC CAGTCACAAA 901 TAGGTTTCAT CGATTTCATA GTAGAGCCAA CATTTTCTCT TCTGACAGAC TCAACAGAGA 961 AAATTGTTAT TCCTCTTATA GAGGAAGCCT CAAAAGCCGA AACTTCTTCC TATGTGGCAA 1021 GCAGCTCAAC CACCATTGTG GGGTTACACA TTGCTGATGC ACTAAGACGA TCAAATACAA 1081 AAGGCTCCAT GAGTGATGGG TCCTATTCCC CAGACTACTC CCTTGCAGCA GTGGACCTGA 1141 AGAGTTTCAA GAACAACCTG GTGGACATCA TTCAGCAGAA CAAAGAGAGG TGGAAAGAGT 1201 TAGCTGCACA AGGTGAATCT GATGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1261 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1321 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GCGCGGCTCC 1381 TGCCAGGGAA GCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1441 att