Transcript: Human NM_001258313.1

Homo sapiens phosphodiesterase 1A (PDE1A), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-09-25
Taxon:
Homo sapiens (human)
Gene:
PDE1A (5136)
Length:
2338
CDS:
419..1978

Additional Resources:

NCBI RefSeq record:
NM_001258313.1
NBCI Gene record:
PDE1A (5136)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001258313.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000048752 CGTCGACTTAAAGAAGAATAT pLKO.1 517 CDS 100% 13.200 18.480 N PDE1A n/a
2 TRCN0000114999 GCTGAATTAGGGCTTCCATTT pLKO.1 1556 CDS 100% 10.800 15.120 N Pde1a n/a
3 TRCN0000048749 CCATGAGTGATGGGTCCTATT pLKO.1 1809 CDS 100% 10.800 8.640 N PDE1A n/a
4 TRCN0000412392 GATGCACTAAGACGATCAAAT pLKO_005 1778 CDS 100% 13.200 9.240 N PDE1A n/a
5 TRCN0000428468 TAAATGGTCTTTCGATGTATT pLKO_005 844 CDS 100% 13.200 9.240 N PDE1A n/a
6 TRCN0000048748 CCACATAAATAACAGCTGTAA pLKO.1 2125 3UTR 100% 4.950 3.465 N PDE1A n/a
7 TRCN0000048751 CGACTTATGCAAGAAGAAGAA pLKO.1 1262 CDS 100% 4.950 3.465 N PDE1A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001258313.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491875 AGCGCGGCTCCTGCCAGGGAAGCG pLX_317 37% 73.8% 73.4% V5 (many diffs) n/a
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