Construct: ORF TRCN0000491887
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018391.1_s317c1
- Derived from:
- ccsbBroadEn_10688
- DNA Barcode:
- TGGTATTGATTCTCGAAGGTGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATM (472)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491887
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 472 | ATM | ATM serine/threonine kinase | NM_001351835.1 | 100% | 100% | |
| 2 | human | 472 | ATM | ATM serine/threonine kinase | NM_001351836.1 | 100% | 100% | |
| 3 | human | 472 | ATM | ATM serine/threonine kinase | XM_017017792.2 | 6.2% | 6.1% | 332_333insTA;335_5325del |
| 4 | human | 472 | ATM | ATM serine/threonine kinase | XM_017017791.1 | 5.1% | 5% | 332_333insTA;335_6474del |
| 5 | human | 472 | ATM | ATM serine/threonine kinase | XR_002957150.1 | 5% | 1_733del;1065_1066insTA;1068_6577del | |
| 6 | human | 472 | ATM | ATM serine/threonine kinase | XM_011542843.2 | 3.8% | 3.7% | 332_333insTA;335_8736del |
| 7 | human | 472 | ATM | ATM serine/threonine kinase | XM_011542842.3 | 3.7% | 3.6% | 332_344del;348_351delCTTC;354_9003del |
| 8 | human | 472 | ATM | ATM serine/threonine kinase | NM_000051.3 | 3.6% | 3.5% | 332_333insTA;335_9168del |
| 9 | human | 472 | ATM | ATM serine/threonine kinase | NM_001351834.2 | 3.6% | 3.5% | 332_333insTA;335_9168del |
| 10 | human | 472 | ATM | ATM serine/threonine kinase | XM_005271562.5 | 3.6% | 3.5% | 332_333insTA;335_9168del |
| 11 | human | 472 | ATM | ATM serine/threonine kinase | XM_006718843.4 | 3.6% | 3.5% | 332_333insTA;335_9168del |
| 12 | human | 472 | ATM | ATM serine/threonine kinase | XM_011542840.3 | 3.6% | 3.5% | 332_333insTA;335_9168del |
| 13 | human | 472 | ATM | ATM serine/threonine kinase | XM_017017789.2 | 3.6% | 3.5% | 332_333insTA;335_9168del |
| 14 | human | 472 | ATM | ATM serine/threonine kinase | XM_017017790.2 | 3.6% | 3.5% | 332_333insTA;335_9168del |
| 15 | mouse | 11920 | Atm | ataxia telangiectasia mutated | XM_011242386.1 | 4.4% | 4.4% | (many diffs) |
| 16 | mouse | 11920 | Atm | ataxia telangiectasia mutated | XR_870525.1 | 3.5% | (many diffs) | |
| 17 | mouse | 11920 | Atm | ataxia telangiectasia mutated | XM_006509956.3 | 3.1% | 3.1% | (many diffs) |
| 18 | mouse | 11920 | Atm | ataxia telangiectasia mutated | NM_007499.2 | 3.1% | 3.1% | (many diffs) |
| 19 | mouse | 11920 | Atm | ataxia telangiectasia mutated | XM_011242385.2 | 3.1% | 3.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 402
- ORF length:
- 336
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tctagtactt aatgaTCTGC TTATCTGCTG CCGTCAACTA GAACATGATA 121 GAGCTACAGA ACGAAAGAAA GAAGTTGAGA AATTTAAGCG CCTGATTCGA GATCCTGAAA 181 CAATTAAACA TCTAGATCGG CATTCAGATT CCAAACAAGG AAAATATTTG AATTGGGATG 241 CTGTTTTTAG ATTTTTACAG AAATATATTC AGAAAGAAAC AGAATGTCTG AGAATAGCAA 301 AACCAAATGT ATCAGCCTCA ACACAAGCCT CCAGGCAGAA AAAGATGCAG GAAATCAGTA 361 GTTTGGTCAA ATACTTCATC AAATGTGCAA ACAGAAGTAA GTGCCCAACT TTCTTGTACA 421 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 481 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 541 AGGACGATGG TATTGATTCT CGAAGGTGCC GACGCGTTAA GTCgacaatc aacctctgga 601 ttacaaaatt tgtgaaagat t