Construct: ORF TRCN0000491921
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012065.1_s317c1
- Derived from:
- ccsbBroadEn_07292
- DNA Barcode:
- CGAGAAGCCCCTTATGACGTCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNFSF13 (8741)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491921
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8741 | TNFSF13 | TNF superfamily member 13 | NM_172088.2 | 99.8% | 99.5% | 287A>G |
| 2 | human | 8741 | TNFSF13 | TNF superfamily member 13 | NM_003808.3 | 98.4% | 98% | 287A>G;738_739delGTinsAC;742_750delGTGAAACTG |
| 3 | human | 8741 | TNFSF13 | TNF superfamily member 13 | NM_172087.2 | 92% | 91.2% | (many diffs) |
| 4 | human | 8741 | TNFSF13 | TNF superfamily member 13 | NM_001198622.1 | 87.7% | 87.6% | 256_257ins81;657_658delGTinsAC;661_669delGTGAAACTG |
| 5 | human | 8741 | TNFSF13 | TNF superfamily member 13 | NM_001198623.1 | 87.3% | 87.2% | 253_254ins84;654_655delGTinsAC;658_666delGTGAAACTG |
| 6 | human | 8741 | TNFSF13 | TNF superfamily member 13 | NM_001198624.1 | 80.5% | 80.4% | (many diffs) |
| 7 | human | 407977 | TNFSF12-TNFSF13 | TNFSF12-TNFSF13 readthrough | NM_172089.4 | 65.7% | 55.9% | (many diffs) |
| 8 | human | 8741 | TNFSF13 | TNF superfamily member 13 | NR_073490.2 | 26.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 807
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc agcctcatct cctttcttgc tagcccccaa agggcctcca ggcaacatgg 121 ggggcccagt cagagagccg gcactctcag ttgccctctg gttgagttgg ggggcagctc 181 tgggggccgt ggcttgtgcc atggctctgc tgacccaaca aacagagctg cagagcctca 241 ggagagaggt gagccggctg caggggacag gaggcccctc ccagaatggg gaagggtatc 301 cctggcagag tctcccggag cagagttccg atgccctgga agcctgggag agtggggaga 361 gatcccggaa aaggagagca gtgctcaccc aaaaacagaa gaagcagcac tctgtcctgc 421 acctggttcc cattaacgcc acctccaagg atgacTCCGA TGTGACAGAG GTGATGTGGC 481 AACCAGCTCT TAGGCGTGGG AGAGGCCTAC AGGCCCAAGG ATATGGTGTC CGAATCCAGG 541 ATGCTGGAGT TTATCTGCTG TATAGCCAGG TCCTGTTTCA AGACGTGACT TTCACCATGG 601 GTCAGGTGGT GTCTCGAGAA GGCCAAGGAA GGCAGGAGAC TCTATTCCGA TGTATAAGAA 661 GTATGCCCTC CCACCCGGAC CGGGCCTACA ACAGCTGCTA TAGCGCAGGT GTCTTCCATT 721 TACACCAAGG GGATATTCTG AGTGTCATAA TTCCCCGGGC AAGGGCGAAA CTTAACCTCT 781 CTCCACATGG AACCTTCCTG GGACTTTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 841 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 901 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGAGAAGC 961 CCCTTATGAC GTCTTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1021 aagatt