Construct: ORF TRCN0000491921

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012065.1_s317c1
Derived from:
ccsbBroadEn_07292
DNA Barcode:
CGAGAAGCCCCTTATGACGTCTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TNFSF13 (8741)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491921
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8741 TNFSF13 TNF superfamily member 13 NM_172088.2 99.8% 99.5% 287A>G
2 human 8741 TNFSF13 TNF superfamily member 13 NM_003808.3 98.4% 98% 287A>G;738_739delGTinsAC;742_750delGTGAAACTG
3 human 8741 TNFSF13 TNF superfamily member 13 NM_172087.2 92% 91.2% (many diffs)
4 human 8741 TNFSF13 TNF superfamily member 13 NM_001198622.1 87.7% 87.6% 256_257ins81;657_658delGTinsAC;661_669delGTGAAACTG
5 human 8741 TNFSF13 TNF superfamily member 13 NM_001198623.1 87.3% 87.2% 253_254ins84;654_655delGTinsAC;658_666delGTGAAACTG
6 human 8741 TNFSF13 TNF superfamily member 13 NM_001198624.1 80.5% 80.4% (many diffs)
7 human 407977 TNFSF12-TNFSF13 TNFSF12-TNFSF13 readthrough NM_172089.4 65.7% 55.9% (many diffs)
8 human 8741 TNFSF13 TNF superfamily member 13 NR_073490.2 26.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
807
ORF length:
741
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc agcctcatct cctttcttgc tagcccccaa agggcctcca ggcaacatgg
121ggggcccagt cagagagccg gcactctcag ttgccctctg gttgagttgg ggggcagctc
181tgggggccgt ggcttgtgcc atggctctgc tgacccaaca aacagagctg cagagcctca
241ggagagaggt gagccggctg caggggacag gaggcccctc ccagaatggg gaagggtatc
301cctggcagag tctcccggag cagagttccg atgccctgga agcctgggag agtggggaga
361gatcccggaa aaggagagca gtgctcaccc aaaaacagaa gaagcagcac tctgtcctgc
421acctggttcc cattaacgcc acctccaagg atgacTCCGA TGTGACAGAG GTGATGTGGC
481AACCAGCTCT TAGGCGTGGG AGAGGCCTAC AGGCCCAAGG ATATGGTGTC CGAATCCAGG
541ATGCTGGAGT TTATCTGCTG TATAGCCAGG TCCTGTTTCA AGACGTGACT TTCACCATGG
601GTCAGGTGGT GTCTCGAGAA GGCCAAGGAA GGCAGGAGAC TCTATTCCGA TGTATAAGAA
661GTATGCCCTC CCACCCGGAC CGGGCCTACA ACAGCTGCTA TAGCGCAGGT GTCTTCCATT
721TACACCAAGG GGATATTCTG AGTGTCATAA TTCCCCGGGC AAGGGCGAAA CTTAACCTCT
781CTCCACATGG AACCTTCCTG GGACTTTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG
841GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
901AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGAGAAGC
961CCCTTATGAC GTCTTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1021aagatt

Download FASTA (ORF) (Full)