Construct: ORF TRCN0000491923
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006033.1_s317c1
- Derived from:
- ccsbBroadEn_04964
- DNA Barcode:
- TTACTCCAGGGGGTACAGAGTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYT9 (143425)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491923
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 143425 | SYT9 | synaptotagmin 9 | NM_175733.4 | 99.9% | 100% | 351T>C |
| 2 | human | 143425 | SYT9 | synaptotagmin 9 | XM_011519904.2 | 99.1% | 99.3% | 351T>C;1467_1468insAAAC;1469_1476delCTCATGGGinsA |
| 3 | human | 143425 | SYT9 | synaptotagmin 9 | XM_011519901.2 | 86.9% | 86.7% | (many diffs) |
| 4 | human | 143425 | SYT9 | synaptotagmin 9 | XM_011519900.2 | 86.4% | 86.2% | (many diffs) |
| 5 | human | 143425 | SYT9 | synaptotagmin 9 | XM_011519902.2 | 80.1% | 77.7% | (many diffs) |
| 6 | human | 143425 | SYT9 | synaptotagmin 9 | XM_011519906.2 | 71.2% | 70.8% | (many diffs) |
| 7 | human | 143425 | SYT9 | synaptotagmin 9 | XM_011519907.2 | 70.9% | 70.8% | 351T>C;1046G>A;1047_1048ins426 |
| 8 | human | 143425 | SYT9 | synaptotagmin 9 | XR_001747772.1 | 29.7% | (many diffs) | |
| 9 | human | 143425 | SYT9 | synaptotagmin 9 | XR_001747773.1 | 28.8% | (many diffs) | |
| 10 | mouse | 60510 | Syt9 | synaptotagmin IX | NM_021889.4 | 90.8% | 97.5% | (many diffs) |
| 11 | mouse | 60510 | Syt9 | synaptotagmin IX | XM_006508099.3 | 69.1% | 65.6% | (many diffs) |
| 12 | mouse | 60510 | Syt9 | synaptotagmin IX | XR_378262.4 | 31.3% | (many diffs) | |
| 13 | mouse | 60510 | Syt9 | synaptotagmin IX | XR_003946532.1 | 26.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1539
- ORF length:
- 1473
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cggggccagg gacgcgctct gtcaccaggc gctgcagctg ctggccgagc 121 tctgtgcccg tggggccctg gagcacgaca gctgccagga tttcatttac cacctgcggg 181 accgtgccag accccggctc cgcgacccag atatctcagt gagcctgctg acccttgtgg 241 tcactgcctg tggtctcgct ctctttggcg tgtctctctt cgtatcttgg aaactctgct 301 gggttccgtg gcgagaacga ggcctgccct ctggtagcaa agacaacaac caggagcccc 361 ttaactacat ggacacagag accaatgagc aggagaacag tgaggacttc ctagaccctc 421 ccacgccctg ccctgactcc tccatgaaga tcagccacac ctcccctgac attcccctct 481 ccacccagac ggggatccag gagaactgtg cccatggcgt ccgcgtgcag cgccaagtca 541 cagagccaac ctcgtcggcc cggcataatt caatccgaag acaactcaac ttgtcaaacc 601 cggacttcaa tatccagcag cttcaaaaac aggaacagtt gactggaatt ggtagaatta 661 aaccagagtt atataagcag aggtcattgg ataatgatga cgggagacgg agtaacagca 721 aggcttgtgg gaaactgaac ttcattttaa aatatgactg tgacttagag cagctcatag 781 tgaagattca caaagctgtc aatttgcccg ccaaggactt ttctgggact tcagatcctt 841 atgtcaagat ctatttgctt cctgatcgga aaacaaaaca ccagactaaa gttcacagaa 901 agaccctgaa ccctgtgttt gatgaagtgt ttttatttcc ggttccctac aatgaccttg 961 aagcacggaa gcttcacttc tctgtgtacg actttgacag gttctctcgt catgacttaa 1021 tcggccaagt ggtggtggat cacttcctag acttggctga tttccccagg gagtgcatcc 1081 tttggaagga tatcgaatat gtcaccaatg acaacgtgga tctgggagag ctgatgtttt 1141 ccctgtgcta TCTTCCAACG GCTGGCAGGC TGACCATTAC CATTATAAAA GCAAGGAATT 1201 TAAAGGCAAT GGACATAACA GGAGCATCAG ATCCCTATGT GAAAGTCTCG CTGATGTGTG 1261 ATGGCAGACG ACTGAAGAAG AGGAAAACAT CCACCAAGAG GAACACCTTG AATCCTGTTT 1321 ACAACGAAGC CATAGTCTTT GATGTCCCTC CCGAGAACAT TGACCAAATC CACTTGTCCA 1381 TAGCAGTCAT GGACTATGAC CGTGTAGGTC ACAATGAGAT CATCGGCGTG TGTCAAGTAG 1441 GCAACGAGGC TGAGAGGCTG GGCAGAGACC ACTGGAGTGA AATGTTGTCA TATCCTCGGA 1501 AGCCCATTGC ACACTGGCAT TCCCTGGTGG AGAAACGATG CCCAACTTTC TTGTACAAAG 1561 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1621 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1681 ACGATTACTC CAGGGGGTAC AGAGTTTTAC GCGTTAAGTC gacaatcaac ctctggatta 1741 caaaatttgt gaaagatt