Construct: ORF TRCN0000491933
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007891.1_s317c1
- Derived from:
- ccsbBroadEn_01409
- DNA Barcode:
- GGATTTGAACTAAGAGAGCCATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF6 (6049)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491933
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6049 | RNF6 | ring finger protein 6 | NM_005977.4 | 100% | 100% | |
| 2 | human | 6049 | RNF6 | ring finger protein 6 | NM_183043.2 | 100% | 100% | |
| 3 | human | 6049 | RNF6 | ring finger protein 6 | NM_183044.2 | 100% | 100% | |
| 4 | human | 6049 | RNF6 | ring finger protein 6 | XM_005266485.3 | 100% | 100% | |
| 5 | human | 6049 | RNF6 | ring finger protein 6 | XM_005266486.2 | 100% | 100% | |
| 6 | human | 6049 | RNF6 | ring finger protein 6 | XM_011535177.3 | 100% | 100% | |
| 7 | human | 6049 | RNF6 | ring finger protein 6 | XM_024449390.1 | 100% | 100% | |
| 8 | human | 6049 | RNF6 | ring finger protein 6 | XM_017020685.2 | 25.6% | 21% | 408_409ins704;528_529ins823 |
| 9 | human | 6049 | RNF6 | ring finger protein 6 | XM_017020686.1 | 25.6% | 21% | 408_409ins704;528_529ins823 |
| 10 | human | 6049 | RNF6 | ring finger protein 6 | XM_017020687.1 | 25.6% | 21% | 408_409ins704;528_529ins823 |
| 11 | human | 6049 | RNF6 | ring finger protein 6 | XM_024449391.1 | 25.6% | 21% | 408_409ins704;528_529ins823 |
| 12 | human | 6049 | RNF6 | ring finger protein 6 | XM_024449392.1 | 25.6% | 21% | 408_409ins704;528_529ins823 |
| 13 | human | 6049 | RNF6 | ring finger protein 6 | NM_183045.1 | 20.2% | 20% | (many diffs) |
| 14 | human | 6049 | RNF6 | ring finger protein 6 | XM_011535178.2 | 20.2% | 20% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2121
- ORF length:
- 2055
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tcagtctaga tcgagatcag atggtggcag tgaagaaacc ttacctcaag 121 accataatca tcatgaaaat gagagaagat ggcagcaaga gcgtctccac agagaagagg 181 cctattatca gtttattaat gaactcaatg atgaagatta tcggcttatg agagaccata 241 atcttttagg cacccctgga gaaataacat cagaagaact gcaacagcgg ttagatggcg 301 tcaaggaaca actagcatct cagcctgact tgagagatgg aacgaattac agagactcag 361 aagtccctag agaaagttca catgaagatt ctcttctaga atggttgaac acctttcggc 421 gcacaggaaa tgcaactcga agtggacaaa atgggaacca aacttggaga gctgtgagtc 481 gaacaaaccc gaacaatgga gagtttcggt ttagtttgga aatccacgta aatcatgaaa 541 atagaggatt tgaaattcat ggagaagatt atacagacat tccactttca gatagtaaca 601 gagatcatac tgcaaatagg caacaaaggt caactagtcc tgtggctagg cgaacaagaa 661 gccaaacctc agtgaatttc aatggtagta gttccaacat tccaaggact aggcttgctt 721 caagggggca aaatccagct gaaggatctt tctcaacatt gggaaggtta agaaatggaa 781 ttgggggagc agctggcatt cctcgagcta acgcttcacg cactaatttc agtagtcaca 841 caaaccaatc aggtggtagt gaactcaggc aaagggaggg gcaacggttt ggagcagcac 901 atgtttggga aaatggggct agaagtaatg ttacagtgag gaatacaaac caaagattag 961 agccaataag attacgatct acttccaata gtcgaagccg ttcaccaatt cagagacaga 1021 gtggcactgt ttatcataat tcccaaaggg aaagtagacc agtacagcaa accactagaa 1081 gatctgttag gaggagaggt agaactcgag tctttttaga gcaagataga gaacgagaac 1141 gcagaggtac tgcatatacc ccattctcta attcaaggct tgtgtcaaga ataacagtag 1201 aagaaggaga agaatccagc agatcctcaa ctgctgtacg acgacatcca acaatcacac 1261 tggaccttca agtgagaagg atccgtcctg gagaaaatag agatcgggat agtattgcaa 1321 atagaactcg atccagagta gggctagcag aaaatacagt cactattgaa agcaatagtg 1381 ggggctttcg ccgaaccatt tctcgtttag agcggtcagg tattcgaacc tatgttagta 1441 ccataacagt tcctcttcgt aggatttctg agaatgagct tgttgagcca tcatcagtgg 1501 ctcttcggtc aattttaagg cagatcatga ctgggtttgg agaactgagt tctctaatgg 1561 aggccgattc tgagtcagaa cttcaaagaa atggccagca tttaccagac atgcactcag 1621 aactgagtaa cttaggtaca gataacaaca ggagccagca cagggaaggt tcctctcaag 1681 acaggcaggC CCAAGGAGAC AGCACTGAAA TGCATGGTGA AAACGAGACC ACCCAGCCTC 1741 ATACTCGAAA CAGTGACAGT AGGGGTGGCA GGCAGTTGCG AAATCCAAAC AATTTAGTTG 1801 AAACTGGAAC ACTACCCATT CTTCGCCTTG CTCACTTTTT TTTACTAAAT GAAAGTGATG 1861 ATGATGATCG AATACGTGGT TTAACCAAAG AGCAGATTGA CAATCTTTCC ACCAGGCACT 1921 ATGAGCATAA CAGTATTGAT AGTGAACTAG GTAAAATCTG TAGTGTTTGT ATTAGTGACT 1981 ATGTAACTGG AAACAAGCTC AGGCAATTAC CTTGCATGCA TGAATTTCAC ATTCATTGTA 2041 TTGACCGATG GCTCTCAGAG AATTGCACTT GTCCGATCTG TCGGCAGCCT GTTTTAGGGT 2101 CTAACATAGC AAACAATGGG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2161 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2221 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGAT TTGAACTAAG 2281 AGAGCCATTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt