Construct: ORF TRCN0000491942
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020426.2_s317c1
- DNA Barcode:
- CCTATCCGGCCGAGTTACGAGTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PIK3C3 (5289)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491942
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | NM_002647.4 | 99.9% | 100% | 1413G>C;1416A>G |
| 2 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | NM_001308020.2 | 92.8% | 92.8% | 68_69ins189;1224G>C;1227A>G |
| 3 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | XM_011526031.3 | 78.9% | 79% | 0_1ins558;855G>C;858A>G |
| 4 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | XR_002958176.1 | 41.5% | (many diffs) | |
| 5 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | XR_935226.1 | 40.1% | (many diffs) | |
| 6 | human | 5289 | PIK3C3 | phosphatidylinositol 3-kina... | XR_001753204.2 | 39.1% | (many diffs) | |
| 7 | mouse | 225326 | Pik3c3 | phosphatidylinositol 3-kina... | NM_181414.5 | 87.8% | 97.9% | (many diffs) |
| 8 | mouse | 225326 | Pik3c3 | phosphatidylinositol 3-kina... | XR_385985.2 | 58.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 2745
- ORF length:
- 2661
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatggggg aagcagagaa gtttcactac atctatagtt 121 gtgacctgga tatcaacgtc cagcttaaga taggaagctt ggaagggaag agagaacaaa 181 agagttataa agctgtcctg gaagacccaa tgttgaagtt ctcaggacta tatcaagaga 241 catgctctga tctttatgtt acttgtcaag tttttgcaga agggaagcct ttggccttgc 301 cagtgagaac atcctacaaa gcatttagta caagatggaa ctggaatgaa tggctgaaac 361 taccagtaaa ataccctgac ctgcccagga atgcccaagt ggccctcacc atatgggatg 421 tgtatggtcc cggaaaagca gtgcctgtag gaggaacaac ggtttcgctc tttggaaaat 481 acggcatgtt tcgccaaggg atgcatgact tgaaagtctg gcctaatgta gaagcagatg 541 gatcagaacc cacaaaaact cctggcagaa caagtagcac tctctcagaa gatcagatga 601 gccgtcttgc caagctcacc aaagctcatc gacaaggaca catggtgaaa gtagattggc 661 tggatagatt gacatttaga gaaatagaaa tgataaatga gagtgaaaaa cgaagttcta 721 atttcatgta cctgatggtt gaatttcgat gtgtcaagtg tgatgataag gaatatggta 781 ttgtttatta tgaaaaggac ggtgatgaat catctccaat tttaacaagt tttgaattag 841 tgaaagttcc tgacccccag atgtctatgg agaatttagt tgagagcaaa caccacaagc 901 ttgcccggag tttaagaagt ggaccttctg accacgatct gaaacccaat gctgccacga 961 gagatcagtt aaatattatt gtgagttatc caccaaccaa gcaacttaca tatgaagaac 1021 aagatcttgt ttggaagttt agatattatc ttacgaatca agaaaaagcc ttgacaaaat 1081 tcttgaaatg tgttaattgg gatctacctc aagaggccaa acaggccttg gaacttctgg 1141 gaaaatggaa gccgatggat gtagaggact ccttggagct gttatcctct cattacacca 1201 acccaactgt gaggcgttat gctgttgccc ggttgcgaca ggccgatgat gaggatttgt 1261 tgatgtacct attacaattg gtccaggctc tcaaatatga aaattttgat gatataaaga 1321 atggattgga acctaccaag aaggatagtc agagttcagt gtcagaaaat gtgtcaaatt 1381 ctggaataaa ttctgcagaa atagatagct cccaaattat aaccagcccc cttccttcag 1441 tctcttcacc tcctcctgca tcaaaaacaa aagaagttcc agatggcgaa aatctcgagc 1501 aagatctctg taccttcttg atatcgagag cctgcaaaaa ctcaacactg gctaattatt 1561 tatactggta tgtgatagtg gaatgtgaag atcaagatac tcagcagaga gatccaaaga 1621 cccatgagat gtacttgaac gtaatgagaa gattcagcca agcattgttg aagggtgata 1681 agtctgtcag agttatgcgt tctttgctgg ctgcacaaca gacatttgta gatcggttgg 1741 tgcatctaat gaaggcagta caacgcgaaa gtggaaatcg taagaaaaag aatgagagac 1801 tacaggcatt gcttggagat aatgaaaaga tgaatttgtc agatgtggaa cttatcccgt 1861 tgcctttaga accccaagtg aaaattagag gaataattcc ggaaacagct acactgttta 1921 aaagtgccct tatgcctgca cagttgtttt ttaagacgga agatggaggc aaatatccag 1981 ttatatttaa gcatggagat gatttacgtc aagatcaact tattcttcaa atcatttcac 2041 tcatggacaa gctgttacgg aaagaaaatc tggacttgaa attgacacct tataaggtgt 2101 tagccaccag tacaaaacat ggcttcatgc agtttatcca gtcagttcct gtggctgaag 2161 ttcttgatac agagggaagc attcagaact tttttagaaa atatgcacca agtgagaatg 2221 ggccaaatgg gattagtgct gaggtcatgg acacttacgt taaaagctgt gctggatatt 2281 gcgtgatcac ctatatactt ggagttggag acaggcacct ggataacctt ttgctaacaa 2341 aaacaggcaa actcttccac atagactttg gatatatttt GGGTCGGGAT CCAAAGCCTC 2401 TTCCTCCACC AATGAAGCTG AATAAAGAAA TGGTAGAAGG AATGGGGGGC ACACAGAGTG 2461 AGCAGTACCA AGAGTTCCGT AAACAGTGTT ACACGGCTTT CCTCCACCTG CGAAGGTATT 2521 CTAATCTGAT TTTGAACTTG TTTTCCTTGA TGGTTGATGC AAACATTCCA GATATTGCAC 2581 TTGAACCAGA TAAAACTGTG AAAAAGGTTC AGGATAAATT CCGCTTAGAC CTGTCGGATG 2641 AAGAGGCTGT GCATTACATG CAGAGTCTGA TTGATGAGAG TGTCCATGCT CTTTTTGCTG 2701 CAGTGGTGGA ACAGATTCAC AAGTTTGCCC AGTACTGGAG AAAATGATCT AGAAAGGGTG 2761 GGCGCGCCGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2821 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2881 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCTATC CGGCCGAGTT ACGAGTCCAC 2941 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt