Construct: ORF TRCN0000491944
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010323.1_s317c1
- Derived from:
- ccsbBroadEn_02933
- DNA Barcode:
- CCAGCCGGTCTCAAAACAGGCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAP1 (26228)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491944
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26228 | STAP1 | signal transducing adaptor ... | NM_001317769.2 | 100% | 100% | |
| 2 | human | 26228 | STAP1 | signal transducing adaptor ... | NM_012108.4 | 100% | 100% | |
| 3 | human | 26228 | STAP1 | signal transducing adaptor ... | XM_017008018.2 | 72.7% | 60.3% | (many diffs) |
| 4 | mouse | 56792 | Stap1 | signal transducing adaptor ... | NM_019992.4 | 84.2% | 83.1% | (many diffs) |
| 5 | mouse | 56792 | Stap1 | signal transducing adaptor ... | NM_001310639.1 | 73.8% | 72.7% | (many diffs) |
| 6 | mouse | 56792 | Stap1 | signal transducing adaptor ... | XM_006535161.3 | 35.6% | 36.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 951
- ORF length:
- 885
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggctaagaag cccccaaaac cagcccctcg caggatcttc caggaaaggt 121 taaagattac tgctctacct ttgtactttg aaggtttttt attaatcaag cggtcaggat 181 accgggagta tgagcattac tggacagagt tgagaggaac tactcttttc ttttataccg 241 acaaaaagag tataatatat gttgacaaat tagacatagt agacctcaca tgccttactg 301 agcagaattc aactgaaaag aactgtgcga aattcaccct tgttttgccg aaagaggaag 361 tacaactgaa gacagagaac acagaaagtg gggaagaatg gagaggcttc attcttacag 421 taacagagct gtcagttccc caaaacgtgt cactcctacc tgggcaagta attaaactgc 481 atgaagtcct agagagagaa aagaaaagga ggattgagac agagcagagt acgtccgtgg 541 aaaaagagaa ggaaccaact gaagattatg tggatgtact gaaccctatg ccagcatgtt 601 tttatacagt gtcccggaaa gaggcaacTG AGATGCTCCA GAAGAACCCT TCTTTGGGAA 661 ATATGATCCT GAGGCCTGGT AGTGACAGTA GAAACTACTC CATCACTATT CGGCAGGAGA 721 TAGACATTCC AAGAATCAAG CACTACAAAG TGATGAGCGT AGGACAAAAC TACACTATTG 781 AACTGGAAAA ACCTGTAACA CTCCCAAACC TTTTCAGTGT CATTGATTAT TTTGTGAAGG 841 AGACTCGAGG AAATTTAAGA CCATTTATAT GTTCAACTGA TGAAAACACT GGTCAAGAAC 901 CCAGTATGGA AGGGAGAAGT GAAAAGTTGA AGAAAAATCC ACACATTGCA TGCCCAACTT 961 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1021 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1081 CTTGTGGAAA GGACGACCAG CCGGTCTCAA AACAGGCGGT ACGCGTTAAG TCgacaatca 1141 acctctggat tacaaaattt gtgaaagatt