Construct: ORF TRCN0000492015

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018985.1_s317c1
Derived from:
ccsbBroadEn_15077
DNA Barcode:
CTTCTATTCAAAGCCTATCACCAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PRKAG3 (53632)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492015
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 53632 PRKAG3 protein kinase AMP-activate... NM_017431.3 99.2% 48.6% (many diffs)
2 human 53632 PRKAG3 protein kinase AMP-activate... XM_017004343.2 99.2% 48.6% (many diffs)
3 mouse 241113 Prkag3 protein kinase, AMP-activat... NM_153744.3 84.9% 35.3% (many diffs)
4 mouse 241113 Prkag3 protein kinase, AMP-activat... XM_006495988.3 84.9% 35.3% (many diffs)
5 mouse 241113 Prkag3 protein kinase, AMP-activat... XM_006495989.3 84.9% 35.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
801
ORF length:
732
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagcccggg ctggagcacg cactgcgcag gaccccttcc tggagcagcc
121ttgggggttc tgagcatcaa gagatgagct tcctagagca agaaaacagc agctcatggc
181catcaccagc tgtgaccagc agctcagaaa gaatccgtgg gaaacggagg gccaaagcct
241tgagatggac aaggcagaag tcggtggagg aaggggagcc accaggtcag ggggaaggtc
301cccggtccag gccagctgct gagtccaccg ggctggaggc cacattcccc aagaccacac
361ccttggctca agctgatcct gccggggtgg gcactccacc aacagggtgg gactgcctcc
421cctctgactg tacagcctca gctgcaggct ccagcacaga tgatgtggag ctggccacgg
481agttcccagc cacagaggcc tgggagtgtg agctagaagg cctgctggaa gagaggcctg
541ccctgtgcct gtccccgcag gccccatttc ccaagctggg ctgggatgac gaactgcgga
601aacccggcgc ccagatctac atgcgcttca tgcaggagca cacctgctac gatgccatgg
661caactagctc caagctagtc atcttcgaca ccatgctgga gatcaagaag gccttctttg
721ctctggtggc caacggggtg cgggcagccc ctctatggga cagcaagaag cagagctttt
781gtggggatgc tgaccatcac tgactttttc ctggtgcggc atcgttattc caggtccccc
841cttgtcccag atctatgaga ttgaacaaca taagattgag acctggaggg agatctacct
901gcaaggctgc ttcaagcttc tggtctccat ctctcctaat gatagcctgt ttgaagctgt
961ctacaccctc atcaagaacc ggatccatcg cctgcctgtt cttgacccgg tgtcaggcaa
1021cgtactccac atcctcacac acaaacgcct gctcaagttc ctgcacatct ttggttccct
1081gctgccccgg ccctccttcc tctaccgcac tatccaagat ttgggcatcg gcacattccg
1141agacttggct gtggtgctGG AGACAGCACC CATCCTGACT GCACTGGACA TCTTTGTGGA
1201CCGGCGTGTG TCTGCACTGC CTGTGGTCAA CGAATGTGGT CAGGTCGTGG GCCTCTATTC
1261CCGCTTTGAT GTGATTCACC TGGCTGCCCA GCAAACCTAC AACCACCTGG ACATGAGTGT
1321GGGAGAAGCC CTGAGGCAGA GGACACTATG TCTGGAGGGA GTCCTTTCCT GCCAGCCCCA
1381CGAGAGCTTG GGGGAAGTGA TCGACAGGAT TGCTCGGGAG CAGGTACACA GGCTGGTGCT
1441AGTGGACGAG ACCCAGCATC TCTTGGGCGT GGTCTCCCTC TCCGACATCC TTCAGGCACT
1501GGTGCTCAGC CCTGCTGGCA TCGATGCCCT CGGGGCCTTG CCAACTTTCT TGTACAAAGT
1561GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG
1621AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA
1681CGACTTCTAT TCAAAGCCTA TCACCACACG CGTTAAGTCg acaatcaacc tctggattac
1741aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.