Construct: ORF TRCN0000492015
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018985.1_s317c1
- Derived from:
- ccsbBroadEn_15077
- DNA Barcode:
- CTTCTATTCAAAGCCTATCACCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKAG3 (53632)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492015
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53632 | PRKAG3 | protein kinase AMP-activate... | NM_017431.3 | 99.2% | 48.6% | (many diffs) |
| 2 | human | 53632 | PRKAG3 | protein kinase AMP-activate... | XM_017004343.2 | 99.2% | 48.6% | (many diffs) |
| 3 | mouse | 241113 | Prkag3 | protein kinase, AMP-activat... | NM_153744.3 | 84.9% | 35.3% | (many diffs) |
| 4 | mouse | 241113 | Prkag3 | protein kinase, AMP-activat... | XM_006495988.3 | 84.9% | 35.3% | (many diffs) |
| 5 | mouse | 241113 | Prkag3 | protein kinase, AMP-activat... | XM_006495989.3 | 84.9% | 35.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 801
- ORF length:
- 732
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcccggg ctggagcacg cactgcgcag gaccccttcc tggagcagcc 121 ttgggggttc tgagcatcaa gagatgagct tcctagagca agaaaacagc agctcatggc 181 catcaccagc tgtgaccagc agctcagaaa gaatccgtgg gaaacggagg gccaaagcct 241 tgagatggac aaggcagaag tcggtggagg aaggggagcc accaggtcag ggggaaggtc 301 cccggtccag gccagctgct gagtccaccg ggctggaggc cacattcccc aagaccacac 361 ccttggctca agctgatcct gccggggtgg gcactccacc aacagggtgg gactgcctcc 421 cctctgactg tacagcctca gctgcaggct ccagcacaga tgatgtggag ctggccacgg 481 agttcccagc cacagaggcc tgggagtgtg agctagaagg cctgctggaa gagaggcctg 541 ccctgtgcct gtccccgcag gccccatttc ccaagctggg ctgggatgac gaactgcgga 601 aacccggcgc ccagatctac atgcgcttca tgcaggagca cacctgctac gatgccatgg 661 caactagctc caagctagtc atcttcgaca ccatgctgga gatcaagaag gccttctttg 721 ctctggtggc caacggggtg cgggcagccc ctctatggga cagcaagaag cagagctttt 781 gtggggatgc tgaccatcac tgactttttc ctggtgcggc atcgttattc caggtccccc 841 cttgtcccag atctatgaga ttgaacaaca taagattgag acctggaggg agatctacct 901 gcaaggctgc ttcaagcttc tggtctccat ctctcctaat gatagcctgt ttgaagctgt 961 ctacaccctc atcaagaacc ggatccatcg cctgcctgtt cttgacccgg tgtcaggcaa 1021 cgtactccac atcctcacac acaaacgcct gctcaagttc ctgcacatct ttggttccct 1081 gctgccccgg ccctccttcc tctaccgcac tatccaagat ttgggcatcg gcacattccg 1141 agacttggct gtggtgctGG AGACAGCACC CATCCTGACT GCACTGGACA TCTTTGTGGA 1201 CCGGCGTGTG TCTGCACTGC CTGTGGTCAA CGAATGTGGT CAGGTCGTGG GCCTCTATTC 1261 CCGCTTTGAT GTGATTCACC TGGCTGCCCA GCAAACCTAC AACCACCTGG ACATGAGTGT 1321 GGGAGAAGCC CTGAGGCAGA GGACACTATG TCTGGAGGGA GTCCTTTCCT GCCAGCCCCA 1381 CGAGAGCTTG GGGGAAGTGA TCGACAGGAT TGCTCGGGAG CAGGTACACA GGCTGGTGCT 1441 AGTGGACGAG ACCCAGCATC TCTTGGGCGT GGTCTCCCTC TCCGACATCC TTCAGGCACT 1501 GGTGCTCAGC CCTGCTGGCA TCGATGCCCT CGGGGCCTTG CCAACTTTCT TGTACAAAGT 1561 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 1621 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 1681 CGACTTCTAT TCAAAGCCTA TCACCACACG CGTTAAGTCg acaatcaacc tctggattac 1741 aaaatttgtg aaagatt