Construct: ORF TRCN0000492028

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019836.2_s317c1
DNA Barcode:
GACGGTAGGTTTTTAAAGGTTAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
USP54 (159195)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492028
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 159195 USP54 ubiquitin specific peptidas... XM_005269582.2 45.5% 45.5% 1_2565del;3985_3999del;4740_4741insG
2 human 159195 USP54 ubiquitin specific peptidas... NM_001350995.2 45.3% 45.3% 1_2445del;3865_4020del;4761_4762insG
3 human 159195 USP54 ubiquitin specific peptidas... XM_024447852.1 45.3% 45.3% 1_2445del;3865_4020del;4761_4762insG
4 human 159195 USP54 ubiquitin specific peptidas... XM_024447851.1 44.4% 44.4% 1_2682del;4102_4116del;4857_4858insG
5 human 159195 USP54 ubiquitin specific peptidas... NM_001320437.2 44.3% 44.3% 1_2565del;3985_4125del;4866_4867insG
6 human 159195 USP54 ubiquitin specific peptidas... XM_017015783.1 44.2% 44.2% 1_2565del;3985_4140del;4881_4882insG
7 human 159195 USP54 ubiquitin specific peptidas... XM_017015784.1 44.2% 44.2% 1_2565del;3985_4140del;4881_4882insG
8 human 159195 USP54 ubiquitin specific peptidas... XM_017015782.2 43.7% 43.7% 1_2616del;4036_4191del;4932_4933insG
9 human 159195 USP54 ubiquitin specific peptidas... XM_011539368.2 43.6% 43.6% 1_2631del;4051_4206del;4947_4948insG
10 human 159195 USP54 ubiquitin specific peptidas... XM_024447850.1 43.5% 43.5% 1_2802del;4962_4963insG
11 human 159195 USP54 ubiquitin specific peptidas... XM_024447849.1 43.3% 43.3% 1_2802del;4222_4236del;4977_4978insG
12 human 159195 USP54 ubiquitin specific peptidas... XM_024447848.1 43.2% 43.1% 1_2682del;4102_4257del;4998_4999insG
13 human 159195 USP54 ubiquitin specific peptidas... NM_152586.3 42.7% 42.7% 1_2736del;4156_4311del;5052_5053insG
14 human 159195 USP54 ubiquitin specific peptidas... XM_017015774.1 42.7% 42.7% 1_2736del;4156_4311del;5052_5053insG
15 human 159195 USP54 ubiquitin specific peptidas... XM_017015775.1 42.7% 42.7% 1_2736del;4156_4311del;5052_5053insG
16 human 159195 USP54 ubiquitin specific peptidas... XM_017015777.1 42.7% 42.7% 1_2736del;4156_4311del;5052_5053insG
17 human 159195 USP54 ubiquitin specific peptidas... XM_024447847.1 42.7% 42.7% 1_2736del;4156_4311del;5052_5053insG
18 human 159195 USP54 ubiquitin specific peptidas... XM_024447846.1 42.3% 42.3% 1_2802del;4222_4362del;5103_5104insG
19 human 159195 USP54 ubiquitin specific peptidas... XM_024447832.1 42.1% 42.1% 1_2802del;4222_4377del;5118_5119insG
20 human 159195 USP54 ubiquitin specific peptidas... XM_024447833.1 42.1% 42.1% 1_2802del;4222_4377del;5118_5119insG
21 human 159195 USP54 ubiquitin specific peptidas... XM_024447834.1 42.1% 42.1% 1_2802del;4222_4377del;5118_5119insG
22 human 159195 USP54 ubiquitin specific peptidas... XM_024447835.1 42.1% 42.1% 1_2802del;4222_4377del;5118_5119insG
23 human 159195 USP54 ubiquitin specific peptidas... XM_024447836.1 42.1% 42.1% 1_2802del;4222_4377del;5118_5119insG
24 human 159195 USP54 ubiquitin specific peptidas... XM_024447837.1 42.1% 42.1% 1_2802del;4222_4377del;5118_5119insG
25 human 159195 USP54 ubiquitin specific peptidas... XM_024447838.1 42.1% 42.1% 1_2802del;4222_4377del;5118_5119insG
26 human 159195 USP54 ubiquitin specific peptidas... XM_024447839.1 42.1% 42.1% 1_2802del;4222_4377del;5118_5119insG
27 human 159195 USP54 ubiquitin specific peptidas... XM_024447840.1 42.1% 42.1% 1_2802del;4222_4377del;5118_5119insG
28 human 159195 USP54 ubiquitin specific peptidas... XM_024447841.1 42.1% 42.1% 1_2802del;4222_4377del;5118_5119insG
29 human 159195 USP54 ubiquitin specific peptidas... XM_024447842.1 42.1% 42.1% 1_2802del;4222_4377del;5118_5119insG
30 human 159195 USP54 ubiquitin specific peptidas... XM_024447843.1 42.1% 42.1% 1_2802del;4222_4377del;5118_5119insG
31 human 159195 USP54 ubiquitin specific peptidas... XM_024447844.1 42.1% 42.1% 1_2802del;4222_4377del;5118_5119insG
32 human 159195 USP54 ubiquitin specific peptidas... XM_024447845.1 42.1% 42.1% 1_2802del;4222_4377del;5118_5119insG
33 human 159195 USP54 ubiquitin specific peptidas... NR_135250.1 38.7% 1_2135del;3555_3695del;4437_5569delinsG
34 human 159195 USP54 ubiquitin specific peptidas... XR_001747048.1 37.6% 1_2331del;3751_3906del;4648_5741delinsG
35 human 159195 USP54 ubiquitin specific peptidas... XR_001747042.1 36.5% 1_2502del;3922_4077del;4819_5912delinsG
36 human 159195 USP54 ubiquitin specific peptidas... NR_146998.2 36% 1_2728del;4148_4162del;4904_5997delinsG
37 human 159195 USP54 ubiquitin specific peptidas... NR_146997.2 35.1% 1_2728del;4148_4303del;5045_6138delinsG
38 human 159195 USP54 ubiquitin specific peptidas... NR_135249.2 33.7% 1_3132del;4552_4566del;5308_6401delinsG
39 human 159195 USP54 ubiquitin specific peptidas... XR_001747041.2 31.6% 1_3407del;4827_4982del;5724_6817delinsG
40 human 159195 USP54 ubiquitin specific peptidas... XR_001747037.2 28.7% 1_4093del;5513_5668del;6410_7503delinsG
41 human 159195 USP54 ubiquitin specific peptidas... XR_001747046.1 27.7% 1_4385del;5805_5960del;6702_7795delinsG
42 human 159195 USP54 ubiquitin specific peptidas... XR_001747043.1 27.6% 1_4556del;5976_5990del;6732_7825delinsG
43 human 159195 USP54 ubiquitin specific peptidas... XR_001747035.1 26.8% 1_4622del;6042_6197del;6939_8032delinsG
44 human 159195 USP54 ubiquitin specific peptidas... XR_001747038.1 25.5% 1_5055del;6475_6630del;7372_8465delinsG
45 human 159195 USP54 ubiquitin specific peptidas... XR_001747047.1 24.2% 1_5515del;6935_7075del;7817_8910delinsG
46 human 159195 USP54 ubiquitin specific peptidas... XR_001747044.1 24.1% 1_5686del;7847_8940delinsG
47 human 159195 USP54 ubiquitin specific peptidas... XR_001747039.1 24.1% 1_5686del;7106_7120del;7862_8955delinsG
48 human 159195 USP54 ubiquitin specific peptidas... XR_002956962.1 24% 1_5581del;7001_7156del;7898_8991delinsG
49 human 159195 USP54 ubiquitin specific peptidas... XR_001747036.1 23.9% 1_5752del;7172_7186del;7928_9021delinsG
50 human 159195 USP54 ubiquitin specific peptidas... XR_002956959.1 23.5% 1_5752del;7172_7327del;8069_9162delinsG
51 human 159195 USP54 ubiquitin specific peptidas... XR_002956960.1 20.6% 1_5752del;7386_7387ins527
52 human 159195 USP54 ubiquitin specific peptidas... XR_002956961.1 18% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2232
ORF length:
2163
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat ggatacagag tttggggcca gttctttctt ccattcacct gcttcctgcc
121atgagtcaca ctcatcacta tctccagagt catctgcccc acagcacagc tcccccagta
181gatctgcctt gaagcttctg acttcggttg aagtagacaa cattgaaccc tctgcattcc
241acaggcaagg tttacctaaa gcaccagggt ggactgagaa gaattctcat catagttggg
301agccattgga tgccccagag ggtaagctgc aaggctctag gtgtgacaac agcagttgca
361gcaagctccc tccacaagaa ggaagaggca ttgctcaaga acagctgttc caagaaaaga
421aggatcctgc taacccctcc ccggtgatgc ctggaatagc cacctctgag aggggtgatg
481aacacagcct aggctgtagt ccttcaaatt catcagctca gcccagcctt cccctgtata
541gaacctgcca ccccataatg cctgttgctt cttcatttgt gcttcactgt cctgatcctg
601tgcagaaaac taaccaatgc ctccaaggcc aaagcctcaa aacttcattg actttaaaag
661tggacagagg cagtgaggag acctataggc cagagtttcc cagcacaaag gggcttgtcc
721gttctctggc tgagcagttc cagaggatgc agggtgtctc catgagggat agtacaggtt
781tcaaggatag aagtttgtca ggtagtctaa ggaagaactc ttccccttct gattctaagc
841ctcctttctc acagggtcaa gagaaaggcc actggccatg ggcaaagcaa caatcctctc
901tggagggtgg ggatagacca ctttcctggg aagagtccac tgaacattct tctcttgcct
961taaactctgg gctgcctaat ggtgaaactt ctagcggagg acagcccagg ttggcagagc
1021cagacatata ccaagagaag ctgtcccaag tgagagatgt taggtctaag gatctgggca
1081gcagtactga cttggggact tccttgcctt tggattcctg ggtgaatatc acaaggttct
1141gtgattctca gcttaagcat ggggcaccta ggccaggaat gaagtcctcc cctcatgatt
1201cccatacgtg tgtaacctat ccagagagaa atcacatcct tttgcatcca cattggaacc
1261aagacacaga gcaggagacc tcagaattgg agtctctgta tcaggccagt cttcaggctt
1321ctcaagctgg ctgttctgga tgggggcagc aggataccgc ctggcaccca cttagccaaa
1381caggctctgc agatggcatg gggaggaggt tgcactcagc ccatgatcct ggtctctcaa
1441agacttcaac agcagaaatg gagcatggtc tccatgaagc cagaacagat tcatcaaacg
1501tgaggaagcc tttggaaacc gggcaccgtt gttccagctc ctcttccctc cctgtcatcc
1561atgacccttc tgtgtttctc ctcggtcccc aactctacct tccccaacca cagttcctgt
1621ccccagatgt cctgatgccc accatggcag gggagcccaa tagactccca ggaacttcaa
1681ggagtgtcca gcagtttctg gctatgtgtg acaggggtga aacttcccaa ggggccaagt
1741acacaggaag gactttgaac taccagagcc tcccccatcg ctccagaaca gacaactcct
1801gggcaccctg gtcagagacc aaccagcata ttgggaccag attcctgacT ACTCCAGGGT
1861GCAATCCTCA ACTAACCTAC ACTGCCACAC TACCAGAAAG AAGCAAGGGC CTTCAGGTTC
1921CTCACACTCA GTCCTGGAGT GATCTTTTCC ATTCACCCTC CCACCCTCCC ATTGTTCATC
1981CTGTGTACCC ACCATCTAGC AGTCTTCATG TACCCCTGAG GTCAGCTTGG AATTCAGATC
2041CTGTTCCAGG GTCCCGAACC CCTGGTCCTC GAAGAGTAGA TATGCCCCCA GATGATGACT
2101GGAGGCAAAG CAGTTATGCC TCCCACTCTG GACACAGGAG AACAGTGGGA GAGGGGTTTC
2161TGTTTGTTCT ATCAGATGCT CCCAGAAGAG AGCAGATCAG GGCTAGAGTC CTGCAGCACA
2221GTCAATGGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
2281CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
2341TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGACGGT AGGTTTTTAA AGGTTAATAC
2401GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)