Construct: ORF TRCN0000492035
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016314.1_s317c1
- Derived from:
- ccsbBroadEn_12484
- DNA Barcode:
- TACGCTATCTCAAATTAAGCATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAREM1 (64762)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492035
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64762 | GAREM1 | GRB2 associated regulator o... | NM_001242409.2 | 92.1% | 92.1% | 1482_1688del |
| 2 | human | 64762 | GAREM1 | GRB2 associated regulator o... | NM_022751.3 | 92% | 92% | 1482_1688del;1733_1734insCAG |
| 3 | human | 64762 | GAREM1 | GRB2 associated regulator o... | XM_024451234.1 | 78.1% | 78.1% | 0_1ins366;1116_1322del |
| 4 | human | 64762 | GAREM1 | GRB2 associated regulator o... | XM_024451235.1 | 78.1% | 78.1% | 0_1ins366;1116_1322del |
| 5 | human | 64762 | GAREM1 | GRB2 associated regulator o... | XM_024451236.1 | 78% | 78% | 0_1ins366;1116_1322del;1367_1368insCAG |
| 6 | human | 64762 | GAREM1 | GRB2 associated regulator o... | XM_017025919.1 | 73.6% | 65.5% | (many diffs) |
| 7 | mouse | 381126 | Garem1 | GRB2 associated regulator o... | NM_001033445.2 | 81.1% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2490
- ORF length:
- 2421
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacccggcg ccctcgctgg gctgcagcct caaggatgtg aagtggagct 121 cggtggccgt gccgctcgac ctcctggtca gcacttaccg gctgccccag atcgcgcgcc 181 tggacaacgg agagtgcgta gaagggctgc gggaaaatga ctatctgctg attcattcct 241 gccgccagtg gaccaccatc actgcccaca gcttggagga gggtcactat gtcattgggc 301 caaagataga gattccggta cattatgcag ggcaattcaa gctgctggaa caagaccgag 361 atataaagga gccagtgcaa tatttcaaca gtgtggagga ggtggctaag gcatttcctg 421 aacgcgtgta cgtcatggag gatatcacat tcaacgtgaa ggttgcttca ggtgaatgca 481 atgaagacac tgaagtttac aacatcaccc tgtgtactgg ggatgaactc actctaatgg 541 ggcaggcaga aatcctttat gcaaagacat tcaaggaaaa gtcacgactc aacacaatct 601 tcaaaaagat tgggaagctc aattccatca gcaagctggg aaaaggcaaa atgccgtgcc 661 tcatttgtat gaatcaccgg accaacgaaa gcattagcct tccattccag tgcaagggca 721 gatttagcac ccgaagtccc ctggaacttc agatgcaaga gggcgaacac accatccgca 781 acattgtgga gaaaaccagg cttcctgtga atgtgactgt gccaagccct ccaccgagaa 841 acccatacga cctccacttc atccgtgagg ggcaccgcta taagtttgtg aacatccaga 901 ccaagacggt ggtggtttgc tgtgtgctgc ggaacaacaa gatcctcccc atgcactttc 961 ctttgcactt gactgtcccc aagttcagcc tcccagaaca cctggtgaag ggagagagct 1021 ggcccgaaac cctggtccat cactggctag gtatctgcca agaacagttc gacatcgatg 1081 agtattcacg ggctgtccgt gatgtgaaaa ccgactggaa tgaagaatgc aagagcccca 1141 agaagggtcg gtgctctggc cacaaccacg tgcccaattc gctcagctac gcccgcgatg 1201 agctcaccca gtccttccac cgactctcgg tctgtgtgta tggcaacaat ctccatggca 1261 acagtgaggt gaaccttcat ggttgcaggg acctgggggg agattgggct ccctttcctc 1321 atgacatcct gccctatcag gactctggag atagtgggag cgactacctt ttcccagaag 1381 ctagtgaaga atcagcaggc atcccgggaa agtcagaact tccctacgaa gagctgtggc 1441 tggaggaagg caagcccagc catcagcctc tcactcgctc tctgagcgag aagaacagat 1501 gtgatcagtt tagaggttct gtccgatcca aatgtgcgac ttctcctctt cccagcccca 1561 ccttgtccta ctattcttca gggctacaca acatcagcgt cactaaaact gacacaaatc 1621 cttctgaaag cactcctgtt tcctgctatc catgtaaccg agtgaaaact gattctgtgg 1681 acctgaaatc cccgtttgga agtccttctg ctgaagctgt gtcctctcgg ctctcatggc 1741 ctaaccatta ttcaggagca tcagaaagcc agaccaggag tgacttcctg ctggatccaa 1801 gcaggagtta tagttaccct agacaaaaga cgccaggcac accaaagaga aactgcccag 1861 caccttttga ttttgatggc tgtgagctcc tggccagccc cactagccca gtcactgcag 1921 aattcagtag cagcgtctct ggttgtccca agtcagccag ctactctctg gagagcacag 1981 atgtgaaatc tcttgcagct ggtgtgacaa agcagagtac gtcatgccct gccttacccc 2041 ccagggctcc aaaactagtg gaagagaagg tcgcctccga aacatctcct ttgcctctga 2101 aaattgatgg tgctgaggaa gaccccaagt ctgggtcacc agatctctcG GAGGACCAGT 2161 ATTTTGTTAA AAAGGGCATG CAGGACATCT TCTCTGCCTC CTACCCTTTC TCATCTCCGC 2221 TCCATCTCCA GCTGGCCCCC AGATCCTGTG GCGACGGTTC CCCATGGCAG CCACCTGCTG 2281 ACCTATCAGG ACTCTCTATA GAGGAAGTGT CCAAGTCACT ACGGTTCATT GGTTTGTCCG 2341 AAGATGTCAT ATCATTCTTT GTTACTGAAA AGATTGATGG GAACCTGCTT GTTCAGCTAA 2401 CGGAAGAAAT CCTCTCAGAG GATTTCAAAT TGAGCAAATT GCAGGTGAAG AAGATAATGC 2461 AATTCATTAA TGGCTGGAGG CCCAAAATAT TGCCAACTTT CTTGTACAAA GTGGTTGATA 2521 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2581 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATACGC 2641 TATCTCAAAT TAAGCATGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2701 tgaaagatt