Construct: ORF TRCN0000492047
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002053.2_s317c1
- Derived from:
- ccsbBroadEn_02484
- DNA Barcode:
- GCATTTTAACATCCAACACCCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAICS (10606)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492047
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10606 | PAICS | phosphoribosylaminoimidazol... | NM_001079524.2 | 100% | 100% | |
| 2 | human | 10606 | PAICS | phosphoribosylaminoimidazol... | NM_006452.3 | 100% | 100% | |
| 3 | human | 10606 | PAICS | phosphoribosylaminoimidazol... | NM_001079525.2 | 98.3% | 98.1% | 215_235del |
| 4 | human | 10606 | PAICS | phosphoribosylaminoimidazol... | XM_011534366.1 | 87.5% | 87.5% | 951_952ins159 |
| 5 | mouse | 67054 | Paics | phosphoribosylaminoimidazol... | NM_001356971.1 | 87.8% | 95% | (many diffs) |
| 6 | mouse | 67054 | Paics | phosphoribosylaminoimidazol... | NM_001356972.1 | 87.8% | 95% | (many diffs) |
| 7 | mouse | 67054 | Paics | phosphoribosylaminoimidazol... | NM_001356973.1 | 87.8% | 95% | (many diffs) |
| 8 | mouse | 67054 | Paics | phosphoribosylaminoimidazol... | NM_025939.3 | 87.8% | 95% | (many diffs) |
| 9 | mouse | 67054 | Paics | phosphoribosylaminoimidazol... | XM_006535183.4 | 87.8% | 95% | (many diffs) |
| 10 | mouse | 67054 | Paics | phosphoribosylaminoimidazol... | NM_001356974.1 | 87.7% | 95% | (many diffs) |
| 11 | mouse | 67054 | Paics | phosphoribosylaminoimidazol... | XM_006535184.2 | 87.7% | 95% | (many diffs) |
| 12 | mouse | 67054 | Paics | phosphoribosylaminoimidazol... | XM_006535185.4 | 87.7% | 95% | (many diffs) |
| 13 | mouse | 67054 | Paics | phosphoribosylaminoimidazol... | XM_017321075.2 | 87.7% | 95% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1341
- ORF length:
- 1275
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gacagctgag gtactgaaca ttggtaaaaa attatatgag ggtaaaacaa 121 aagaagtcta cgaattgtta gacagtccag gaaaagtcct cctgcagtcc aaggaccaga 181 ttacagcagg aaatgcagct agaaaaaacc acctggaagg aaaagctgca atctcaaata 241 aaatcaccag ttgtattttt cagttattac aggaagcagg tattaaaact gccttcacca 301 gaaaatgtgg ggagacagct ttcattgcac cgcagtgtga aatgattcca attgaatggg 361 tttgcagaag aatagcaact ggttcttttc tcaaaagaaa tcctggtgtc aaggaaggat 421 ataagtttta cccacctaaa gtggagttgt ttttcaagga tgatgccaat aatgacccac 481 agtggtctga ggaacagctg attgctgcaa aattttgctt tgctggactt cttataggcc 541 agactgaagt ggatatcatg agtcatgcta cacaggctat atttgaaata ctggagaaat 601 cctggttgcc ccagaattgt acactggttg atatgaagat tgaatttggt gttgatgtaa 661 ccaccaaaga aattgttctt gctgatgtta ttgacaatga ttcctggaga ctctggccat 721 caggagatcg aagccaacag aaagacaaac agtcttatcg ggacctcaaa gaagtaactc 781 ctgaagggct ccaaatggta aagaaaaact ttgagtgggt tgcagagaga gtagagttgc 841 ttttgaaatc agaaagtcag tgcagggttg tagtgttgat gggctctact tctgatcttg 901 gtcactgtga aaaaatcaag aaggcctgtg gaaattttgg cattccatgt gaacttcgag 961 taacatcTGC GCATAAAGGA CCAGATGAAA CTCTGAGGAT TAAAGCTGAG TATGAAGGGG 1021 ATGGCATTCC TACTGTATTT GTGGCAGTGG CAGGCAGAAG TAATGGTTTG GGACCAGTGA 1081 TGTCTGGGAA CACTGCATAT CCAGTTATCA GCTGTCCTCC CCTCACACCA GACTGGGGAG 1141 TTCAGGATGT GTGGTCTTCT CTTCGACTAC CCAGTGGTCT TGGCTGTTCA ACCGTACTTT 1201 CTCCAGAAGG ATCAGCTCAA TTTGCTGCTC AGATATTTGG GTTAAGCAAC CATTTGGTAT 1261 GGAGCAAACT GCGAGCAAGC ATTTTGAACA CATGGATTTC CTTGAAGCAG GCTGACAAGA 1321 AAATCAGAGA ATGTAATTTA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1381 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1441 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCAT TTTAACATCC 1501 AACACCCTAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt