Transcript: Human NM_001079525.2

Homo sapiens phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthase (PAICS), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
PAICS (10606)
Length:
6392
CDS:
54..1352

Additional Resources:

NCBI RefSeq record:
NM_001079525.2
NBCI Gene record:
PAICS (10606)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001079525.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000293753 TCGACTAACAGGGACTATAAT pLKO_005 1827 3UTR 100% 15.000 21.000 N PAICS n/a
2 TRCN0000045774 CGCAGTGTGAAATGATTCCAA pLKO.1 340 CDS 100% 3.000 4.200 N PAICS n/a
3 TRCN0000286312 CGCAGTGTGAAATGATTCCAA pLKO_005 340 CDS 100% 3.000 4.200 N PAICS n/a
4 TRCN0000045776 CGCATAAAGGACCAGATGAAA pLKO.1 979 CDS 100% 5.625 4.500 N PAICS n/a
5 TRCN0000045777 GACCAGATTACAGCAGGAAAT pLKO.1 162 CDS 100% 10.800 7.560 N PAICS n/a
6 TRCN0000286243 GACCAGATTACAGCAGGAAAT pLKO_005 162 CDS 100% 10.800 7.560 N PAICS n/a
7 TRCN0000045775 GCTGCTCAGATATTTGGGTTA pLKO.1 1233 CDS 100% 4.050 2.835 N PAICS n/a
8 TRCN0000286311 GCTGCTCAGATATTTGGGTTA pLKO_005 1233 CDS 100% 4.050 2.835 N PAICS n/a
9 TRCN0000076101 CTGCTCAGATATTTGGGTTAA pLKO.1 1234 CDS 100% 10.800 6.480 N Paics n/a
10 TRCN0000363773 CTGCTCAGATATTTGGGTTAA pLKO_005 1234 CDS 100% 10.800 6.480 N Paics n/a
11 TRCN0000045773 CCCACCTAAAGTGGAGTTGTT pLKO.1 440 CDS 100% 4.950 2.970 N PAICS n/a
12 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 2600 3UTR 100% 4.950 2.475 Y ORAI2 n/a
13 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 5245 3UTR 100% 4.950 2.475 Y ERAP2 n/a
14 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 2561 3UTR 100% 4.050 2.025 Y P3H4 n/a
15 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 2561 3UTR 100% 4.050 2.025 Y ORAI2 n/a
16 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 2561 3UTR 100% 4.050 2.025 Y P3H4 n/a
17 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 4553 3UTR 100% 13.200 6.600 Y LIAS n/a
18 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 2597 3UTR 100% 4.950 2.475 Y LOC339059 n/a
19 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 4718 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001079525.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02484 pDONR223 100% 98.3% 98.1% None 215_235del n/a
2 ccsbBroad304_02484 pLX_304 0% 98.3% 98.1% V5 215_235del n/a
3 TRCN0000492047 GCATTTTAACATCCAACACCCTAC pLX_317 29.6% 98.3% 98.1% V5 215_235del n/a
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