Construct: ORF TRCN0000492051
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008153.1_s317c1
- Derived from:
- ccsbBroadEn_09243
- DNA Barcode:
- GACTTTTCTTACCTTTTACAGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MEDAG (84935)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492051
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84935 | MEDAG | mesenteric estrogen depende... | NM_032849.4 | 99.8% | 99.6% | 175A>G |
| 2 | human | 84935 | MEDAG | mesenteric estrogen depende... | XM_017020801.1 | 50.1% | 50.1% | 0_1ins453 |
| 3 | mouse | 70717 | Medag | mesenteric estrogen depende... | NM_027519.3 | 86.2% | 91.4% | (many diffs) |
| 4 | mouse | 70717 | Medag | mesenteric estrogen depende... | XM_006504940.3 | 60.2% | 63.3% | (many diffs) |
| 5 | mouse | 70717 | Medag | mesenteric estrogen depende... | XM_006504941.2 | 60.2% | 63.3% | (many diffs) |
| 6 | mouse | 70717 | Medag | mesenteric estrogen depende... | XM_006504942.3 | 53.2% | 56.1% | (many diffs) |
| 7 | mouse | 70717 | Medag | mesenteric estrogen depende... | XR_001784745.1 | 21.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 975
- ORF length:
- 909
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggggcggcc tgcgagccgg tggccaggcc gagcctgacc tccatctcgt 121 ctggggagct tcgcagcctg tggacctgcg actgcgagct ggccctgctg ccgctggctc 181 agctgctgcg cctgcagccc ggtgccttcc agctgagcgg cgaccagctc gtggtggccg 241 ggcccgggga gccggcggcg gcgcgggggg gcttcaacgt cttcggtgac ggcctcgtgc 301 gcctcgacgg gcagctctac cgcctcagca gctacatcaa gaggtatgtg gaactgacca 361 actactgtga ttataaagac tacagggaaa ctatattgag caaaccaatg ttgttcttta 421 ttaatgtaca gaccaaaaaa gacacctcaa aagaaaggac gtacgcgttt cttgtaaaca 481 cgaggcaccc caagataaga agacagatag agcaagggat ggacatggtc atctcctcag 541 tgattGGAGA AAGTTACCGG CTTCAGTTTG ATTTTCAAGA GGCAGTGAAG AATTTCTTCC 601 CCCCAGGAAA TGAAGTGGTT AATGGAGAAA ATTTAAGCTT TGCATATGAA TTCAAAGCTG 661 ATGCATTATT TGATTTCTTC TATTGGTTTG GGCTCAGTAA TTCCGTTGTA AAAGTAAATG 721 GAAAAGTTCT GAATTTGTCA AGTACAAGTC CAGAAAAGAA GGAGACGATT AAGTTATTTC 781 TGGAAAAAAT GAGTGAGCCT TTAATCCGAA GGAGCAGTTT CTCTGACCGA AAGTTCAGTG 841 TAACTTCCAG AGGTTCAATA GATGATGTTT TTAACTGCAA TCTGTCACCC AGATCATCTC 901 TGACAGAGCC TCTTTTGGCA GAATTACCAT TTCCAAGTGT TCTGGAATCT GAAGAGACAC 961 CCAACCAATT TATCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1021 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1081 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GACTTTTCTT ACCTTTTACA 1141 GAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt