Construct: ORF TRCN0000492057

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012100.1_s317c1
Derived from:
ccsbBroadEn_08590
DNA Barcode:
AAGTTTCTGTGCTCACCTTCCAGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CACNA2D3 (55799)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492057
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55799 CACNA2D3 calcium voltage-gated chann... NM_018398.3 99.9% 100% 2265C>T
2 mouse 12294 Cacna2d3 calcium channel, voltage-de... NM_009785.1 89.9% 98% (many diffs)
3 mouse 12294 Cacna2d3 calcium channel, voltage-de... XM_006518486.4 89.5% 97.5% (many diffs)
4 mouse 12294 Cacna2d3 calcium channel, voltage-de... XM_011244938.3 87.5% 95.3% (many diffs)
5 mouse 12294 Cacna2d3 calcium channel, voltage-de... XM_030247616.1 82.1% 89.9% (many diffs)
6 mouse 12294 Cacna2d3 calcium channel, voltage-de... XR_001781093.2 46.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3342
ORF length:
3273
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggccgggccg ggctcgccgc gccgcgcgtc ccggggggcc tcggcgcttc
121tcgctgccgc gcttctctac gccgcgctgg gggacgtggt gcgctcggag cagcagatac
181cgctctccgt ggtgaagctc tgggcctcgg cttttggtgg ggagataaaa tccattgctg
241ctaagtactc cggttcccag cttctgcaaa agaaatacaa agagtatgag aaagacgttg
301ccatagaaga aattgatggc ctccaactgg taaagaagct ggcaaagaac atggaagaga
361tgtttcacaa gaagtctgag gccgtcaggc gtctggtgga ggctgcagaa gaagcacacc
421tgaaacatga atttgatgca gacttacagt atgaatactt caatgctgtg ctgataaatg
481aaagggacaa agacgggaat tttttggagc tgggaaagga attcatctta gccccaaatg
541accattttaa taatttgcct gtgaacatca gtctaagtga cgtccaagta ccaacgaaca
601tgtacaacaa agaccctgca attgtcaatg gggtttattg gtctgaatct ctaaacaaag
661tttttgtaga taactttgac cgtgacccat ctctcatatg gcagtacttt ggaagtgcaa
721agggcttttt taggcagtat ccggggatta aatgggaacc agatgagaat ggagtcattg
781ccttcgactg caggaaccga aaatggtaca tccaggcagc aacttctccg aaagacgtgg
841tcattttagt tgacgtcagt ggcagcatga aaggactccg tctgactatc gcgaagcaaa
901cagtctcatc cattttggat acacttgggg atgatgactt cttcaacata attgcttata
961atgaggagct tcactatgtg gaaccttgcc tgaatggaac tttggtgcaa gccgacagga
1021caaacaaaga gcacttcagg gagcatctgg acaaactttt cgccaaagga attggaatgt
1081tggatatagc tctgaatgag gccttcaaca ttctgagtga tttcaaccac acgggacaag
1141gaagtatctg cagtcaggcc atcatgctca taactgatgg ggcggtggac acctatgata
1201caatctttgc aaaatacaat tggccagatc gaaaggttcg catcttcaca tacctcattg
1261gacgagaggc tgcgtttgca gacaatctaa agtggatggc ctgtgccaac aaaggatttt
1321ttacccagat ctccaccttg gctgatgtgc aggagaatgt catggaatac cttcacgtgc
1381ttagccggcc caaagtcatc gaccaggagc atgatgtggt gtggaccgaa gcttacattg
1441acagcactct ccctcaggca caaaagctga ctgatgatca gggccccgtc ctgatgacca
1501ctgtagccat gcctgtgttt agtaagcaga acgaaaccag atcgaagggc attcttctgg
1561gagtggttgg cacagatgtc ccagtgaaag aacttctgaa gaccatcccc aaatacaagt
1621tagggattca cggttatgcc tttgcaatca caaataatgg atatatcctg acgcatccgg
1681aactcaggct gctgtacgaa gaaggaaaaa agcgaaggaa acctaactat agtagcgttg
1741acctctctga ggtggagtgg gaagaccgag atgacgtgtt gagaaatgct atggtgaatc
1801gaaagacggg gaagttttcc atggaggtga agaagacagt ggacaaaggg aaacgggttt
1861tggtgatgac aaatgactac tattatacag acatcaaggg tactcctttc agtttaggtg
1921tggcgctttc cagaggtcat gggaaatatt tcttccgagg gaatgtaacc atcgaagaag
1981gcctgcatga cttagaacat cccgatgtgt ccttggcaga tgaatggtcc tactgcaaca
2041ctgacctaca ccctgagcac cgccatctgt ctcagttaga agcgattaag ctctacctaa
2101aaggcaaaga acctctgctc cagtgtgata aagaattgat ccaagaagtc ctttttgacg
2161cggtggtgag tgcccccatt gaagcgtatt ggaccagcct ggccctcaac aaatctgaaa
2221attctgacaa gggcgtggag gttgccttcc tcggcactcg cacgggcctc tccagaatca
2281acctgtttgt cggggctgag cagctcacca atcaggactt cctgaaagct ggtgacaagg
2341agaacatttt taacgcagac catttccctc tctggtaccg aagagccgct gagcagattc
2401cagggagctt cgtctactcg atcccattca gcactggacc agtcaataaa agcaatgtgg
2461tgacagcaag tacatccatc cagctcctgg atgaacggaa atctcctgtg gtggcagctg
2521taggcattca gatgaaactt gaatttttcc aaaggaagtt ctggactgcc agcagacagt
2581gtgcttccct ggatggcaaa tgctccatca gctgtgatga tgagactgtg aattgttacc
2641tcatagacaa taatggattt attttggtgt ctgaagacta cacacagact ggagactttt
2701ttggtgagat cgagggagct gtgatgaaca aattgctaac aatgggctcc tttaaaagaa
2761ttacccttta tgactaccaa gccatgtgta gagccaacaa ggaaagcagc gatggcgccc
2821atggcctcct ggatccttat aatgccttcc tctctgcagt aaaatggatc atgacagaac
2881ttgtcttgtt cctggtggaa tttaacctct gcagttggtg gcactccgat atgacagcta
2941aagcccagaa attgaaacag accctggagc cttgtgatac tgaatatcca gcattcgtct
3001cTGAGCGCAC CATCAAGGAG ACTACAGGGA ATATTGCTTG TGAAGACTGC TCCAAGTCCT
3061TTGTCATCCA GCAAATCCCA AGCAGCAACC TGTTCATGGT GGTGGTGGAC AGCAGCTGCC
3121TCTGTGAATC TGTGGCCCCC ATCACCATGG CACCCATTGA AATCAGGTAT AATGAATCCC
3181TTAAGTGTGA ACGTCTAAAG GCCCAGAAGA TCAGAAGGCG CCCAGAATCT TGTCATGGCT
3241TCCATCCTGA GGAGAATGCA AGGGAGTGTG GGGGTGCGCC GAGTCTCCAA GCCCAGACAG
3301TCCTCCTTCT GCTCCCTCTG CTTTTGATGC TCTTCTCAAG GTTGCCAACT TTCTTGTACA
3361AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
3421AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
3481AGGACGAAAG TTTCTGTGCT CACCTTCCAG TACGCGTTAA GTCgacaatc aacctctgga
3541ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)