Construct: ORF TRCN0000492057
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012100.1_s317c1
- Derived from:
- ccsbBroadEn_08590
- DNA Barcode:
- AAGTTTCTGTGCTCACCTTCCAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CACNA2D3 (55799)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492057
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55799 | CACNA2D3 | calcium voltage-gated chann... | NM_018398.3 | 99.9% | 100% | 2265C>T |
| 2 | mouse | 12294 | Cacna2d3 | calcium channel, voltage-de... | NM_009785.1 | 89.9% | 98% | (many diffs) |
| 3 | mouse | 12294 | Cacna2d3 | calcium channel, voltage-de... | XM_006518486.4 | 89.5% | 97.5% | (many diffs) |
| 4 | mouse | 12294 | Cacna2d3 | calcium channel, voltage-de... | XM_011244938.3 | 87.5% | 95.3% | (many diffs) |
| 5 | mouse | 12294 | Cacna2d3 | calcium channel, voltage-de... | XM_030247616.1 | 82.1% | 89.9% | (many diffs) |
| 6 | mouse | 12294 | Cacna2d3 | calcium channel, voltage-de... | XR_001781093.2 | 46.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3342
- ORF length:
- 3273
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgggccg ggctcgccgc gccgcgcgtc ccggggggcc tcggcgcttc 121 tcgctgccgc gcttctctac gccgcgctgg gggacgtggt gcgctcggag cagcagatac 181 cgctctccgt ggtgaagctc tgggcctcgg cttttggtgg ggagataaaa tccattgctg 241 ctaagtactc cggttcccag cttctgcaaa agaaatacaa agagtatgag aaagacgttg 301 ccatagaaga aattgatggc ctccaactgg taaagaagct ggcaaagaac atggaagaga 361 tgtttcacaa gaagtctgag gccgtcaggc gtctggtgga ggctgcagaa gaagcacacc 421 tgaaacatga atttgatgca gacttacagt atgaatactt caatgctgtg ctgataaatg 481 aaagggacaa agacgggaat tttttggagc tgggaaagga attcatctta gccccaaatg 541 accattttaa taatttgcct gtgaacatca gtctaagtga cgtccaagta ccaacgaaca 601 tgtacaacaa agaccctgca attgtcaatg gggtttattg gtctgaatct ctaaacaaag 661 tttttgtaga taactttgac cgtgacccat ctctcatatg gcagtacttt ggaagtgcaa 721 agggcttttt taggcagtat ccggggatta aatgggaacc agatgagaat ggagtcattg 781 ccttcgactg caggaaccga aaatggtaca tccaggcagc aacttctccg aaagacgtgg 841 tcattttagt tgacgtcagt ggcagcatga aaggactccg tctgactatc gcgaagcaaa 901 cagtctcatc cattttggat acacttgggg atgatgactt cttcaacata attgcttata 961 atgaggagct tcactatgtg gaaccttgcc tgaatggaac tttggtgcaa gccgacagga 1021 caaacaaaga gcacttcagg gagcatctgg acaaactttt cgccaaagga attggaatgt 1081 tggatatagc tctgaatgag gccttcaaca ttctgagtga tttcaaccac acgggacaag 1141 gaagtatctg cagtcaggcc atcatgctca taactgatgg ggcggtggac acctatgata 1201 caatctttgc aaaatacaat tggccagatc gaaaggttcg catcttcaca tacctcattg 1261 gacgagaggc tgcgtttgca gacaatctaa agtggatggc ctgtgccaac aaaggatttt 1321 ttacccagat ctccaccttg gctgatgtgc aggagaatgt catggaatac cttcacgtgc 1381 ttagccggcc caaagtcatc gaccaggagc atgatgtggt gtggaccgaa gcttacattg 1441 acagcactct ccctcaggca caaaagctga ctgatgatca gggccccgtc ctgatgacca 1501 ctgtagccat gcctgtgttt agtaagcaga acgaaaccag atcgaagggc attcttctgg 1561 gagtggttgg cacagatgtc ccagtgaaag aacttctgaa gaccatcccc aaatacaagt 1621 tagggattca cggttatgcc tttgcaatca caaataatgg atatatcctg acgcatccgg 1681 aactcaggct gctgtacgaa gaaggaaaaa agcgaaggaa acctaactat agtagcgttg 1741 acctctctga ggtggagtgg gaagaccgag atgacgtgtt gagaaatgct atggtgaatc 1801 gaaagacggg gaagttttcc atggaggtga agaagacagt ggacaaaggg aaacgggttt 1861 tggtgatgac aaatgactac tattatacag acatcaaggg tactcctttc agtttaggtg 1921 tggcgctttc cagaggtcat gggaaatatt tcttccgagg gaatgtaacc atcgaagaag 1981 gcctgcatga cttagaacat cccgatgtgt ccttggcaga tgaatggtcc tactgcaaca 2041 ctgacctaca ccctgagcac cgccatctgt ctcagttaga agcgattaag ctctacctaa 2101 aaggcaaaga acctctgctc cagtgtgata aagaattgat ccaagaagtc ctttttgacg 2161 cggtggtgag tgcccccatt gaagcgtatt ggaccagcct ggccctcaac aaatctgaaa 2221 attctgacaa gggcgtggag gttgccttcc tcggcactcg cacgggcctc tccagaatca 2281 acctgtttgt cggggctgag cagctcacca atcaggactt cctgaaagct ggtgacaagg 2341 agaacatttt taacgcagac catttccctc tctggtaccg aagagccgct gagcagattc 2401 cagggagctt cgtctactcg atcccattca gcactggacc agtcaataaa agcaatgtgg 2461 tgacagcaag tacatccatc cagctcctgg atgaacggaa atctcctgtg gtggcagctg 2521 taggcattca gatgaaactt gaatttttcc aaaggaagtt ctggactgcc agcagacagt 2581 gtgcttccct ggatggcaaa tgctccatca gctgtgatga tgagactgtg aattgttacc 2641 tcatagacaa taatggattt attttggtgt ctgaagacta cacacagact ggagactttt 2701 ttggtgagat cgagggagct gtgatgaaca aattgctaac aatgggctcc tttaaaagaa 2761 ttacccttta tgactaccaa gccatgtgta gagccaacaa ggaaagcagc gatggcgccc 2821 atggcctcct ggatccttat aatgccttcc tctctgcagt aaaatggatc atgacagaac 2881 ttgtcttgtt cctggtggaa tttaacctct gcagttggtg gcactccgat atgacagcta 2941 aagcccagaa attgaaacag accctggagc cttgtgatac tgaatatcca gcattcgtct 3001 cTGAGCGCAC CATCAAGGAG ACTACAGGGA ATATTGCTTG TGAAGACTGC TCCAAGTCCT 3061 TTGTCATCCA GCAAATCCCA AGCAGCAACC TGTTCATGGT GGTGGTGGAC AGCAGCTGCC 3121 TCTGTGAATC TGTGGCCCCC ATCACCATGG CACCCATTGA AATCAGGTAT AATGAATCCC 3181 TTAAGTGTGA ACGTCTAAAG GCCCAGAAGA TCAGAAGGCG CCCAGAATCT TGTCATGGCT 3241 TCCATCCTGA GGAGAATGCA AGGGAGTGTG GGGGTGCGCC GAGTCTCCAA GCCCAGACAG 3301 TCCTCCTTCT GCTCCCTCTG CTTTTGATGC TCTTCTCAAG GTTGCCAACT TTCTTGTACA 3361 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 3421 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 3481 AGGACGAAAG TTTCTGTGCT CACCTTCCAG TACGCGTTAA GTCgacaatc aacctctgga 3541 ttacaaaatt tgtgaaagat t