Construct: ORF TRCN0000492079
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001140.1_s317c1
- Derived from:
- ccsbBroadEn_16021
- DNA Barcode:
- ACAACGCACGCCTTATCGCACCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZMIZ2 (83637)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492079
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83637 | ZMIZ2 | zinc finger MIZ-type contai... | XM_011515566.1 | 67.4% | 67.4% | 1_639del |
| 2 | human | 83637 | ZMIZ2 | zinc finger MIZ-type contai... | NM_001300959.1 | 51.2% | 51.2% | 1_1260del |
| 3 | human | 83637 | ZMIZ2 | zinc finger MIZ-type contai... | XM_005249873.2 | 49.7% | 49.7% | 1_1338del |
| 4 | human | 83637 | ZMIZ2 | zinc finger MIZ-type contai... | NM_174929.2 | 49.4% | 49.4% | 1_1356del |
| 5 | human | 83637 | ZMIZ2 | zinc finger MIZ-type contai... | XM_005249872.2 | 49.4% | 49.4% | 1_1356del |
| 6 | human | 83637 | ZMIZ2 | zinc finger MIZ-type contai... | XM_005249871.2 | 49.2% | 49.2% | 1_1365del |
| 7 | human | 83637 | ZMIZ2 | zinc finger MIZ-type contai... | XM_005249870.2 | 48.9% | 48.9% | 1_1383del |
| 8 | human | 83637 | ZMIZ2 | zinc finger MIZ-type contai... | NM_031449.4 | 48% | 48% | 1_1434del |
| 9 | human | 83637 | ZMIZ2 | zinc finger MIZ-type contai... | XM_005249869.2 | 48% | 48% | 1_1434del |
| 10 | human | 83637 | ZMIZ2 | zinc finger MIZ-type contai... | XM_005249866.2 | 47.5% | 47.5% | 1_1461del |
| 11 | human | 83637 | ZMIZ2 | zinc finger MIZ-type contai... | XM_005249867.5 | 47.5% | 47.5% | 1_1461del |
| 12 | human | 83637 | ZMIZ2 | zinc finger MIZ-type contai... | XM_005249868.4 | 47.5% | 47.5% | 1_1461del |
| 13 | human | 83637 | ZMIZ2 | zinc finger MIZ-type contai... | XM_017012674.1 | 47.5% | 47.5% | 1_1461del |
| 14 | human | 83637 | ZMIZ2 | zinc finger MIZ-type contai... | XR_926950.1 | 43.3% | 1_1608del;2415_2504del;3010_3011insTGTTTGAGAACAAC | |
| 15 | mouse | 52915 | Zmiz2 | zinc finger, MIZ-type conta... | XM_017314664.1 | 45.9% | 47.3% | (many diffs) |
| 16 | mouse | 52915 | Zmiz2 | zinc finger, MIZ-type conta... | XM_011243735.2 | 45.5% | 46.8% | (many diffs) |
| 17 | mouse | 52915 | Zmiz2 | zinc finger, MIZ-type conta... | XM_011243734.2 | 44.2% | 45.5% | (many diffs) |
| 18 | mouse | 52915 | Zmiz2 | zinc finger, MIZ-type conta... | NM_001005867.1 | 44.1% | 45.4% | (many diffs) |
| 19 | mouse | 52915 | Zmiz2 | zinc finger, MIZ-type conta... | XM_011243733.2 | 43.8% | 45.1% | (many diffs) |
| 20 | mouse | 52915 | Zmiz2 | zinc finger, MIZ-type conta... | NM_028601.3 | 42.6% | 43.9% | (many diffs) |
| 21 | mouse | 52915 | Zmiz2 | zinc finger, MIZ-type conta... | XM_006514748.2 | 42.6% | 43.9% | (many diffs) |
| 22 | mouse | 52915 | Zmiz2 | zinc finger, MIZ-type conta... | XM_006514750.3 | 42.6% | 43.9% | (many diffs) |
| 23 | mouse | 52915 | Zmiz2 | zinc finger, MIZ-type conta... | XM_011243732.1 | 42.6% | 43.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1392
- ORF length:
- 1326
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa caccaactgg ccagcctcgg tgcaggtcag cgtcaatgcc acgccgctca 121 ccatcgagcg tggcgacaac aagacctcgc acaagccact ctacctgaag catgtgtgcc 181 agccaggccg caacaccatc cagatcaccg tcaccgcctg ctgctgctcc cacctcttcg 241 tgctgcagct agtgcaccgc ccatccgtcc gctcggtgct gcagggcctc ctcaaaaagc 301 gcctcctgcc tgctgagcac tgcatcacca agataaagcg gaacttcagc agcggcacca 361 tccctggcac ccctgggccc aacggagagg acggggtgga gcagacagct atcaaggtgt 421 ccctgaagtg ccccatcacc ttccgcagga tccagctccc tgcccgaggt catgactgtc 481 gccacataca gtgctttgac ctggagtcgt acctgcagct caactgtgag cgggggactt 541 ggaggtgtcc tgtgtgcaac aagacagctt tgctggaggg cctggaggtg gaccagtaca 601 tgctgggcat cctgatttac attcagaact ctgactatga ggagatcacc atcgacccca 661 cgtgcagctg gaagccagtg cccgtgaagc ctgacatgca catcaaggag gagccggatg 721 ggccagcact gaagcgctgc cgcaccgtga gccccgccca cgtgctcatg cccagcgtga 781 tggagatgat cgccgccctg ggccccggcg ctgccccctt tgcccccctg cagcccccct 841 cagtccctgc ccccagcgac taccctggcc agggttccag cttcctgggg cctggaactt 901 tccctgagtc cttcccaccc accacgccca gcaccccaac ccttgctgag ttcaccccgg 961 gaccaccccc catctcctac cagtctgaca ttcccagcag cctcctgact tcagagaagt 1021 ctaccgcctg cctcccaagc cagatggcac cagcaggtca cctggacccc actcacaatc 1081 cTGGGACACC AGGACTACAC ACCTCCAACC TTGGGGCCCC TCCAGGTCCC CAGCTGCACC 1141 ATTCAAACCC TCCCCCAGCG TCCCGGCAGT CCTTGGGCCA AGCGAGCTTA GGACCTACGG 1201 GTGAACTGGC CTTCAGTCCT GCCACAGGCG TGATGGGGCC CCCCAGCATG TCTGGAGCCG 1261 GGGAGGCCCC AGAACCAGCT CTGGACCTGC TCCCGGAACT GACCAACCCT GATGAGCTAC 1321 TGTCCTACTT GGGCCCACCC GACCTCCCTA CGAACAACAA TGACGACCTG CTTTCTCTGT 1381 TTGAGAACAA CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1441 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1501 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACA ACGCACGCCT TATCGCACCT 1561 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t