Transcript: Human XM_005249873.2

PREDICTED: Homo sapiens zinc finger MIZ-type containing 2 (ZMIZ2), transcript variant X9, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZMIZ2 (83637)
Length:
5072
CDS:
148..2814

Additional Resources:

NCBI RefSeq record:
XM_005249873.2
NBCI Gene record:
ZMIZ2 (83637)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005249873.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000304169 CCCACGGGACTGCATTATAAG pLKO_005 1024 CDS 100% 13.200 18.480 N ZMIZ2 n/a
2 TRCN0000108198 CGGTGATGGTTCATTCGCATA pLKO.1 195 CDS 100% 4.050 5.670 N ZMIZ2 n/a
3 TRCN0000300897 CGGTGATGGTTCATTCGCATA pLKO_005 195 CDS 100% 4.050 5.670 N ZMIZ2 n/a
4 TRCN0000108196 CATCACCAAGATAAAGCGGAA pLKO.1 1743 CDS 100% 2.160 3.024 N ZMIZ2 n/a
5 TRCN0000108197 AGTCTACAAGACCCTGATAAT pLKO.1 1413 CDS 100% 13.200 10.560 N ZMIZ2 n/a
6 TRCN0000310841 GTCATGACTGTCGCCACATAC pLKO_005 1889 CDS 100% 10.800 7.560 N ZMIZ2 n/a
7 TRCN0000108199 GACCTCCCTACGAACAACAAT pLKO.1 2761 CDS 100% 5.625 3.938 N ZMIZ2 n/a
8 TRCN0000300961 GACCTCCCTACGAACAACAAT pLKO_005 2761 CDS 100% 5.625 3.938 N ZMIZ2 n/a
9 TRCN0000095243 CACCAAGATAAAGCGGAACTT pLKO.1 1746 CDS 100% 4.950 3.465 N Zmiz2 n/a
10 TRCN0000108195 CCAGCATTGATCCTTCTGTTT pLKO.1 3188 3UTR 100% 4.950 3.465 N ZMIZ2 n/a
11 TRCN0000300963 CCAGCATTGATCCTTCTGTTT pLKO_005 3188 3UTR 100% 4.950 3.465 N ZMIZ2 n/a
12 TRCN0000095242 CCACACGGTGATGGTTCATTT pLKO.1 190 CDS 100% 13.200 10.560 N Zmiz2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005249873.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12750 pDONR223 100% 97% 97% None 897_974del n/a
2 ccsbBroad304_12750 pLX_304 0% 97% 97% V5 897_974del n/a
3 TRCN0000478264 ATTATTTTCCTTCCACATTTACAT pLX_317 12.3% 97% 97% V5 897_974del n/a
4 ccsbBroadEn_16021 pDONR223 0% 49.7% 49.7% None 1_1338del n/a
5 TRCN0000492079 ACAACGCACGCCTTATCGCACCTA pLX_317 23.8% 49.7% 49.7% V5 1_1338del n/a
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