Transcript: Mouse XM_011243733.2

PREDICTED: Mus musculus zinc finger, MIZ-type containing 2 (Zmiz2), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Zmiz2 (52915)
Length:
4596
CDS:
855..3539

Additional Resources:

NCBI RefSeq record:
XM_011243733.2
NBCI Gene record:
Zmiz2 (52915)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011243733.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000095243 CACCAAGATAAAGCGGAACTT pLKO.1 2471 CDS 100% 4.950 6.930 N Zmiz2 n/a
2 TRCN0000095242 CCACACGGTGATGGTTCATTT pLKO.1 897 CDS 100% 13.200 9.240 N Zmiz2 n/a
3 TRCN0000095239 CCAGCACTGATCCTTCTGTTT pLKO.1 3892 3UTR 100% 4.950 3.465 N Zmiz2 n/a
4 TRCN0000095240 CCCTCAACTGACTTCACACAA pLKO.1 1272 CDS 100% 4.950 3.465 N Zmiz2 n/a
5 TRCN0000108196 CATCACCAAGATAAAGCGGAA pLKO.1 2468 CDS 100% 2.160 1.512 N ZMIZ2 n/a
6 TRCN0000095241 GCTCTCACTCTTCGAGAACAA pLKO.1 3515 CDS 100% 4.950 2.970 N Zmiz2 n/a
7 TRCN0000166364 CACACACACACACACACACAA pLKO.1 514 5UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011243733.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12750 pDONR223 100% 84.7% 87.3% None (many diffs) n/a
2 ccsbBroad304_12750 pLX_304 0% 84.7% 87.3% V5 (many diffs) n/a
3 TRCN0000478264 ATTATTTTCCTTCCACATTTACAT pLX_317 12.3% 84.7% 87.3% V5 (many diffs) n/a
4 ccsbBroadEn_16021 pDONR223 0% 43.8% 45.1% None (many diffs) n/a
5 TRCN0000492079 ACAACGCACGCCTTATCGCACCTA pLX_317 23.8% 43.8% 45.1% V5 (many diffs) n/a
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