Construct: ORF TRCN0000492086
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001248.1_s317c1
- Derived from:
- ccsbBroadEn_12395
- DNA Barcode:
- TAAAATCTGCCCGTAGTTAATCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SHTN1 (57698)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492086
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57698 | SHTN1 | shootin 1 | NM_001258299.1 | 100% | 100% | |
| 2 | human | 57698 | SHTN1 | shootin 1 | XM_005270002.4 | 97.3% | 97.3% | 1675_1719del |
| 3 | human | 57698 | SHTN1 | shootin 1 | XM_006717931.4 | 90.3% | 90.4% | 1674_1851delinsA |
| 4 | human | 57698 | SHTN1 | shootin 1 | NM_001258300.1 | 89.2% | 89.2% | 0_1ins180 |
| 5 | human | 57698 | SHTN1 | shootin 1 | NM_001127211.3 | 88.3% | 88.4% | 1674_1893delinsA |
| 6 | human | 57698 | SHTN1 | shootin 1 | NM_018330.7 | 81.6% | 81.1% | (many diffs) |
| 7 | human | 57698 | SHTN1 | shootin 1 | NM_001258298.1 | 78.8% | 78.9% | 0_1ins180;1494_1713delinsA |
| 8 | mouse | 71653 | Shtn1 | shootin 1 | NM_001114312.1 | 78.4% | 82.8% | (many diffs) |
| 9 | mouse | 71653 | Shtn1 | shootin 1 | NM_175172.4 | 71.5% | 76.3% | (many diffs) |
| 10 | mouse | 71653 | Shtn1 | shootin 1 | XR_386512.3 | 35.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1740
- ORF length:
- 1674
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cagctcggac gaagagaagc agctgcagct cattaccagt ctgaaggagc 121 aagcaatagg cgaatatgaa gaccttagag cagagaacca gaaaacaaag gagaagtgtg 181 acaaaattag gcaagaacga gatgaagccg ttaaaaaact ggaagaattt cagaaaattt 241 ctcacatggt catagaggaa gttaatttca tgcagaacca tcttgaaata gagaagactt 301 gtcgagaaag tgctgaagct ttggcaacaa agctaaataa agaaaataaa acgttgaaaa 361 gaatcagcat gttgtacatg gccaagctgg gaccagatgt aataactgaa gagataaaca 421 ttgatgatga agattcgact acagacacag acggtgccgc cgagacttgt gtctcagtac 481 agtgtcagaa gcaaattaaa gaacttcgag atcaaattgt atctgttcag gaggaaaaga 541 agattttagc cattgagctg gaaaatctca agagcaaact cgtagaagta attgaagaag 601 taaataaagt taaacaagaa aagactgttt taaattcaga agttcttgaa cagagaaaag 661 tcttagaaaa atgcaataga gtgtccatgt tagctgtaga agagtatgag gagatgcaag 721 taaacctgga gctggagaag gaccttcgaa agaaagcaga gtcatttgca caagagatgt 781 tcattgagca aaacaagcta aagagacaaa gccaccttct gctgcagagc tccatccctg 841 atcagcagct tttgaaagct ttagacgaaa atgcaaaact cacccagcaa cttgaagaag 901 agagaattca gcatcaacaa aaggtcaaag aattagaaga gcaactagaa aatgaaacac 961 tccacaaaga aatacacaac ctcaaacagc aactggagct tctagaggaa gataaaaagg 1021 aattggaatt gaaatatcag aattctgaag agaaagccag aaatttaaag cactctgttg 1081 atgaactcca gaaacgagtg aaccagtctg agaattcagt acctccacca cctcctcctc 1141 caccaccact tccccctcca cctcccaatc ctatccgatc cctcatgtcc atgatccgga 1201 aacgatccca ccccagtggc agtggtgcta agaaagaaaa ggcaactcaa ccagaaacaa 1261 ctgaagaagt cacagatcta aagaggcaag cagttGAAGA GATGATGGAT AGAATTAAAA 1321 AGGGAGTTCA TCTTAGACCC GTTAATCAGA CAGCCAGACC GAAGACAAAG CCAGAATCTT 1381 CGAAAGGCTG CGAAAGTGCA GTGGATGAAC TAAAAGGAAT ACTGGGGACA CTTAACAAAT 1441 CCACTAGTTC AAGAAGCTTA AAATCCCTTG ACCCTGAAAA CAGTGAAACT GAGTTAGAAA 1501 GGATTTTGCG TCGCAGAAAG GTGACAGCAG AAGCAGATAG CAGTAGTCCA ACTGGGATAT 1561 TAGCCACCTC AGAGTCCAAA TCCATGCCAG TGTTGGGTTC TGTATCCAGT GTAACAAAAA 1621 CAGCCTTGAA CAAGAAAACT CTGGAGGCAG AATTCAACAG CCCGTCCCCC CCAACACCTG 1681 AGCCAGGTGA AGGGCCCCGT AAATTGGAAG GATGCACAAG TTCCAAGGTT ACGTTTCAAT 1741 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1801 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1861 TTTATATATC TTGTGGAAAG GACGATAAAA TCTGCCCGTA GTTAATCAGA CGCGTTAAGT 1921 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt