Construct: ORF TRCN0000492100

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019384.2_s317c1
DNA Barcode:
CGAACTACGAGCACTCCTTCAATT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKCI (5584)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492100
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5584 PRKCI protein kinase C iota NM_002740.6 98.4% 98.3% 1_27del;1788_1789insG
2 human 5584 PRKCI protein kinase C iota XM_017006860.1 79% 75.7% 0_1ins283;54_55ins86;1392_1393insG
3 human 5584 PRKCI protein kinase C iota XR_001740206.1 34.7% 1_192del;1367_1368ins88;1866_4722delinsG
4 mouse 18759 Prkci protein kinase C, iota NM_008857.3 88.9% 96.6% (many diffs)
5 mouse 18759 Prkci protein kinase C, iota XM_006535411.3 84.5% 91.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1830
ORF length:
1764
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaat
61tcaccatgtc ccacacggtc gcaggcggcg gcagcgggga ccattcccac caggtccggg
121tgaaagccta ctaccgcggg gatatcatga taacacattt tgaaccttcc atctcctttg
181agggcctttg caatgaggtt cgagacatgt gttcttttga caacgaacag ctcttcacca
241tgaaatggat agatgaggaa ggagacccgt gtacagtatc atctcagttg gagttagaag
301aagcctttag actttatgag ctaaacaagg attctgaact cttgattcat gtgttccctt
361gtgtaccaga acgtcctggg atgccttgtc caggagaaga taaatccatc taccgtagag
421gtgcacgccg ctggagaaag ctttattgtg ccaatggcca cactttccaa gccaagcgtt
481tcaacaggcg tgctcactgt gccatctgca cagaccgaat atggggactt ggacgccaag
541gatataagtg catcaactgc aaactcttgg ttcataagaa gtgccataaa ctcgtcacaa
601ttgaatgtgg gcggcattct ttgccacagg aaccagtgat gcccatggat cagtcatcca
661tgcattctga ccatgcacag acagtaattc catataatcc ttcaagtcat gagagtttgg
721atcaagttgg tgaagaaaaa gaggcaatga acaccaggga aagtggcaaa gcttcatcca
781gtctaggtct tcaggatttt gatttgctcc gggtaatagg aagaggaagt tatgccaaag
841tactgttggt tcgattaaaa aaaacagatc gtatttatgc aatgaaagtt gtgaaaaaag
901agcttgttaa tgatgatgag gatattgatt gggtacagac agagaagcat gtgtttgagc
961aggcatccaa tcatcctttc cttgttgggc tgcattcttg ctttcagaca gaaagcagat
1021tgttctttgt tatagagtat gtaaatggag gagacctaat gtttcatatg cagcgacaaa
1081gaaaacttcc tgaagaacat gccagatttt actctgcaga aatcagtcta gcattaaatt
1141atcttcatga gcgagggata atttatagag atttgaaact ggacaatgta ttactggact
1201ctgaaggcca cattaaactc actgactacg gcatgtgtaa ggaaggatta cggccaggag
1261atacaaccag cactttctgt ggtactccta attacattgc tcctgaaatt ttaagaggag
1321aagattatgg tttcagtgtt gactggtggg ctcttggagt gctcatgttt gagatgatgg
1381caggaaggtc tccatttgat attgttggga gcTCCGATAA CCCTGACCAG AACACAGAGG
1441ATTATCTCTT CCAAGTTATT TTGGAAAAAC AAATTCGCAT ACCACGTTCT CTGTCTGTAA
1501AAGCTGCAAG TGTTCTGAAG AGTTTTCTTA ATAAGGACCC TAAGGAACGA TTGGGTTGTC
1561ATCCTCAAAC AGGATTTGCT GATATTCAGG GACACCCGTT CTTCCGAAAT GTTGATTGGG
1621ATATGATGGA GCAAAAACAG GTGGTACCTC CCTTTAAACC AAATATTTCT GGGGAATTTG
1681GTTTGGACAA CTTTGATTCT CAGTTTACTA ATGAACCTGT CCAGCTCACT CCAGATGACG
1741ATGACATTGT GAGGAAGATT GATCAGTCTG AATTTGAAGG TTTTGAGTAT ATCAATCCTC
1801TTTTGATGTC TGCAGAAGAA TGTGTCGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
1861GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1921AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGAACTAC
1981GAGCACTCCT TCAATTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2041aagatt

Download FASTA (ORF) (Full)

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