Construct: ORF TRCN0000492100
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019384.2_s317c1
- DNA Barcode:
- CGAACTACGAGCACTCCTTCAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKCI (5584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492100
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5584 | PRKCI | protein kinase C iota | NM_002740.6 | 98.4% | 98.3% | 1_27del;1788_1789insG |
| 2 | human | 5584 | PRKCI | protein kinase C iota | XM_017006860.1 | 79% | 75.7% | 0_1ins283;54_55ins86;1392_1393insG |
| 3 | human | 5584 | PRKCI | protein kinase C iota | XR_001740206.1 | 34.7% | 1_192del;1367_1368ins88;1866_4722delinsG | |
| 4 | mouse | 18759 | Prkci | protein kinase C, iota | NM_008857.3 | 88.9% | 96.6% | (many diffs) |
| 5 | mouse | 18759 | Prkci | protein kinase C, iota | XM_006535411.3 | 84.5% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1830
- ORF length:
- 1764
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaat 61 tcaccatgtc ccacacggtc gcaggcggcg gcagcgggga ccattcccac caggtccggg 121 tgaaagccta ctaccgcggg gatatcatga taacacattt tgaaccttcc atctcctttg 181 agggcctttg caatgaggtt cgagacatgt gttcttttga caacgaacag ctcttcacca 241 tgaaatggat agatgaggaa ggagacccgt gtacagtatc atctcagttg gagttagaag 301 aagcctttag actttatgag ctaaacaagg attctgaact cttgattcat gtgttccctt 361 gtgtaccaga acgtcctggg atgccttgtc caggagaaga taaatccatc taccgtagag 421 gtgcacgccg ctggagaaag ctttattgtg ccaatggcca cactttccaa gccaagcgtt 481 tcaacaggcg tgctcactgt gccatctgca cagaccgaat atggggactt ggacgccaag 541 gatataagtg catcaactgc aaactcttgg ttcataagaa gtgccataaa ctcgtcacaa 601 ttgaatgtgg gcggcattct ttgccacagg aaccagtgat gcccatggat cagtcatcca 661 tgcattctga ccatgcacag acagtaattc catataatcc ttcaagtcat gagagtttgg 721 atcaagttgg tgaagaaaaa gaggcaatga acaccaggga aagtggcaaa gcttcatcca 781 gtctaggtct tcaggatttt gatttgctcc gggtaatagg aagaggaagt tatgccaaag 841 tactgttggt tcgattaaaa aaaacagatc gtatttatgc aatgaaagtt gtgaaaaaag 901 agcttgttaa tgatgatgag gatattgatt gggtacagac agagaagcat gtgtttgagc 961 aggcatccaa tcatcctttc cttgttgggc tgcattcttg ctttcagaca gaaagcagat 1021 tgttctttgt tatagagtat gtaaatggag gagacctaat gtttcatatg cagcgacaaa 1081 gaaaacttcc tgaagaacat gccagatttt actctgcaga aatcagtcta gcattaaatt 1141 atcttcatga gcgagggata atttatagag atttgaaact ggacaatgta ttactggact 1201 ctgaaggcca cattaaactc actgactacg gcatgtgtaa ggaaggatta cggccaggag 1261 atacaaccag cactttctgt ggtactccta attacattgc tcctgaaatt ttaagaggag 1321 aagattatgg tttcagtgtt gactggtggg ctcttggagt gctcatgttt gagatgatgg 1381 caggaaggtc tccatttgat attgttggga gcTCCGATAA CCCTGACCAG AACACAGAGG 1441 ATTATCTCTT CCAAGTTATT TTGGAAAAAC AAATTCGCAT ACCACGTTCT CTGTCTGTAA 1501 AAGCTGCAAG TGTTCTGAAG AGTTTTCTTA ATAAGGACCC TAAGGAACGA TTGGGTTGTC 1561 ATCCTCAAAC AGGATTTGCT GATATTCAGG GACACCCGTT CTTCCGAAAT GTTGATTGGG 1621 ATATGATGGA GCAAAAACAG GTGGTACCTC CCTTTAAACC AAATATTTCT GGGGAATTTG 1681 GTTTGGACAA CTTTGATTCT CAGTTTACTA ATGAACCTGT CCAGCTCACT CCAGATGACG 1741 ATGACATTGT GAGGAAGATT GATCAGTCTG AATTTGAAGG TTTTGAGTAT ATCAATCCTC 1801 TTTTGATGTC TGCAGAAGAA TGTGTCGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1861 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1921 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGAACTAC 1981 GAGCACTCCT TCAATTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2041 aagatt