Construct: ORF TRCN0000492135
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004326.1_s317c1
- Derived from:
- ccsbBroadEn_00593
- DNA Barcode:
- TCCAGCTCACAGAAAAGCGCCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NR5A2 (2494)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492135
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2494 | NR5A2 | nuclear receptor subfamily ... | NM_003822.5 | 100% | 100% | |
| 2 | human | 2494 | NR5A2 | nuclear receptor subfamily ... | XM_011509380.1 | 95% | 94% | (many diffs) |
| 3 | human | 2494 | NR5A2 | nuclear receptor subfamily ... | XM_011509381.3 | 95% | 94% | (many diffs) |
| 4 | human | 2494 | NR5A2 | nuclear receptor subfamily ... | XM_011509384.2 | 95% | 94% | (many diffs) |
| 5 | human | 2494 | NR5A2 | nuclear receptor subfamily ... | XM_017000904.1 | 95% | 94% | (many diffs) |
| 6 | human | 2494 | NR5A2 | nuclear receptor subfamily ... | NM_001276464.1 | 94.7% | 94.7% | 0_1ins78 |
| 7 | human | 2494 | NR5A2 | nuclear receptor subfamily ... | XM_005245062.3 | 94.7% | 94.7% | 0_1ins78 |
| 8 | human | 2494 | NR5A2 | nuclear receptor subfamily ... | XM_011509382.1 | 94.7% | 94.7% | 0_1ins78 |
| 9 | human | 2494 | NR5A2 | nuclear receptor subfamily ... | NM_205860.3 | 91.4% | 91.4% | 64_201del |
| 10 | mouse | 26424 | Nr5a2 | nuclear receptor subfamily ... | NM_001159769.2 | 82.7% | 85.6% | (many diffs) |
| 11 | mouse | 26424 | Nr5a2 | nuclear receptor subfamily ... | XM_006529622.4 | 82.7% | 85.6% | (many diffs) |
| 12 | mouse | 26424 | Nr5a2 | nuclear receptor subfamily ... | XM_006529621.3 | 78.8% | 82.4% | (many diffs) |
| 13 | mouse | 26424 | Nr5a2 | nuclear receptor subfamily ... | NM_030676.3 | 75.9% | 79.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1554
- ORF length:
- 1485
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcttctaat tcagatactg gggatttaca agagtcttta aagcacggac 121 ttacacctat tgtgtctcaa tttaaaatgg tgaattactc ctatgatgaa gatctggaag 181 agctttgtcc cgtgtgtgga gataaagtgt ctgggtacca ttatgggctc ctcacctgtg 241 aaagctgcaa gggatttttt aagcgaacag tccaaaataa taaaaggtac acatgtatag 301 aaaaccagaa ctgccaaatt gacaaaacac agagaaagcg ttgtccttac tgtcgttttc 361 aaaaatgtct aagtgttgga atgaagctag aagctgtaag ggccgaccga atgcgtggag 421 gaaggaataa gtttgggcca atgtacaaga gagacagggc cctgaagcaa cagaaaaaag 481 ccctcatccg agccaatgga cttaagctag aagccatgtc tcaggtgatc caagctatgc 541 cctctgacct gaccatttcc tctgcaattc aaaacatcca ctctgcctcc aaaggcctac 601 ctctgaacca tgctgccttg cctcctacag actatgacag aagtcccttt gtaacatccc 661 ccattagcat gacaatgccc cctcacggca gcctgcaagg ttaccaaaca tatggccact 721 ttcctagccg ggccatcaag tctgagtacc cagaccccta taccagctca cccgagtcca 781 taatgggcta ttcatatatg gatagttacc agacgagctc tccagcaagc atcccacatc 841 tgatactgga acttttgaag tgtgagccag atgagcctca agtccaggct aaaatcatgg 901 cctatttgca gcaagagcag gctaaccgaa gcaagcacga aaagctgagc acctttgggc 961 ttatgtgcaa aatggcagat caaactctct tctccattgt cgagtgggcc aggagtagta 1021 tcttcttcag agaacttaag gttgatgacc aaatgaagct gcttcagaac tgctggagtg 1081 agctcttaat cctcgaccac atttaccgac aagtggtaca tggaaaggaa ggatccaTCT 1141 TCCTGGTTAC TGGGCAACAA GTGGACTATT CCATAATAGC ATCACAAGCC GGAGCCACCC 1201 TCAACAACCT CATGAGTCAT GCACAGGAGT TAGTGGCAAA ACTTCGTTCT CTCCAGTTTG 1261 ATCAACGAGA GTTCGTATGT CTGAAATTCT TGGTGCTCTT TAGTTTAGAT GTCAAAAACC 1321 TTGAAAACTT CCAGCTGGTA GAAGGTGTCC AGGAACAAGT CAATGCCGCC CTGCTGGACT 1381 ACACAATGTG TAACTACCCG CAGCAGACAG AGAAATTTGG ACAGCTACTT CTTCGACTAC 1441 CCGAAATCCG GGCCATCAGT ATGCAGGCTG AAGAATACCT CTACTACAAG CACCTGAACG 1501 GGGATGTGCC CTATAATAAC CTTCTCATTG AAATGTTGCA TGCCAAAAGA GCATTGCCAA 1561 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1621 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1681 TATCTTGTGG AAAGGACGAT CCAGCTCACA GAAAAGCGCC CCCACGCGTT AAGTCgacaa 1741 tcaacctctg gattacaaaa tttgtgaaag att