Construct: ORF TRCN0000492149
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000137.1_s317c1
- Derived from:
- ccsbBroadEn_05886
- DNA Barcode:
- TTAGCTCCGTGCCGCGATATGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BDNF (627)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492149
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 627 | BDNF | brain derived neurotrophic ... | NM_001143805.1 | 99.7% | 99.5% | 196G>A;354T>C |
| 2 | human | 627 | BDNF | brain derived neurotrophic ... | NM_001143806.1 | 99.7% | 99.5% | 196G>A;354T>C |
| 3 | human | 627 | BDNF | brain derived neurotrophic ... | NM_001143807.2 | 99.7% | 99.5% | 196G>A;354T>C |
| 4 | human | 627 | BDNF | brain derived neurotrophic ... | NM_001143808.1 | 99.7% | 99.5% | 196G>A;354T>C |
| 5 | human | 627 | BDNF | brain derived neurotrophic ... | NM_001143811.1 | 99.7% | 99.5% | 196G>A;354T>C |
| 6 | human | 627 | BDNF | brain derived neurotrophic ... | NM_001143812.1 | 99.7% | 99.5% | 196G>A;354T>C |
| 7 | human | 627 | BDNF | brain derived neurotrophic ... | NM_001143813.2 | 99.7% | 99.5% | 196G>A;354T>C |
| 8 | human | 627 | BDNF | brain derived neurotrophic ... | NM_001143814.2 | 99.7% | 99.5% | 196G>A;354T>C |
| 9 | human | 627 | BDNF | brain derived neurotrophic ... | NM_001143816.1 | 99.7% | 99.5% | 196G>A;354T>C |
| 10 | human | 627 | BDNF | brain derived neurotrophic ... | NM_001709.5 | 99.7% | 99.5% | 196G>A;354T>C |
| 11 | human | 627 | BDNF | brain derived neurotrophic ... | NM_170732.4 | 99.7% | 99.5% | 196G>A;354T>C |
| 12 | human | 627 | BDNF | brain derived neurotrophic ... | NM_170733.3 | 99.7% | 99.5% | 196G>A;354T>C |
| 13 | human | 627 | BDNF | brain derived neurotrophic ... | NM_170735.5 | 99.7% | 99.5% | 196G>A;354T>C |
| 14 | human | 627 | BDNF | brain derived neurotrophic ... | NM_170731.5 | 96.6% | 96.4% | 1_24del;220G>A;378T>C |
| 15 | human | 627 | BDNF | brain derived neurotrophic ... | NM_170734.3 | 94% | 93.8% | 1_45del;241G>A;399T>C |
| 16 | human | 627 | BDNF | brain derived neurotrophic ... | NM_001143809.1 | 89.2% | 89.1% | 1_87del;283G>A;441T>C |
| 17 | human | 627 | BDNF | brain derived neurotrophic ... | NM_001143810.1 | 74.8% | 74.7% | 1_246del;442G>A;600T>C |
| 18 | human | 627 | BDNF | brain derived neurotrophic ... | XM_011520280.2 | 74.8% | 74.7% | 1_246del;442G>A;600T>C |
| 19 | mouse | 12064 | Bdnf | brain derived neurotrophic ... | NM_001048139.1 | 91.1% | 96.3% | (many diffs) |
| 20 | mouse | 12064 | Bdnf | brain derived neurotrophic ... | NM_001048141.1 | 91.1% | 96.3% | (many diffs) |
| 21 | mouse | 12064 | Bdnf | brain derived neurotrophic ... | NM_001048142.1 | 91.1% | 96.3% | (many diffs) |
| 22 | mouse | 12064 | Bdnf | brain derived neurotrophic ... | NM_001285416.1 | 91.1% | 96.3% | (many diffs) |
| 23 | mouse | 12064 | Bdnf | brain derived neurotrophic ... | NM_001285417.1 | 91.1% | 96.3% | (many diffs) |
| 24 | mouse | 12064 | Bdnf | brain derived neurotrophic ... | NM_001285418.1 | 91.1% | 96.3% | (many diffs) |
| 25 | mouse | 12064 | Bdnf | brain derived neurotrophic ... | NM_001285419.1 | 91.1% | 96.3% | (many diffs) |
| 26 | mouse | 12064 | Bdnf | brain derived neurotrophic ... | NM_001285420.1 | 91.1% | 96.3% | (many diffs) |
| 27 | mouse | 12064 | Bdnf | brain derived neurotrophic ... | NM_001285421.1 | 91.1% | 96.3% | (many diffs) |
| 28 | mouse | 12064 | Bdnf | brain derived neurotrophic ... | NM_001285422.1 | 91.1% | 96.3% | (many diffs) |
| 29 | mouse | 12064 | Bdnf | brain derived neurotrophic ... | NM_001316310.1 | 91.1% | 96.3% | (many diffs) |
| 30 | mouse | 12064 | Bdnf | brain derived neurotrophic ... | NM_007540.4 | 88.3% | 93.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 807
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac catccttttc cttactatgg ttatttcata ctttggttgc atgaaggctg 121 cccccatgaa agaagcaaac atccgaggac aaggtggctt ggcctaccca ggtgtgcgga 181 cccatgggac tctggagagc gtgaatgggc ccaaggcagg ttcaagaggc ttgacatcat 241 tggctgacac tttcgaacac atgatagaag agctgttgga tgaggaccag aaagttcggc 301 ccaatgaaga aaacaataag gacgcagact tgtacacgtc cagggtgatg ctcagtagtc 361 aagtgccttt ggagcctcct cttctctttc tgctGGAGGA ATACAAAAAT TACCTAGACG 421 CTGCAAACAT GTCCATGAGG GTCCGGCGCC ACTCTGACCC TGCCCGCCGA GGGGAGCTGA 481 GCGTGTGTGA CAGTATTAGT GAGTGGGTAA CGGCGGCAGA CAAAAAGACT GCAGTGGACA 541 TGTCGGGCGG GACGGTCACA GTCCTTGAAA AGGTCCCTGT ATCAAAAGGC CAACTGAAGC 601 AATACTTCTA CGAGACCAAG TGCAATCCCA TGGGTTACAC AAAAGAAGGC TGCAGGGGCA 661 TAGACAAAAG GCATTGGAAC TCCCAGTGCC GAACTACCCA GTCGTACGTG CGGGCCCTTA 721 CCATGGATAG CAAAAAGAGA ATTGGCTGGC GATTCATAAG GATAGACACT TCTTGTGTAT 781 GTACATTGAC CATTAAAAGG GGAAGATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 841 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 901 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTAGCTCC 961 GTGCCGCGAT ATGAAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1021 aagatt