Construct: ORF TRCN0000492175
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020273.2_s317c1
- DNA Barcode:
- CACTGTTGATGCCTACAGTTGAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K6 (5608)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492175
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5608 | MAP2K6 | mitogen-activated protein k... | NM_002758.4 | 99.7% | 99.7% | 1002_1003insTTG |
| 2 | human | 5608 | MAP2K6 | mitogen-activated protein k... | XM_011525026.2 | 97.9% | 97.3% | (many diffs) |
| 3 | human | 5608 | MAP2K6 | mitogen-activated protein k... | NM_001330450.2 | 82.9% | 82.9% | 0_1ins168;834_835insTTG |
| 4 | human | 5608 | MAP2K6 | mitogen-activated protein k... | XM_005257516.2 | 82.9% | 82.9% | 0_1ins168;834_835insTTG |
| 5 | human | 5608 | MAP2K6 | mitogen-activated protein k... | XM_006721975.3 | 82.9% | 82.9% | 0_1ins168;834_835insTTG |
| 6 | human | 5608 | MAP2K6 | mitogen-activated protein k... | XM_011525027.3 | 82.9% | 82.9% | 0_1ins168;834_835insTTG |
| 7 | mouse | 26399 | Map2k6 | mitogen-activated protein k... | NM_011943.2 | 90.7% | 97.6% | (many diffs) |
| 8 | mouse | 26399 | Map2k6 | mitogen-activated protein k... | XM_011249034.2 | 81% | 85.8% | (many diffs) |
| 9 | mouse | 26399 | Map2k6 | mitogen-activated protein k... | XM_006533377.3 | 71.3% | 77.3% | (many diffs) |
| 10 | mouse | 26399 | Map2k6 | mitogen-activated protein k... | XM_006533378.3 | 71.3% | 77.3% | (many diffs) |
| 11 | mouse | 26399 | Map2k6 | mitogen-activated protein k... | XM_011249035.2 | 71.3% | 77.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1077
- ORF length:
- 1005
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtctcag tcgaaaggca agaagcgaaa ccctggcctt aaaattccaa 121 aagaagcatt tgaacaacct cagaccagtt ccacaccacc tcgagattta gactccaagg 181 cttgcatttc tattggaaat cagaactttg aggtgaaggc agatgacctg gagcctataa 241 tggaactggg acgaggtgcg tacggggtgg tggagaagat gcggcacgtg cccagcgggc 301 agatcatggc agtgaagcgg atccgagcca cagtaaatag ccaggaacag aaacggctac 361 tgatggattt ggatatttcc atgaggacgg tggactgtcc attcactgtc accttttatg 421 gcgcactgtt tcgggagggt gatgtgtgga tctgcatgga gctcatggat acatcactag 481 ataaattcta caaacaagtt attgataaag gccagacaat tccagaggac atcttaggga 541 aaatagcagt ttctattgta aaagcattag aacatttaca tagtaagctg tctgtcattc 601 acagagacgt caagccttct aatgtactca tcaatgctct cggtcaagtg aagatgtgcg 661 attttggaat cagtggctac ttggtggact ctgttgctaa aacaattgat gcaggttgca 721 aaccatacat ggccccTGAA AGAATAAACC CAGAGCTCAA CCAGAAGGGA TACAGTGTGA 781 AGTCTGACAT TTGGAGTCTG GGCATCACGA TGATTGAGTT GGCCATCCTT CGATTTCCCT 841 ATGATTCATG GGGAACTCCA TTTCAGCAGC TCAAACAGGT GGTAGAGGAG CCATCGCCAC 901 AACTCCCAGC AGACAAGTTC TCTGCAGAGT TTGTTGACTT TACCTCACAG TGCTTAAAGA 961 AGAATTCCAA AGAACGGCCT ACATACCCAG AGCTAATGCA ACATCCATTT TTCACCCTAC 1021 ATGAATCCAA AGGAACAGAT GTGGCATCTT TTGTAAAACT GATTCTTGGA GACTTGTGAG 1081 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1141 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1201 TTTATATATC TTGTGGAAAG GACGACACTG TTGATGCCTA CAGTTGAGTA CGCGTTAAGT 1261 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt