Construct: ORF TRCN0000492187

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016041.1_s317c1
Derived from:
ccsbBroadEn_09360
DNA Barcode:
TCGTGGGCATTCTTTATCGAAATA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MYOCD (93649)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492187
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 93649 MYOCD myocardin NM_001146312.2 99.9% 100% 936A>G
2 human 93649 MYOCD myocardin XM_017025342.1 95.7% 95.7% 589_590ins126;810A>G
3 human 93649 MYOCD myocardin XM_005256863.1 95.6% 95.6% 936A>G;2200_2201ins129
4 human 93649 MYOCD myocardin NM_153604.3 95% 95.1% 936A>G;2056_2057ins144
5 human 93649 MYOCD myocardin XM_005256864.1 91.9% 91.9% 0_1ins237;699A>G
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3027
ORF length:
2958
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttgtcaacat gacactcctg gggtctgagc attccttgct gattaggagc aagttcagat
121cagttttaca gttaagactt caacaaagaa ggacccagga acaactggct aaccaaggca
181taataccacc actgaaacgt ccagctgaat tccatgagca aagaaaacat ttggatagtg
241acaaggctaa aaattccctg aagcgcaaag ccagaaacag gtgcaacagt gccgacttgg
301ttaatatgca catactccaa gcttccactg cagagaggtc cattccaact gctcagatga
361agctgaaaag agcccgactc gccgatgatc tcaatgaaaa aattgctcta cgaccagggc
421cactggagct ggtggaaaaa aacattcttc ctgtggattc tgctgtgaaa gaggccataa
481aaggtaacca ggtgagtttc tccaaatcca cggatgcttt tgcctttgaa gaggacagca
541gcagcgatgg gctttctccg gatcagactc gaagtgaaga cccccaaaac tcagcgggat
601ccccgccaga cgctaaagcc tcagataccc cttcgacagg ttctctgggg acaaaccagg
661atcttgcttc tggctcagaa aatgacagaa atgactcagc ctcacagccc agccaccagt
721cagatgcggg gaagcagggg cttggccccc ccagcacccc catagccgtg catgctgctg
781taaagtccaa atccttgggt gacagtaaga accgccacaa aaagcccaag gaccccaagc
841caaaggtgaa gaagcttaaa tatcaccagt acattccccc agaccagaag gcagagaagt
901cccctccacc tatggactca gcctacgctc ggctgctcca gcaacagcag ctgttcctgc
961agctccaaat cctcagccag cagcagcagc agcagcaaca ccggttcagc tacctaggga
1021tgcaccaagc tcagcttaag gaaccaaatg aacagatggt cagaaatcca aactcttctt
1081caacgccact gagcaatacc cccttgtctc ctgtcaaaaa cagtttttct ggacaaactg
1141gtgtctcttc tttcaaacca ggcccactcc cacctaacct ggatgatctg aaggtctctg
1201aattaagaca acagcttcga attcggggct tgcctgtgtc aggcaccaaa acggctctca
1261tggaccggct tcgacccttc caggactgct ctggcaaccc agtgccgaac tttggggata
1321taacgactgt cacttttcct gtcacaccca acacgctgcc caattaccag tcttcctctt
1381ctaccagtgc cctgtccaac ggcttctacc actttggcag caccagctcc agccccccga
1441tctccccagc ctcctctgac ctgtcagtcg ctgggtccct gccggacacc ttcaatgatg
1501cctccccctc cttcggcctg cacccgtccc cagtccacgt gtgcacggag gaaagtctca
1561tgagcagcct gaatgggggc tctgttcctt ctgagctgga tgggctggac tccgagaagg
1621acaagatgct ggtggagaag cagaaggtga tcaatgaact cacctggaaa ctccagcaag
1681agcagaggca ggtggaggag ctgaggatgc agcttcagaa gcagaaaagg aataactgtt
1741cagagaagaa gccgctgcct ttcctggctg cctccatcaa gcaggaagag gctgtctcca
1801gctgtccttt tgcatcccaa gtacctgtga aaagacaaag cagcagctca gagtgtcacc
1861caccggcttg tgaagctgct caactccagc ctcttggaaa tgctcattgt gtggagtcct
1921cagatcaaac caatgtactt tcttccacat ttctcagccc ccagtgttcc cctcagcatt
1981caccgctggg ggctgtgaaa agcccacagc acatcagttt gcccccatca cccaacaacc
2041ctcactttct gccctcatcc tccggggccc agggagaagg gcacagggtc tcctcgccca
2101tcagcagcca ggtgtgcact gcacagaact caggagcaca cgatggccat cctccaagct
2161tctctcccca ttcttccagc ctccacccgc ccttctctgg agcccaagca gacagcagtc
2221atggtgccgg gggaaaccct tgtcccaaaa gcccatgtgt acagcaaaag atggctggtt
2281tacactcttc tgataaggtg gggccaaagt tttcaattcc atccccaact ttttctaagt
2341caagttcagc aatttcagag gtaacacagc ctccatccta tgaagatgcc gtaaagcagc
2401aaatgacccg gagtcagcag atggatgaac tcctggacgt gcttattgaa agcggagaaa
2461tgccagcaga cgctagagag gatcactcat gtcttcaaaa agtcccaaag atacccagat
2521cttcccgaag tccaactgct gtcctcacca agccctcggc ttcctttgaa caagcctctt
2581caggcagcca gaTCCCCTTT GATCCCTATG CCACCGACAG TGATGAGCAT CTTGAAGTCT
2641TATTAAATTC CCAGAGCCCC CTAGGAAAGA TGAGTGATGT CACCCTTCTA AAAATTGGGA
2701GCGAAGAGCC TCACTTTGAT GGGATAATGG ATGGATTCTC TGGGAAGGCT GCAGAAGACC
2761TCTTCAATGC ACATGAGATC TTGCCAGGCC CCCTCTCTCC AATGCAGACA CAGTTTTCAC
2821CCTCTTCTGT GGACAGCAAT GGGCTGCAGT TAAGCTTCAC TGAATCTCCC TGGGAAACCA
2881TGGAGTGGCT GGACCTCACT CCGCCAAATT CCACACCAGG CTTTAGCGCC CTCACCACCA
2941GCAGCCCCAG CATCTTCAAC ATCGATTTCC TGGATGTCAC TGATCTCAAT TTGAATTCTT
3001CCATGGACCT TCACTTGCAG CAGTGGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG
3061GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
3121AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCGTGGGC
3181ATTCTTTATC GAAATAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
3241aagatt

Download FASTA (ORF) (Full)