Construct: ORF TRCN0000492212
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020424.2_s317c1
- DNA Barcode:
- TGCCAACATGACGCCTACAAGGAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MET (4233)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492212
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4233 | MET | MET proto-oncogene, recepto... | NM_000245.4 | 99.9% | 100% | 2121T>C;2124A>G |
| 2 | human | 4233 | MET | MET proto-oncogene, recepto... | NM_001127500.3 | 98.6% | 98.7% | 2121T>C;2124A>G;2263_2316del |
| 3 | human | 4233 | MET | MET proto-oncogene, recepto... | XM_011516223.1 | 98.6% | 98.6% | 1_57del;2178T>C;2181A>G |
| 4 | human | 4233 | MET | MET proto-oncogene, recepto... | NM_001324402.2 | 69% | 69% | 0_1ins1290;831T>C;834A>G |
| 5 | human | 4233 | MET | MET proto-oncogene, recepto... | XM_006715990.2 | 69% | 69% | 0_1ins1290;831T>C;834A>G |
| 6 | human | 4233 | MET | MET proto-oncogene, recepto... | NM_001324401.2 | 66.6% | 65.9% | (many diffs) |
| 7 | human | 4233 | MET | MET proto-oncogene, recepto... | XR_001744772.1 | 61% | (many diffs) | |
| 8 | mouse | 17295 | Met | met proto-oncogene | NM_008591.2 | 85.7% | 88.9% | (many diffs) |
| 9 | mouse | 17295 | Met | met proto-oncogene | XM_011241042.2 | 85.7% | 88.9% | (many diffs) |
| 10 | mouse | 17295 | Met | met proto-oncogene | XM_011241043.2 | 85.7% | 88.9% | (many diffs) |
| 11 | mouse | 17295 | Met | met proto-oncogene | XM_006505002.3 | 82.4% | 85.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 4254
- ORF length:
- 4170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatgaagg cccccgctgt gcttgcacct ggcatcctcg 121 tgctcctgtt taccttggtg cagaggagca atggggagtg taaagaggca ctagcaaagt 181 ccgagatgaa tgtgaatatg aagtatcagc ttcccaactt caccgcggaa acacccatcc 241 agaatgtcat tctacatgag catcacattt tccttggtgc cactaactac atttatgttt 301 taaatgagga agaccttcag aaggttgctg agtacaagac tgggcctgtg ctggaacacc 361 cagattgttt cccatgtcag gactgcagca gcaaagccaa tttatcagga ggtgtttgga 421 aagataacat caacatggct ctagttgtcg acacctacta tgatgatcaa ctcattagct 481 gtggcagcgt caacagaggg acctgccagc gacatgtctt tccccacaat catactgctg 541 acatacagtc ggaggttcac tgcatattct ccccacagat agaagagccc agccagtgtc 601 ctgactgtgt ggtgagcgcc ctgggagcca aagtcctttc atctgtaaag gaccggttca 661 tcaacttctt tgtaggcaat accataaatt cttcttattt cccagatcat ccattgcatt 721 cgatatcagt gagaaggcta aaggaaacga aagatggttt tatgtttttg acggaccagt 781 cctacattga tgttttacct gagttcagag attcttaccc cattaagtat gtccatgcct 841 ttgaaagcaa caattttatt tacttcttga cggtccaaag ggaaactcta gatgctcaga 901 cttttcacac aagaataatc aggttctgtt ccataaactc tggattgcat tcctacatgg 961 aaatgcctct ggagtgtatt ctcacagaaa agagaaaaaa gagatccaca aagaaggaag 1021 tgtttaatat acttcaggct gcgtatgtca gcaagcctgg ggcccagctt gctagacaaa 1081 taggagccag cctgaatgat gacattcttt tcggggtgtt cgcacaaagc aagccagatt 1141 ctgccgaacc aatggatcga tctgccatgt gtgcattccc tatcaaatat gtcaacgact 1201 tcttcaacaa gatcgtcaac aaaaacaatg tgagatgtct ccagcatttt tacggaccca 1261 atcatgagca ctgctttaat aggacacttc tgagaaattc atcaggctgt gaagcgcgcc 1321 gtgatgaata tcgaacagag tttaccacag ctttgcagcg cgttgactta ttcatgggtc 1381 aattcagcga agtcctctta acatctatat ccaccttcat taaaggagac ctcaccatag 1441 ctaatcttgg gacatcagag ggtcgcttca tgcaggttgt ggtttctcga tcaggaccat 1501 caacccctca tgtgaatttt ctcctggact cccatccagt gtctccagaa gtgattgtgg 1561 agcatacatt aaaccaaaat ggctacacac tggttatcac tgggaagaag atcacgaaga 1621 tcccattgaa tggcttgggc tgcagacatt tccagtcctg cagtcaatgc ctctctgccc 1681 caccctttgt tcagtgtggc tggtgccacg acaaatgtgt gcgatcggag gaatgcctga 1741 gcgggacatg gactcaacag atctgtctgc ctgcaatcta caaggttttc ccaaatagtg 1801 caccccttga aggagggaca aggctgacca tatgtggctg ggactttgga tttcggagga 1861 ataataaatt tgatttaaag aaaactagag ttctccttgg aaatgagagc tgcaccttga 1921 ctttaagtga gagcacgatg aatacattga aatgcacagt tggtcctgcc atgaataagc 1981 atttcaatat gtccataatt atttcaaatg gccacgggac aacacaatac agtacattct 2041 cctatgtgga tcctgtaata acaagtattt cgccgaaata cggtcctatg gctggtggca 2101 ctttacttac tttaactgga aattacctaa acagtgggaa ttctagacac atttcaattg 2161 gtggaaaaac atgtacttta aaaagtgtgt caaacagtat tctcgagtgt tataccccag 2221 cccaaaccat ttcaactgag tttgctgtta aattgaaaat tgacttagcc aaccgagaga 2281 caagcatctt cagttaccgt gaagatccca ttgtctatga aattcatcca accaaatctt 2341 ttattagtgg tgggagcaca ataacaggtg ttgggaaaaa cctgaattca gttagtgtcc 2401 cgagaatggt cataaatgtg catgaagcag gaaggaactt tacagtggca tgtcaacatc 2461 gctctaattc agagataatc tgttgtacca ctccttccct gcaacagctg aatctgcaac 2521 tccccctgaa aaccaaagcc tttttcatgt tagatgggat cctttccaaa tactttgatc 2581 tcatttatgt acataatcct gtgtttaagc cttttgaaaa gccagtgatg atctcaatgg 2641 gcaatgaaaa tgtactggaa attaagggaa atgatattga ccctgaagca gttaaaggtg 2701 aagtgttaaa agttggaaat aagagctgtg agaatataca cttacattct gaagccgttt 2761 tatgcacggt ccccaatgac ctgctgaaat tgaacagcga gctaaatata gagtggaagc 2821 aagcaatttc ttcaaccgtc cttggaaaag taatagttca accagatcag aatttcacag 2881 gattgattgc tggtgttgtc tcaatatcaa cagcactgtt attactactt gggtttttcc 2941 tgtggctgaa aaagagaaag caaattaaag atctgggcag tgaattagtt cgctacgatg 3001 caagagtaca cactcctcat ttggataggc ttgtaagtgc ccgaagtgta agcccaacta 3061 cagaaatggt ttcaaatgaa tctgtagact accgagctac ttttccagaa gatcagtttc 3121 ctaattcatc tcagaacggt tcatgccgac aagtgcagta tcctctgaca gacatgtccc 3181 ccatcctaac tagtggggac tctgatatat ccagtccatt actgcaaaat actgtccaca 3241 ttgacctcag tgctctaaat ccagagctgg tccaggcagt gcagcatgta gtgattgggc 3301 ccagtagcct gattgtgcat ttcaatgaag tcataggaag agggcatttt ggttgtgtat 3361 atcatgggac tttgttggac aatgatggca agaaaattca ctgtgctgtg aaatccttga 3421 acagaatcac tgacatagga gaagtttccc aatttctgac cgagggaatc atcatgaaag 3481 attttagtca tcccaatgtc ctctcgctcc tgggaatctg cctgcgaagt gaagggtctc 3541 cgctggtggt cctaccatac atgaaacatg gagatcttcg aaatttcatt cgaaatgaga 3601 ctcataatcc aactgtaaaa gatcttattg gctttggtct tcaagtagcc aaaggcatga 3661 aatatcttgc aagcaaaaag tttgtccaca gagacttggc tgcaagaaac tgtatgctgg 3721 atgaaaaatt cacagtcaag gttgctgatt ttggtcttgc cagagacatg tatgataaag 3781 aatactatag tgtacacaac aaaacaggtg caaagctgcc agtgaagtgg atggctttGG 3841 AAAGTCTGCA AACTCAAAAG TTTACCACCA AGTCAGATGT GTGGTCCTTT GGCGTGCTCC 3901 TCTGGGAGCT GATGACAAGA GGAGCCCCAC CTTATCCTGA CGTAAACACC TTTGATATAA 3961 CTGTTTACTT GTTGCAAGGG AGAAGACTCC TACAACCCGA ATACTGCCCA GACCCCTTAT 4021 ATGAAGTAAT GCTAAAATGC TGGCACCCTA AAGCCGAAAT GCGCCCATCC TTTTCTGAAC 4081 TGGTGTCCCG GATATCAGCG ATCTTCTCTA CTTTCATTGG GGAGCACTAT GTCCATGTGA 4141 ACGCTACTTA TGTGAACGTA AAATGTGTCG CTCCGTATCC TTCTCTGTTG TCATCAGAAG 4201 ATAACGCTGA TGATGAGGTG GACACACGAC CAGCCTCCTT CTGGGAGACA TCATAGAAGC 4261 TTAAGGGTGG GCGCGCCGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 4321 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 4381 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGCCAAC ATGACGCCTA 4441 CAAGGAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt