Construct: ORF TRCN0000492212

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020424.2_s317c1
DNA Barcode:
TGCCAACATGACGCCTACAAGGAA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
MET (4233)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492212
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4233 MET MET proto-oncogene, recepto... NM_000245.4 99.9% 100% 2121T>C;2124A>G
2 human 4233 MET MET proto-oncogene, recepto... NM_001127500.3 98.6% 98.7% 2121T>C;2124A>G;2263_2316del
3 human 4233 MET MET proto-oncogene, recepto... XM_011516223.1 98.6% 98.6% 1_57del;2178T>C;2181A>G
4 human 4233 MET MET proto-oncogene, recepto... NM_001324402.2 69% 69% 0_1ins1290;831T>C;834A>G
5 human 4233 MET MET proto-oncogene, recepto... XM_006715990.2 69% 69% 0_1ins1290;831T>C;834A>G
6 human 4233 MET MET proto-oncogene, recepto... NM_001324401.2 66.6% 65.9% (many diffs)
7 human 4233 MET MET proto-oncogene, recepto... XR_001744772.1 61% (many diffs)
8 mouse 17295 Met met proto-oncogene NM_008591.2 85.7% 88.9% (many diffs)
9 mouse 17295 Met met proto-oncogene XM_011241042.2 85.7% 88.9% (many diffs)
10 mouse 17295 Met met proto-oncogene XM_011241043.2 85.7% 88.9% (many diffs)
11 mouse 17295 Met met proto-oncogene XM_006505002.3 82.4% 85.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
84
ORF end:
4254
ORF length:
4170
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggctccgc ggcccccttc accatgaagg cccccgctgt gcttgcacct ggcatcctcg
121tgctcctgtt taccttggtg cagaggagca atggggagtg taaagaggca ctagcaaagt
181ccgagatgaa tgtgaatatg aagtatcagc ttcccaactt caccgcggaa acacccatcc
241agaatgtcat tctacatgag catcacattt tccttggtgc cactaactac atttatgttt
301taaatgagga agaccttcag aaggttgctg agtacaagac tgggcctgtg ctggaacacc
361cagattgttt cccatgtcag gactgcagca gcaaagccaa tttatcagga ggtgtttgga
421aagataacat caacatggct ctagttgtcg acacctacta tgatgatcaa ctcattagct
481gtggcagcgt caacagaggg acctgccagc gacatgtctt tccccacaat catactgctg
541acatacagtc ggaggttcac tgcatattct ccccacagat agaagagccc agccagtgtc
601ctgactgtgt ggtgagcgcc ctgggagcca aagtcctttc atctgtaaag gaccggttca
661tcaacttctt tgtaggcaat accataaatt cttcttattt cccagatcat ccattgcatt
721cgatatcagt gagaaggcta aaggaaacga aagatggttt tatgtttttg acggaccagt
781cctacattga tgttttacct gagttcagag attcttaccc cattaagtat gtccatgcct
841ttgaaagcaa caattttatt tacttcttga cggtccaaag ggaaactcta gatgctcaga
901cttttcacac aagaataatc aggttctgtt ccataaactc tggattgcat tcctacatgg
961aaatgcctct ggagtgtatt ctcacagaaa agagaaaaaa gagatccaca aagaaggaag
1021tgtttaatat acttcaggct gcgtatgtca gcaagcctgg ggcccagctt gctagacaaa
1081taggagccag cctgaatgat gacattcttt tcggggtgtt cgcacaaagc aagccagatt
1141ctgccgaacc aatggatcga tctgccatgt gtgcattccc tatcaaatat gtcaacgact
1201tcttcaacaa gatcgtcaac aaaaacaatg tgagatgtct ccagcatttt tacggaccca
1261atcatgagca ctgctttaat aggacacttc tgagaaattc atcaggctgt gaagcgcgcc
1321gtgatgaata tcgaacagag tttaccacag ctttgcagcg cgttgactta ttcatgggtc
1381aattcagcga agtcctctta acatctatat ccaccttcat taaaggagac ctcaccatag
1441ctaatcttgg gacatcagag ggtcgcttca tgcaggttgt ggtttctcga tcaggaccat
1501caacccctca tgtgaatttt ctcctggact cccatccagt gtctccagaa gtgattgtgg
1561agcatacatt aaaccaaaat ggctacacac tggttatcac tgggaagaag atcacgaaga
1621tcccattgaa tggcttgggc tgcagacatt tccagtcctg cagtcaatgc ctctctgccc
1681caccctttgt tcagtgtggc tggtgccacg acaaatgtgt gcgatcggag gaatgcctga
1741gcgggacatg gactcaacag atctgtctgc ctgcaatcta caaggttttc ccaaatagtg
1801caccccttga aggagggaca aggctgacca tatgtggctg ggactttgga tttcggagga
1861ataataaatt tgatttaaag aaaactagag ttctccttgg aaatgagagc tgcaccttga
1921ctttaagtga gagcacgatg aatacattga aatgcacagt tggtcctgcc atgaataagc
1981atttcaatat gtccataatt atttcaaatg gccacgggac aacacaatac agtacattct
2041cctatgtgga tcctgtaata acaagtattt cgccgaaata cggtcctatg gctggtggca
2101ctttacttac tttaactgga aattacctaa acagtgggaa ttctagacac atttcaattg
2161gtggaaaaac atgtacttta aaaagtgtgt caaacagtat tctcgagtgt tataccccag
2221cccaaaccat ttcaactgag tttgctgtta aattgaaaat tgacttagcc aaccgagaga
2281caagcatctt cagttaccgt gaagatccca ttgtctatga aattcatcca accaaatctt
2341ttattagtgg tgggagcaca ataacaggtg ttgggaaaaa cctgaattca gttagtgtcc
2401cgagaatggt cataaatgtg catgaagcag gaaggaactt tacagtggca tgtcaacatc
2461gctctaattc agagataatc tgttgtacca ctccttccct gcaacagctg aatctgcaac
2521tccccctgaa aaccaaagcc tttttcatgt tagatgggat cctttccaaa tactttgatc
2581tcatttatgt acataatcct gtgtttaagc cttttgaaaa gccagtgatg atctcaatgg
2641gcaatgaaaa tgtactggaa attaagggaa atgatattga ccctgaagca gttaaaggtg
2701aagtgttaaa agttggaaat aagagctgtg agaatataca cttacattct gaagccgttt
2761tatgcacggt ccccaatgac ctgctgaaat tgaacagcga gctaaatata gagtggaagc
2821aagcaatttc ttcaaccgtc cttggaaaag taatagttca accagatcag aatttcacag
2881gattgattgc tggtgttgtc tcaatatcaa cagcactgtt attactactt gggtttttcc
2941tgtggctgaa aaagagaaag caaattaaag atctgggcag tgaattagtt cgctacgatg
3001caagagtaca cactcctcat ttggataggc ttgtaagtgc ccgaagtgta agcccaacta
3061cagaaatggt ttcaaatgaa tctgtagact accgagctac ttttccagaa gatcagtttc
3121ctaattcatc tcagaacggt tcatgccgac aagtgcagta tcctctgaca gacatgtccc
3181ccatcctaac tagtggggac tctgatatat ccagtccatt actgcaaaat actgtccaca
3241ttgacctcag tgctctaaat ccagagctgg tccaggcagt gcagcatgta gtgattgggc
3301ccagtagcct gattgtgcat ttcaatgaag tcataggaag agggcatttt ggttgtgtat
3361atcatgggac tttgttggac aatgatggca agaaaattca ctgtgctgtg aaatccttga
3421acagaatcac tgacatagga gaagtttccc aatttctgac cgagggaatc atcatgaaag
3481attttagtca tcccaatgtc ctctcgctcc tgggaatctg cctgcgaagt gaagggtctc
3541cgctggtggt cctaccatac atgaaacatg gagatcttcg aaatttcatt cgaaatgaga
3601ctcataatcc aactgtaaaa gatcttattg gctttggtct tcaagtagcc aaaggcatga
3661aatatcttgc aagcaaaaag tttgtccaca gagacttggc tgcaagaaac tgtatgctgg
3721atgaaaaatt cacagtcaag gttgctgatt ttggtcttgc cagagacatg tatgataaag
3781aatactatag tgtacacaac aaaacaggtg caaagctgcc agtgaagtgg atggctttGG
3841AAAGTCTGCA AACTCAAAAG TTTACCACCA AGTCAGATGT GTGGTCCTTT GGCGTGCTCC
3901TCTGGGAGCT GATGACAAGA GGAGCCCCAC CTTATCCTGA CGTAAACACC TTTGATATAA
3961CTGTTTACTT GTTGCAAGGG AGAAGACTCC TACAACCCGA ATACTGCCCA GACCCCTTAT
4021ATGAAGTAAT GCTAAAATGC TGGCACCCTA AAGCCGAAAT GCGCCCATCC TTTTCTGAAC
4081TGGTGTCCCG GATATCAGCG ATCTTCTCTA CTTTCATTGG GGAGCACTAT GTCCATGTGA
4141ACGCTACTTA TGTGAACGTA AAATGTGTCG CTCCGTATCC TTCTCTGTTG TCATCAGAAG
4201ATAACGCTGA TGATGAGGTG GACACACGAC CAGCCTCCTT CTGGGAGACA TCATAGAAGC
4261TTAAGGGTGG GCGCGCCGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
4321TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
4381GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGCCAAC ATGACGCCTA
4441CAAGGAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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